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Ikuo Hirono
2000 Volume 66 Issue 4 Pages
603-606
Published: July 15, 2000
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Yasuzumi Fujimori
2000 Volume 66 Issue 4 Pages
607-610
Published: July 15, 2000
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Kiyoshi Kikuchi
2000 Volume 66 Issue 4 Pages
611-614
Published: July 15, 2000
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Tomoo Sawabe
2000 Volume 66 Issue 4 Pages
615-618
Published: July 15, 2000
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Kohji Iida
2000 Volume 66 Issue 4 Pages
619-622
Published: July 15, 2000
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Hideki Tanaka, Hiromi Ohta, Hirohiko Kagawa
2000 Volume 66 Issue 4 Pages
623-626
Published: July 15, 2000
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Akira Nomura
2000 Volume 66 Issue 4 Pages
627-630
Published: July 15, 2000
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Kenichi Watanabe, Takahiro Okazaki
2000 Volume 66 Issue 4 Pages
631-638
Published: July 15, 2000
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The maturation and spawning season of threeline grunt Parapristipoma trilineatum were sampled using 3, 296 specimens collected around Mugi-oshima Island of Tokushima Prefecture, Japan. Gonadal maturation was observed to occur from early May in females, and the middle of April in males. The spawning season was estimated to occur from late May to early July in females, from late May to early June in two-year old males and from late April to the middle of June in males over two years old. The maturity and spawning season in this sea area is early in comparison with that in other areas of the past report. The main factor leading to the rapid development is considered to be the recent high water temperature in this sea area. Respectively, 0% and 100% of females are mature at one and three years old; 76.7% of females, histologically, and 58.3% in the development stage of the ovary are mature at two years old. Most males mature at two years old.
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Kenichi T. Suzuki, Takanori Kobayashi, Takashi Matsuishi, Kenichi Numa ...
2000 Volume 66 Issue 4 Pages
639-646
Published: July 15, 2000
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Mitochondrial DNA (mtDNA) of masu salmon among eight populations from six rivers around Hokkaido island were examined to clarify their genetic features and population structure. Restriction fragment length polymorphism (RFLP) analysis using 10 restriction endonucleases (Acc I, Ava I, BamH I, Bgl I, Bgl II, EcoR I, Hinc II, Hind III, Pst I, Xba I) revealed considerable variation. We found 20 haplotypes in 180 specimens. Restriction patterns were polymorphic for 7 enzymes examined. Nucleotide divergence among haplotypes ranged from 0.14 to 2.41%. Nucleotide divergence among populations ranged from 0.49 to 0.70%. Dendrogram constructed by UPGMA among 20 haplotypes showed no clustering groups of localities. Nucleotide diversity and nucleon diversity of each locality ranged from 0.0043 to 0.0072, and from 0.7617 to 0.9304, respectively. These results showed masu salmon populations in Hokkaido have a high mtDNA variability. It is important that high genetic variability be highly regarded in stock enhancement of masu salmon.
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Tsuneo Isoo, Tatsuhito Takahashi, Mitsumasa Okada
2000 Volume 66 Issue 4 Pages
647-650
Published: July 15, 2000
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The average number and length of Sargassum plants that have settled on the three types of substrata, carbonated steel-making slag block, granite stone, and cement concrete block, deployed from April 1998 to January 1999 at Koune, the Seto Inland Sea of Japan, were compared. Results showed that maximum length of Sargassum plants that have settled on the three types of substrata did not vary significantly, so the strength of adhesion of the plants was the same for all substratum types. On the other hand, the average number of plants that have settled on the test pieces of carbonated steel-making slag block was higher than that on the cement concrete block although not significantly different from that on the granite stone. This suggests that carbonated steel-making slag blocks have good potential as seaweed bed substrata.
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Shigeru Kawamata
2000 Volume 66 Issue 4 Pages
651-657
Published: July 15, 2000
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Transplant experiment was conducted to investigate the developmental tolerance to wave-induced dislodgement for Laminaria japonica in response to water movement. L. japonica plants cultured on long-line rope in a sheltered site were moved to a moderately exposed site monthly from February to May. Attachment strength for plants from the sheltered site increased linearly with the plant weight but was much lower than that for transplants with the same plant weight. The ratio of attachment strength to plant weight in late June showed a linear increase with the days of cultivation at the transplant site. Comparison of the frequency distribution of the root-mean-square water velocity at the transplant site with that at the sheltered site suggested that there might exist the velocity threshold of developmental tolerance to dislodgement, probably from 15 to 20cm/s in rms value.
