Decoloration of Orange II catalyzed horseradish peroxidase (HRP) with activator as
p-iodophenol, sodium nonanoyloxybenzenesulphonate (NOBS) and sodium
p-phenolsufonate under low temperature was studied kinetically. Decoloration rate constant (
k) that can prevent dye transfer of Orange II (
k = 2.0 min
-1) was not obtained in 3.4 × 10
-8 M HRP without activator. Optimum condition using
p-iodophenol was pH 8.9 Carmody buffer and 5.0 × 10
-5 M
p-iodophenol. Optimum pH using NOBS and sodium
p-phenolsufonate were 10.1 and 10.3 respectively. Decoloration rate constant decreased with increase in the concentration NOBS.
k = 2.0 min
-1 was not reached unless high sodium
p-phenolsulfonate. Therefore, it was found that the use of
p-iodophenol appears optimal.
Decoloration reaction of Orange II using HRP with
p-iodophenol and sodium percarbonate to prevent dye transfer was optimized under low temperature. The activation energy of decoloration of Orange II using HRP with
p-iodophenol was 1/2 - 1/3 of those without activator and using sodium percarbonate.
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