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NARIHARU YAMASHITA
2003 Volume 69 Issue 5 Pages
693-696
Published: September 15, 2003
Released on J-STAGE: February 01, 2008
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TADAHISA SEIKAI
2003 Volume 69 Issue 5 Pages
697-700
Published: September 15, 2003
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KATSUYUKI HAMASAKI
2003 Volume 69 Issue 5 Pages
701-704
Published: September 15, 2003
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KENICHI KAWASAKI, YASUHIRO FUNATSU
2003 Volume 69 Issue 5 Pages
705-708
Published: September 15, 2003
Released on J-STAGE: February 01, 2008
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YASUJI MASUDA, TAKAFUMI SAKO, GOU MATSUSHITA, TETSURO SHIRAISHI, JUNIC ...
2003 Volume 69 Issue 5 Pages
709-716,852
Published: September 15, 2003
Released on J-STAGE: February 01, 2008
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The ages of brown barracuda Sphyraena pinguis sampled from Kagoshima Bay, southern Japan were determined from transverse sections of otoliths. The following two methods were used for age validation: (1) monthly change in marginal growth rate for 1574 sectioned otoliths sampled from the waters, and (2) monitoring of strong or weak year classes during five survey years. The former method revealed that one annulus was formed per year during spring to early summer months. The latter one also validated one annulus per year. Assuming a birth date of June 1 from the monthly change in gonadosomatic indices, ages were assigned to every individual according to the number of opaque zones, and then fitted to the von Bertalanffy growth equations. Equations obtained were L
t=304.6{1-exp[-0.433(t+3.385)]} for males, and L
t=337.5{1-exp[-0.421 (t+2.972)]} for females, where L
t is fork length (mm) at age t(year) . These two equations were significantly different (p<0.01) and the length of females calculated from the equation was larger than that of males at any age. Maximum age observed was 11 years for males and 8 years for females.
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MASAYA OKABE, SINGO SEKI, MASARU NISHIYAMA, HIDETOSHI KUWAHARA, AKIRA ...
2003 Volume 69 Issue 5 Pages
717-725,852
Published: September 15, 2003
Released on J-STAGE: February 01, 2008
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Temperature adaptability was compared among three different races of ayu Plecoglossus altivelis at 205-220 days of age by recording changes in their tolerance temperatures after acclimation to various temperatures. These races were raised under the same conditions from hatching up to the performance of temperature-tolerance tests to make environment factors uniform. Tolerance temperatures of the fish changed depending on the acclimation temperature within the race and there were significant differences in the indices of temperature tolerance among the races. Average critical thermal maximum and minimum acclimated to 15-23°C and ranged from 29.7-32.2°C, 2.5-6.0°C in amphidromous form, 28.7-31.7°C, 2.3-5.3°C in landlocked form and 29.7-33.0°C, 1.7-5.0°C in Setogawa artificial landlocked form. The magnitude of thermal tolerance zones for each race calculated from the upper and lower incipient lethal temperatures against acclimation temperatures were 481°C
2, 415°C
2 and 517°C
2 respectively. These results suggested that a difference may exist in the temperature adaptability among the ayu races.
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TAKASHI YANAGIMOTO
2003 Volume 69 Issue 5 Pages
726-732,852
Published: September 15, 2003
Released on J-STAGE: February 01, 2008
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A method of identifying seven greenling species of the Hexagrammidae distributed around Japan has been developed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Nucleotide sequences of mitochondrial 12S rRNA-16S rRNA gene from seven greenling species were determined using PCR amplicons from genomic DNA extracted. Species-specific sites for three restriction enzymes (DdeI, DpnII, and MspI) were found, allowing development of a diagnostic PCR-RFLP protocol for identification of the seven greenling species.
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OSAMU KATANO, TOMOYUKI NAKAMURA, SHOICHIRO YAMAMOTO
2003 Volume 69 Issue 5 Pages
733-737,852
Published: September 15, 2003
Released on J-STAGE: February 01, 2008
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Number and maximum standard length (SL) of Japanese minnow consumed by bluegill were investigated in aquaria. Fifteen live Japanese minnow (2.5-7.9 cm initial SL) were released into each aquarium with one bluegill (5.7-14.3 cm SL) to investigate the consumption of Japanese minnow by bluegill during ten days. All bluegill except that of 5.7 cm SL consumed Japanese minnow. The maximum length and the total weight of Japanese minnow preyed upon by bluegill were correlated with SL of bluegill; bluegill consumed 5.8 g (5.6% of initial bluegill weight) of Japanese minnow per day at the maximum.
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SUZU SAKAO, TAKAFUMI FUJIMOTO, MINORU TANAKA, ETSURO YAMAHA, KATSUTOSH ...
