RADIOISOTOPES

Whole Body Autoradiographic Studies on the Distribution of Radioisotopes (XXI)

Fate of ¹⁴C-Arginine in Mice

Tadao TAKAHASHI, Tetsuo KIMURA, Yoshishige SATO

1970Volume 19Issue 8 Pages 353-359
Published: August 15, 1970
Released on J-STAGE: September 07, 2010

DOIhttps://doi.org/10.3769/radioisotopes.19.8_353

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The metabolic fate of ¹⁴C-arginine was studied in mice. The highest concentration of radioactivity 60 minutes after intravenous injection of ¹⁴C-arginine was found in the pancreas, gastrointestinal wall, kidney and spleen. Moderate levels of radioactivity were observed in the adrenal, skin, hypophysis, thymus and testis, while radioactivity in the liver, lung, brain, blood and brown fat was low. Twenty-four hours later, the levels of radioactivity in the organs were markedly low.
The concentration of radioactivity in the trichloroacetic acid (TCA) -soluble fraction so minutes after injection was found to be highest in the kidney, moderately high in the muscle, pancreas and salivary glands, and low in the brain. In the TCA-insoluble protein fraction the highest concentration of radioactivity was found in the pancreas and intestinal wall 60 minutes after injection. The levels of radioactivity of the TCA soluble fraction in various tissues after 24 hours were markedly low but the incorporation of radioactive substances into proteins of the testis, muscle and brain were great.
Seventy-four per cent of the radioactivity administered was excreted in the 24-hour urine of mice. Approximately 75% of the urinary radioactivity was present in the form of ¹⁴C-urea and about s % was unchanged ¹⁴C-arginine. The cumulative ¹⁴CO² exhaled in 24 hours was 8.3% of the administered radioactivity.

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Autoradiographic Studies of Epidermal Amino Acid Metabolism

II. In the Human Epidermis of Skin Diseases

Yoshio OHSHIMA

1970Volume 19Issue 8 Pages 360-366
Published: August 15, 1970
Released on J-STAGE: September 07, 2010

DOIhttps://doi.org/10.3769/radioisotopes.19.8_360

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The incorporation of tritiated amino acids in the human epidermis of some dermatoses was investigated in vitro and in vivo by means of autoradiography.
In the parakeratotic epidermis associated with hyperkeratosis and acanthosis, i.e, psoriasis, chronic dermatitis, verruca vulgaris, etc., the radioactive particles of tritiated tyrosine, phenylalanine, valine and leucine are found in both the upper and lower spinous cell layers after one and two hour incubation, while they are observed only in the lower spinous cell layer in the normal epidermis. The similar tendency is found in the epidermis with hyperand parakeratotis induced by radiation of ultraviolet rays or stripping of the horny layer. The more parakeratotic changes, the more is the tendency. It is suggested therefore that the protein synthesis or metabolism occurring in each level of the epidermis is accelerated by shortened life-span in the parakeratotic epidermis.
In ichthyosis vulgaris, the so-called keratogenous zone is distinctly demonstrated in narrow width immediately beneath the horny layer, showing the fairly intensive labeling from tritiated glycine.
In the dyskeratotic cells of skin cancer and molluscum contagiosum, few silver grains are distributed with tritiated methionine, histidine, phenylalanine, valine and leucine.

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Extraction of ³H-labeled Aniline from ³H-labeled Aniline Substituted Poly (Vinylidene Chloride-Vinyl Chloride)

Sumio YAMADA, Tsutomu NAKAGAWA

1970Volume 19Issue 8 Pages 367-370
Published: August 15, 1970
Released on J-STAGE: July 21, 2010

DOIhttps://doi.org/10.3769/radioisotopes.19.8_367

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Insulin Radioimmunoassay Using “INSULIN KIT”

Ryohei KATAOKA, Masaaki KASHIMA, Motoko MIKAWA

1970Volume 19Issue 8 Pages 371-373
Published: August 15, 1970
Released on J-STAGE: July 21, 2010

DOIhttps://doi.org/10.3769/radioisotopes.19.8_371

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Radioimmunoassay of Serum Insulin by “INSULIN KIT”

Takao WAKABAYASHI

1970Volume 19Issue 8 Pages 374-376
Published: August 15, 1970
Released on J-STAGE: July 21, 2010

DOIhttps://doi.org/10.3769/radioisotopes.19.8_374

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Comparison of Insulin Radioimmunoassay by “INSULIN KIT” and Morgan-Lazarow's Method

Takeshi KUZUYA

1970Volume 19Issue 8 Pages 377
Published: August 15, 1970
Released on J-STAGE: July 21, 2010

DOIhttps://doi.org/10.3769/radioisotopes.19.8_377

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Nucleonic Application in Space and Ocean

Masao KATO

1970Volume 19Issue 8 Pages 378-387
Published: August 15, 1970
Released on J-STAGE: July 21, 2010

DOIhttps://doi.org/10.3769/radioisotopes.19.8_378

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Radiation Safety Technique (VIII), Radiochemical Techniques for Radioisotope Users

Noboru YAMAGATA

1970Volume 19Issue 8 Pages 388-397
Published: August 15, 1970
Released on J-STAGE: July 21, 2010

DOIhttps://doi.org/10.3769/radioisotopes.19.8_388

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[title in Japanese]

1970Volume 19Issue 8 Pages A1075-A972
Published: August 15, 1970
Released on J-STAGE: July 21, 2010

DOIhttps://doi.org/10.3769/radioisotopes.19.8_A1075

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