RADIOISOTOPES

Use of Resin-strip for the Determination of Unsaturated lron-binding Capacity

Hiroshi SAITO

1970Volume 19Issue 4 Pages 179-183
Published: April 15, 1970
Released on J-STAGE: September 07, 2010

DOIhttps://doi.org/10.3769/radioisotopes.19.4_179

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The resin-strip made of Amberlite IRA 400 was used for the elimination of iron ion added to serum to determine unsaturated iron-binding capacity (UIBC) . More than 95% of iron ion in the serum was eliminated within one hour with two resin-strips of 0.4 gram wet weight. No significant effect of heparin, iron ion concentration, and temperature to UIBC values was observed. UIBC values obtained by the resin-strip were in good agreement with those by magnesium carbonate powder.
The resin-strip is picked out before counting sample and the procedure is largely simplified; centrifugation, pipetting for supernatant counting, and addition of buffer solution are not needed.

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Removal of Mercury Bound to Hemoglobin by Chelating Agents Containing Sulfhydryl Group

Yukio SUGIURA, Yasushi HOJO, Hisashi TANAKA

1970Volume 19Issue 4 Pages 184-189
Published: April 15, 1970
Released on J-STAGE: September 07, 2010

DOIhttps://doi.org/10.3769/radioisotopes.19.4_184

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Removal of mercury, bound to sulfhydryl groups of hemoglobin, by various Chelating agents containing sulfhydryl group, was investigated by the use of equilibrium dialysis. Chelating agents employed were cysteine, penicillamine, glutathione, mercaptoacetic acid and their related compounds. Both inorganic and organic mercury compounds were most effectively removed at physiological pH. As to ethylmercuric chloride, linear correlation was found between the percentage of removal and the dissociation constants of sulf hydryl group of the chelating agents. The effect of the sulfur-oxygen coordinating chelating agent was about 20% greater than that of the sulfur-nitrogen coordinating chelating agent. On the other hand, the removal of mercuric chloride from hemoglobin was independent of the acid dissociation constants of the chelating agents. The effect of the isosteric substitution of the coordinating atom was seen by the fact that the ability of the removal of mercury decreases in the order selenocysteamine > cysteamine >> ethanolamine for ethylmercuric chloride, and cysteamine > selenocysteamine >> ethanolamine for mercuric chloride. A ternary complex, hemoglobin-mercury-cysteine complex, which is considered to be an important intermediate in the removal of mercury was found by the gel-filtration technique.

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A Tumor-specific Localizing Agent for Radioisotope lmage (III)

—The Synthesis of 5-lodocytosine-¹³¹I and lts Distribution in Tumor Bearing Mice—

Tatsuo MAEDA, Isao WATANABE, Itsuma KAMOI, Masaharu KOJIMA, Keiko KUDO ...

1970Volume 19Issue 4 Pages 190-193
Published: April 15, 1970
Released on J-STAGE: September 07, 2010

DOIhttps://doi.org/10.3769/radioisotopes.19.4_190

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5-iodocytosine-¹³¹I was synthesized by the exchange reaction of 5-IC with Na¹³¹I. Specific activity of synthesized 5-¹³¹IC was 450 μCi/mg (0. 34 mCi/ml) . The autoradiolysis was 7% in 30 days.
Studies of distribution of 5-¹³¹IC in mice and scintiphotography indicated that the intraperitoneally injected 5-131IC was incorporated into the stomach, lung and tumor, and excreted to the kidney. The whole body macroautoradiography showed the incorporation of 5-¹³¹IC into the implanted tumor in mice (6 hr after intraperitoneal injection) .

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The Determination of Uranium Content of Standard Rocks by Fission-Track Registration in Muscovite

Susumu NISHIMURA

1970Volume 19Issue 4 Pages 194-196
Published: April 15, 1970
Released on J-STAGE: July 21, 2010

DOIhttps://doi.org/10.3769/radioisotopes.19.4_194

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Effects of γ-ray Irradiation on Treeing in Organic Insulating Materials

Shigeo KOBAYASHI, Yoshiteru SHIMIZU

1970Volume 19Issue 4 Pages 197-200
Published: April 15, 1970
Released on J-STAGE: July 21, 2010

DOIhttps://doi.org/10.3769/radioisotopes.19.4_197

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[title in Japanese]

[in Japanese]

1970Volume 19Issue 4 Pages 201-209
Published: April 15, 1970
Released on J-STAGE: July 21, 2010

DOIhttps://doi.org/10.3769/radioisotopes.19.4_201

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[title in Japanese]

[in Japanese], [in Japanese]

1970Volume 19Issue 4 Pages 210-211
Published: April 15, 1970
Released on J-STAGE: July 21, 2010

DOIhttps://doi.org/10.3769/radioisotopes.19.4_210

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[title in Japanese]

[in Japanese]

1970Volume 19Issue 4 Pages 212-218
Published: April 15, 1970
Released on J-STAGE: July 21, 2010

DOIhttps://doi.org/10.3769/radioisotopes.19.4_212

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[title in Japanese]

[in Japanese]

1970Volume 19Issue 4 Pages 218-221
Published: April 15, 1970
Released on J-STAGE: July 21, 2010

DOIhttps://doi.org/10.3769/radioisotopes.19.4_218

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[title in Japanese]

1970Volume 19Issue 4 Pages A1043-A608
Published: April 15, 1970
Released on J-STAGE: July 21, 2010

DOIhttps://doi.org/10.3769/radioisotopes.19.4_A1043

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