2003 Volume 69 Issue 5 Pages
738-748,852
Published: September 15, 2003
Released on J-STAGE: February 01, 2008
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Although tetraploid is important for the mass production of sterile triploids by hybridizing with diploid, its survival capacity is extremely low. In this study, we aimed to identify the cause of such a high mortality of embryos treated with hydrostatic pressure shock (700 kg/cm
2, 7 min duration, 5-7 h after fertilization at 10°C in masu salmon. In treated eggs, cleavage rates were delayed and aberrant cell divisions were observed during the early cleavage stage. Histological observation in the blastula stage revealed frequent occurrence of aberrant blastoderms including anuclear and/or small blastomeres. Ploidy analysis of eyed and hatching embryos revealed successful production of pure tetraploid individuals. However tetraploid survivors exhibited abnormal appearances. In the groups treated at 6.5 and 7 h, no embryonic body was observed at hatching stage. Judging from the results of each treated group, the optimum timing for the inhibition of first cleavage was 6 h after fertilization, when the eggs were cytologically staged as prometaphase to metaphase of the first cleavage . Aneuploids and mosaics were also detected in treated embryos by flow cytometry. These results suggest that abnormal cleavage and blastomeres mosaicism caused by the treatment at the first cleavage give rise to high mortality.
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YING LIU, KAZUMI SAKURAMOTO, TAKERU KITAHARA, NAOKI SUZUKI
2003 Volume 69 Issue 5 Pages
749-756,852
Published: September 15, 2003
Released on J-STAGE: February 01, 2008
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We studied the scoop net fishery for ayu in Lake Biwa. We investigated the fishery management problem when the engine power of fishing boats is classified into three classes. The equilibrium net profit was obtained as the solution of a non-cooperative dynamic game. We investigated the behavior of the solution using simulations. When the initial population size was lower, the lowest class was profitable. However, the highest classes became profitable, as the initial population size increased. The total net profit for middle class fishermen over ten years, calculated based on estimated initial population sizes, was the most profitable. The total net profit decreased as the number of fishermen in the higher class increased.
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HIROYUKI MATSUNARI, TOSHIO TAKEUCHI, YUKO MURATA, MAKOTO TAKAHASHI, NO ...
2003 Volume 69 Issue 5 Pages
757-762,853
Published: September 15, 2003
Released on J-STAGE: February 01, 2008
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This experiment was conducted to investigate the changes in taurine content during the development of artificially produced yellowtail Seriola quinqeradiata larvae and juveniles. Besides this, the taurine content of wild caught and reared yellowtail was compared. We analyzed the free amino acids content in larvae and juveniles produced at the Goto Station of Japan Sea-Farming Association and Nagasaki Prefecture Institute of Fisheries (TL, 4.2-186.8 mm) and juveniles caught from the coastal area of Nagasaki Prefecture (TL, 29.7-179.0 mm). The content of most free amino acids such as lysine, Ieucine, isoleucine, alanine decreased between the fertilized egg stage and open-mouth stage, with the exception of taurine in artificially reared fish. The taurine content in the whole body of the artificially reared larvae decreased rapidly during the rotifer feeding. The amount of taurine in the wild fish was higher compared to the cultured fish. These results reveal that taurine has an important role compared to other free amino acids, suggesting live food and diet used for yellowtail culture would not satisfy the taurine requirement of yellowtail.
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DUSIT THANAPATAY, KENKICHI FUKUROTANI
2003 Volume 69 Issue 5 Pages
763-769,853
Published: September 15, 2003
Released on J-STAGE: February 01, 2008
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We measured the relative spectral sensitivities of monophasic and biphasic S-potentials in the light-adapted retina of the Japanese flounder, and compared them with spectral absorbances and sensitivities of green and blue cones, respectively. Furthermore, we found that the delay time of the depolarizing response of biphasic S-potential was longer than that of the hyperpolarizing responses of monophasic and biphasic S-potentials. Histologically, we found that the cone mosaic of the Japanese flounder is square and that it has no any accessory cones. We discuss the cone visual pigment of the Japanese flounder as well as the neural circuits of the cones and horizontal cells based on the above results.
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YUJI KURIYAMA, TADASHI TOKAI, KENJI TABATA, HARUYUKI KANEHIRO
2003 Volume 69 Issue 5 Pages
770-781,853
Published: September 15, 2003
Released on J-STAGE: February 01, 2008
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Litter amounts and composition on the seabed of Tokyo Bay were investigated during 1995-2000. More than 30 different types of litter items were collected and the most abundant were plastics and metals, accounting for about 90% of the total. The amounts of litter decreased from 338 items/km
2 (20.1 kg/km
2 in weight) in 1995 to 185 items/km
2 (10.4 kg/km
2 in weight) in 2000. The decreasing rate during 1995 to 2000 was 45.3% in number and 48.3% in weight. This reduced trend of litter amounts during the recent six years may reflect the reduction of discarded litter in our daily life and into the sea and the removal of litter by bottom trawl fishing boats. The production year of beverage cans collected by trawl surveys during 1996-2000 were examined and age analysis of cans by the Virtual Population Analysis (VPA) method was carried out. The value of the survival rate of the beverage can in the sea estimated by the VPA method was 0.47 for alurminum cans and 0.38 for steel cans.