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Kenichiro Sato, Natsuko Kajiwara, Shinya Hashimoto, Hideaki Kidokoro, ...
2000 Volume 66 Issue 4 Pages
658-665
Published: July 15, 2000
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The world-wide distribution of squid has led researchers to propose that these animals be used for biomonitoring of persistent organic chemicals in the open ocean. However, their utility as a bioindicator species has not been examined in detail. The present study examined the accumulative characteristics of or ganochlorine compounds (OCs) in the Japanese common squid Todarodes pacificus. Sampling was done in the Japan Sea and East China Sea, with a focus on whether contaminant levels in squid reflected those in surface waters at particular sampling sites. Accumulation of OCs was primarily in the liver, and concentrations did not vary with stage of maturation of the animals. This suggests that an apparent equilibrium of OCs between liver and seawater may occur in a relatively short time at each sampling area. Moreover, we found that it may be possible to estimate the concentrations of OCs in seawater from levels of α-HCH and PCB congener (Cl
5-Cl
9) in squid liver, because concentrations of these compounds in squid liver displayed a significant linear correlation with concentrations in seawater of 1-2 later years at almost the same area. The present study indicates that the Squid Watch program using T. pacificus can be a useful biomonitoring method to estimate OCs levels in surface seawater of the open ocean.
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Keizo Nagasaki, Mineo Yamaguchi, Ichiro Imai
2000 Volume 66 Issue 4 Pages
666-673
Published: July 15, 2000
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Algicidal activity of a bacterium strain Cytophaga sp. AA8-2 against the harmful red tide causing alga Heterocapsa circularisquama was investigated. Physiological conditions of host cells, incubation temperature and existence of ambient organic substrates or co-existing bacteria affected the lethal effect of Cytophaga sp. AA8-2. Bacterial lysis of H. circularisquama was caused more rapidly at higher incubation temperature (20-30°C). Growth of 6 among 7 H. circularisquama strains tested was inhibited by Cytophaga sp. AA8-2, the levels of which were varied. A part of H. circularisquama cells in a culture formed temporary cysts to survive the bacterial attack. The envelope of the temporary cyst of H. circularisquama was composed of a markedly thicker layered structure (209±72nm) than that of the vegetative cell (40±15nm).
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Hongwei Ye, Sadami Yada, Hu Chen
2000 Volume 66 Issue 4 Pages
674-681
Published: July 15, 2000
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The cutting edge angle of a knife is an important factor, affecting the cutting performance. Therefore, it is necessary to measure the cutting edge angle of a fresh fish knife comparing to cutting performance. This paper described a new measuring system of cutting edge angle using a laser light, and the measuring precision. The results are summarized as follows. 1. The cutting edge angle of seven single-edge knives of three kinds, was not uniform, which might be the result of handwork. 2. The influence of laser light beam diffusion on measuring precision is very small. The measurement error was about 0.1°. 3. It is possible to use the method for sampling inspection in the production process, and it makes a major contribution to the uniformity of the products.
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Hisayuki Mikami, Tohru Mukai, Kohji Iida
2000 Volume 66 Issue 4 Pages
682-689
Published: July 15, 2000
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In recent years, acoustic technology has been extensively used for estimating krill abundance. This acoustic assessment is required for precise estimate of target strength (TS) of a krill. However, prediction of the target strength from the theoretical scattering models is generally dependent on the swimming angle, the density and sound speed contrasts between krill and seawater. In these parameters, the density contrasts and sound speed contrasts are known to change during the year. In this study, the seasonal variations of the specific density and sound speed contrasts of Euphausia pacifica are presented. The specific densities of E. pacifica were measured in a series of saline or glycerol solutions of different densities. The measurements of the sound speed were performed using T-shaped velocimeter with two transducers mounted at the ends of the horizontal tube. The specific densities of E. pacifica changed about 1% with season. The sound speed contrasts also varied about 3% with season. These seasonal changes yielded difference about 5dB in target strength of E. pacifica calculated from a straight cylinder model.
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Kenichi Watanabe, Tatuya Hosho, Kouzi Saiura, Takahiro Okazaki, Yoshih ...
