13C-glucose is consisted of carbon 13, stable isotope. Six carbons in glucose were converted to
13C.
13C-glucose breath test (
13C-GBT) is useful for evaluating the cell viability and human metabolic function. It is assessed by
13CO
2, metabolite of
13C-glucose, in exhausted gas samples. It is quickly measured by using an isotope ratio mass spectrometer (POC one, Otsuka Pharmaceutical Co. Ltd, Tokyo).
In the study we made, we have assessed glucose metabolism in the reconstructed liver organoid using by
13C-GBT. We made a 3-dimentional reconstructed organoid (mini artificial liver model) using a radial-flow bioreactor(RFB), and evaluated viability of the organoid by
13C-GBT. The liver organoid was constructed by co-culture with immortalized mouse hepatocytes, sinusoidal endothelial cells and hepatic stellate cells. Exhaust gas was sampled in gas bags from the closed circuit of RFB system.
Firstly we observed transition of
13CO
2 in exhaust gas along culture time. Glucose metabolism was enhanced by not only hepatocyte growth, but co-culture of non-parenchymal cells. We also examined ischemia/reperfusion injury model in the reconstructed liver organoid.
13C-GBT clearly showed depressed metabolism by ischemic effect to the liver organoid.
In conclusion,
13C-GBT is extremely useful for assessment of viability and glucose metabolism in real-time, easily and high sensitivity not only in vivo but in vitro.
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