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TAKESHI KOBAYASHI, BON KIMURA, TATEO FUJII
2003 Volume 69 Issue 5 Pages
782-786,853
Published: September 15, 2003
Released on J-STAGE: February 01, 2008
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We investigated the possibility of microorganisms contributing to reduced toxicity of ovary from puffer fish. Puffer fish ovary is fermented in salted rice-bran paste in Ishikawa Prefecture, Japan, and is one of the special marine products of this district, though fresh ovary seems to be tainted by puffer fish toxin, tetrodotoxin. A portion of liquid was taken from a vessel of rice-bran paste during the manufacturing process and known amounts of tetrodotoxin were added and their toxicities were measured. Also, 185 isolates from the products during the process of manufacturing were incubated in broth media containing tetrodotoxin and toxicity was measured after two-weeks incubation. The products were directly added to a medium containing tetrodotoxin, and toxicity and microorganisms were also investigated. A distinct toxicity decrease originating from the rice-bran-paste microorganism was not confirmed. On the other hand, the mouse bioassay method of evaluating the toxicity of known amounts of tetrodotoxin added in the fermented puffer fish ovary showed low recoveries (50-75%) of added amounts.
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YASUYUKI TSUKAMASA, TOMOKAZU HAGIHARA, MASASHI ANDO, YASUO MAKINODAN, ...
2003 Volume 69 Issue 5 Pages
787-795,853
Published: September 15, 2003
Released on J-STAGE: February 01, 2008
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We characterized the physical properties of commercial kamaboko, produced in various areas in Japan, by using a puncture test with 12 physical parameters. When measurement was conducted with a discoidal plunger of 0.3 cm diameter, the distance of 0.7 cm was required to avoid the effect of adjoining measurement marks. The effect of position for the measurement on the physical properties of the sample was studied. The vertical positions had a significant effect on the many physical parameters for all of the samples. However, the positions showing the highest physical properties were different among samples. On the other hand, samples of sliced pieces and horizontal positions for the measurement produced the effect for some of samples. The principal component analysis of the data demonstrated that the first component explained the general physical property of kamaboko at the breaking point in the puncture test, and the second component explained the physical property of kamaboko during the breaking process.
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YOSHINOBU HIRAOKA, TADAAKI KAN, MIKA KURONO, KAZUE HIRANO, HIROSHI MAT ...
2003 Volume 69 Issue 5 Pages
796-804,853
Published: September 15, 2003
Released on J-STAGE: February 01, 2008
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The freshness and kamaboko gel-forming ability of shortfin lizard fish Saurida elongata meat rapidly deteriorate during cold storage at 5°C. We found that fish meat stored in that manner contains a large quantity of trimethylamine-N-oxide (TMAO) above 25 mmol/kg. TMAO rapidly degrades into formaldehyde and dimethylamine. The amount of formaldehyde increased to high levels at which the kamaboko gel-forming ability was almost lost after 2-3 days' of storage. Shortfin lizard fish meat produced formaldehyde abundantly at the setting temperature of 30°C for 6 hours. Washing with water effectively removed TMAO from minced meat. Deterioration in gelforming ability was reproduced by addition of formaldehyde to washed shortfin lizard fish meat. In contrast, white croaker Argyrosomus argentatus meat contained over 50 mmol/kg of TMAO, but it retained its gel-forming ability well during storage because of its lack of formaldehyde. Adding formaldehyde to washed6 white croaker meat and shortfin lizard fish meat decreased gel-forming ability.
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MOTOHIDE TOCHINO, MASAYUKI YAMAMOTO, KENICHI YAMAGA, MUNEHIRO FUJIWARA
2003 Volume 69 Issue 5 Pages
805-807,853
Published: September 15, 2003
Released on J-STAGE: February 01, 2008
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SETSUKO IWANAGA
2003 Volume 69 Issue 5 Pages
825-826
Published: September 15, 2003
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HIROSHI NAGAI
2003 Volume 69 Issue 5 Pages
827-828
Published: September 15, 2003
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NAOMASA OSHIRO
2003 Volume 69 Issue 5 Pages
829-830
Published: September 15, 2003
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KAZUO SHIOMI
2003 Volume 69 Issue 5 Pages
831-832
Published: September 15, 2003
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HIDEYUKI NAKAGAWA
2003 Volume 69 Issue 5 Pages
833-834
Published: September 15, 2003
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