2000 Volume 66 Issue 4 Pages
690-696
Published: July 15, 2000
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Fisheries management of Parapristipoma trilineatum around Mugi-oshima Island on the Pacific coast of Tokushima Prefecture was examined based on spawning per recruit analysis. %SPR of the present stock is 18.6%. The minimum size limit of the present fisheries regulation is 20cm FL, and fish under the minimum size must be released. The ideal status (%SPR=30 or 35%) can be attained by reducing the fishing coefficient on two year old fish by 54.4% or 73.0%, respectively, and prohibiting fishing on fish younger than one year old. The target %SPR can be gained by raising the minimum size from 20cm to 23cm or 24cm.
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Gwang Sic Park, Toshio Takeuchi, Tadahisa Seikai, Tomoyoshi Yoshinaga
2000 Volume 66 Issue 4 Pages
697-704
Published: July 15, 2000
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This experiment was conducted to investigate the effects of residual salts (mainly NaCl) and free amino acids in mysid Archaeomysis vulgaris meal on the growth of juvenile Japanese flounder Paralichthys olivaceus. Five experimental diets were designed. Diet 3 contained fish meal as sole protein source. Fish meal was replaced by de-salted mysid meal at the level of 37.5% and 77.5% in diets 1 and 2 respectively. Diet 4 contained 82.0% mysid meal and diet 5 was a commercial diet. Fish with mean body weight 0.16g (experiment I) and 1.35g (experiment II) were fed on the above diets for 14 days, respectively. Both size of juvenile flounder (0.16 and 1.35g) fed the 77.5% de-salted mysid meal diet showed significantly better (p<0.05) growth than that fed the 82.0% mysid meal diet. These results clearly indicated that the excess amount of salts in the diet reduced the growth of juvenile flounder. On the other hand, the best growth was shown in the fish fed the 37.5% de-salted mysid meal diet containing 81.4mg/g free amino acids (FAA) in total nitrogen compounds. The FAA analyses of fish total nitrogen compounds showed a similar profile to 37.5% de-salted mysid meal diet. These results suggested that growth of Japanese flounder juvenile was found to be affected by dietary FAA levels in de-salted mysid meal.
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Hirotaka Otobe, Yasuaki Takagi, Ayako Anbo
2000 Volume 66 Issue 4 Pages
705-712
Published: July 15, 2000
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The relationship between daily salmon catch and sea and weather conditions in Otsuchi Bay located at the center of the Sanriku Coast was analyzed in 1996 and 1997. The daily salmon catch reached a peak when the sea water temperature reached 13°C level in both years. In 80% of cases, the salmon catch at the day after a rainfall (>5mm) or strong westerly wind (>10m/s) rose to over 1.5 times that of the previous day. The largest salmon catch of each year was observed to be on the day after the largest precipitation or the longest strong westerly wind. These data suggest that the homing behavior of chum salmon was affected significantly by sea water temperature, rainfall and westerly wind on the Sanriku Coast.
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Takayuki Kusakabe, Masaki Nakajima, Masaki Sano, Kazuo Watanabe
2000 Volume 66 Issue 4 Pages
713-718
Published: July 15, 2000
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The vertical distribution and feeding of Japanese sand lance Ammodytes personatus larvae were examined based on the data obtained by 5 times of MTD depth-discrete net tows in Osaka Bay during daytime in January 1991, January and February 1992, and January 1993. Vertical distributions of light intensity, temperature, salinity, and copepods abundance were also measured. Sand lance larvae were abundant in 1-10m depth (especially at 5m depth), while the vertical profiles of temperature and salinity were not stratified. The value of fullness of gut of larvae (F. G.) declined with increasing depth, whereas the vertical distribution of copepods was not consistent with F. G. The data from laboratory experiments demonstrated that light intensity lower than (10)
2lx is insufficient for feeding of sand lance larvae (14 days after hatching, mean T. L.=6.8mm) on rotifers. In the field, light intensity lower than (10)
2lx occurred at 15-20m depth. These facts suggest that the distribution depth of sand lance larvae during daytime is strongly related to light intensity necessary for feeding.
