NIPPON SUISAN GAKKAISHI
Online ISSN : 1349-998X
Print ISSN : 0021-5392
ISSN-L : 0021-5392
Volume 82, Issue 2
Displaying 1-50 of 61 articles from this issue
Obituary
Foreword
Originals
  • TARO TEGA, KENTARO NIWA, SEINEN CHOW
    2016 Volume 82 Issue 2 Pages 102-111
    Published: 2016
    Released on J-STAGE: April 04, 2016
    Advance online publication: February 18, 2016
    JOURNAL FREE ACCESS
     Peptide nucleic acid (PNA)-directed PCR clamping was used to efficiently detect prey animals in the predators' stomach. Two oligo PNAs (19 and 18 mer) having sequences specific to the 16S rDNAs of olive flounder Paralichthys olivaceus and bigfin reef squid Sepioteuthis lessoniana were designed. PCR amplification of the 16S rDNAs of these species was successfully inhibited in the presence of these PNAs. This method was applied to DNAs extracted from the stomach contents of olive flounder and bigfin reef squid juveniles caught in Tsuruga Bay, Japan. Goby DNAs were detected in nine of 11 flounder juveniles examined, and DNAs of the Japanese anchovy Engraulis japonicus and the Japanese silver-biddy Gerres oyena were detected in each of the remaining founder juveniles. Anchovy DNA was also detected in two of nine squid juveniles, while fish species could not be identified for short sequences (118 bp) obtained from the other squid juveniles.
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  • HIROKO TAKAOKA, SYUHEI ODORIBA, KIYOMI MURAKUMO, RYO NOZU, MASARU NAKA ...
    2016 Volume 82 Issue 2 Pages 112-118
    Published: 2016
    Released on J-STAGE: April 04, 2016
    Advance online publication: February 25, 2016
    JOURNAL FREE ACCESS
     In order to restore the stock of Lavender jobfish Pristipomoides sieboldii, we investigated differences in ovarian histology and serum sex steroid hormone (testosterone and 17-beta estradiol) levels between the time of collection and after 24 hours of refrigeration. Additionally, the maturation process of eggs and differences in ovary location-dependent egg development were examined. In mid-May 2012, 12 females were caught by fishing at Shirama-zone near Ie Island, Okinawa. Gonads and blood were collected immediately after fishing and again after 24 hours of refrigeration. Histological observation of the ovaries revealed that there was no difference in location-dependent egg development, and that oogenic germ cells from oogonia to mature oocytes were localized in the ovary simultaneously. Additionally, we showed that ovarian tissue did not exhibit histological differences, whereas sex steroid hormone levels differed between the time of collection and after 24 hours of refrigeration.
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  • TAKASHI IWASAKI, KENTARO IDE, JUN SATO, KATSUYUKI HAMASAKI
    2016 Volume 82 Issue 2 Pages 119-127
    Published: 2016
    Released on J-STAGE: April 04, 2016
    Advance online publication: February 26, 2016
    JOURNAL FREE ACCESS
     To know the optimal feeding schedule for culturing larvae of longtooth grouper Epinephelus bruneus and sevenband grouper E. septemfasciatus during seed production, we examined the food selectivity for rotifers Brachionus plicatilis species complex and Artemia nauplii by larvae reared in a hatchery. The larvae of both species with ≥8 mm standard length showed a preference for Artemia nauplii and the mouth width of larvae with minimum body sizes fed with Artemia nauplii was nearly equal to the body width of Artemia nauplii. Therefore, it was suggested that the mouth width of larvae of both species is the restricting factor for preying on Artemia nauplii. This finding will help improve the feeding schedule of Artemia nauplii during the seed production process of these commercially important grouper species.
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Short Papers
Symposia
JSFS Executive board special symposium in 2015
Recent situation of the Tohoku regional Ocean —Research activities and contribution to reconstruction ...
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II.
Development of new technologies for innovation in fisheries related to the reconstruction and renovation efforts following the Great East Japan ...
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II.
III.
Mini-Symposium
Ensuring the safety of pufferfish consumption — A review of the taxonomy and toxicity of pufferfish from Japanese coastal areas
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