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Yasuyuki Tsukamasa, Yasuyoshi Miyake, Masashi Ando, Yasuo Makinodan
2000 Volume 66 Issue 4 Pages
719-725
Published: July 15, 2000
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Carp meat has weak gel-forming and slow setting properties. To clarify the factors of such properties seems to be necessary to utilize fish meat like carp for making kamaboko. Meat sols were prepared in various combination with CaCl
2, (transglutaminase (TGase) activator), EGTA, (TGase and metallo-endopeptidase inhibitor), and E-64, (cystein-endopeptidase inhibitor). Sols were incubated at 30, 40 and 50°C (first step), then heated at 85°C for 0.5h (second step). First step sols at 30°C did not set, however, breaking strength of second step gels were improved with CaCl
2. At 40°C, CaCl
2+E-64 improved breaking strength of gels remarkably and EGTA+E-64 did so slightly. At 50°C, EGTA, CaCl
2+E-64 and EGTA+E-64 improved breaking strength of gels greatly. E-64 inhibited degradation of myosin heavy chain (HC) at 40 and 50°C, and EGTA did partially at 50°C. Endogenous TGase catalyzed HC polymerization even at 50°C in the presence of E-64. Optimum temperature of TGase was around 40°C, however, high activity remained even at 50°C.
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Jo Shindo, Manabu Ueshin, Hidemasa Miki
2000 Volume 66 Issue 4 Pages
726-730
Published: July 15, 2000
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Since equilibrium water content (We) was considered to decrease with the denaturation of fish muscle protein, critical water contents (Wc), which were obtained on the characteristic dehydration curve during dehydration with a low osmotic sheet, were measured to investigate the relationships between the denaturation of myofibrillar (Mf) protein and Wc in tilapia surimi during storage for 0, 2, 4, 6, 8, and 10 days at 0°C. An inflection point was obtained in the falling rate period of logarithmic dehydration curve for the surimi, and it was named the second critical water content ((Wc)
2). The (Wc)
2 decreased with decreases in MfCa
2+-ATPase total activity and Mf solubility during storage. Positive high correlations between (Wc)
2 and MfCa
2+-ATPase total activity, and between (Wc)
2 and Mf solubility were obtained. As a result, (Wc)
2 could be useful as an index for evaluating protein denaturation.
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Akira Nomura, Yoshiaki Itoh, Koichi Yahata, Nariyuki Taniwaki, Atsushi ...
2000 Volume 66 Issue 4 Pages
731-736
Published: July 15, 2000
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A myosin heavy chain degradation inhibitor (MDI) prepared from the sarcoplasmic protein of a particular fish meat inhibited the serine protease (trypsin, chymotrypsin) activity. The degradation of myosin heavy chain in the washed meat around 40°C was also restrained by adding serine protease inhibitors. It is therefore conceivable that MDI is water soluble and acts as an inhibitor against serine protease in washed meat. MDI was isolated from the water soluble fraction of fish meat through a 4-step process, batch type DEAE ion exchange, ammonium sulfate fractionation, DEAE ionexchange chromatography, and Sephacryl S-300 gel filtration chromatography. It was found to be a monomeric protein with a molecular weight of 80, 000.
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Masao Ohno, Akinori Dan, Masanori Hiraoka, Hiroshi Nabeshima
2000 Volume 66 Issue 4 Pages
737-738
Published: July 15, 2000
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Goro Yoshida, Koji Yoshikawa, Toshinobu Terawaki
2000 Volume 66 Issue 4 Pages
739-740
Published: July 15, 2000
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Hajime Yasui
2000 Volume 66 Issue 4 Pages
742-743
Published: July 15, 2000
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Nobuyoshi Nanba
2000 Volume 66 Issue 4 Pages
744-745
Published: July 15, 2000
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Goro Yoshida
2000 Volume 66 Issue 4 Pages
746-747
Published: July 15, 2000
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Masao Ohno
2000 Volume 66 Issue 4 Pages
748-749
Published: July 15, 2000
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Yoshikazu Fujikawa, Shinji Kirihara
2000 Volume 66 Issue 4 Pages
750-751
Published: July 15, 2000
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Shinji Kirihara, Yoshikazu Fujikawa
2000 Volume 66 Issue 4 Pages
752-753
Published: July 15, 2000
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Noboru Murase
2000 Volume 66 Issue 4 Pages
754-755
Published: July 15, 2000
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Miyuki Maegawa
2000 Volume 66 Issue 4 Pages
756-757
Published: July 15, 2000
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Yasushi Nakajima
2000 Volume 66 Issue 4 Pages
758-759
Published: July 15, 2000
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Koji Yoshikawa
2000 Volume 66 Issue 4 Pages
760-761
Published: July 15, 2000
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