Japanese Journal of Allergology

Antibody producing potency of patients with bronchial asthma, rheumatoid arthritis or other collagen diseases were investigated concerning ABO blood group isoagglutinis and skin sensitizing antibodies. The isoagglutinin levels of these patients were, as a whole, almost equivalent to those of controls. There was also no significant difference among these groups in elevation of the isoagglutinin titers after stimulation by subcutaneous injection of mismatched blood. A relatively weak response to the stimulation was observed in the patients with collagen diseases including 2 patients with S.L.E.. Positive skin reactions to house dust and ragweed extracts, were more frequently observed in the asthmatic group and, on the contrary, less frequently in the rheumatoid group than in the control group. But there was no such marked difference among them in skin reaction with monilia extract. Fifty-two subjects consisting of asthmatic, rheumatoid, and control patients received weekly intramuscular injections of ACTH-Zn preparation. Using aquous solution of an ACTH preparation at the concentration of 0.002 units/ml., it was proved that atopic asthmatic patients earlier and more frequently, and rheumatoid patients less frequently developed positive turn of the immediate skin reaction than the control group. The intensity of skin reactions to histamine and acetylcholine proved almost similar in three these groups, except that the threshold of skin reaction to acetylcholine was lower in rheumatoid patients.

View full abstract

Show abstractHide abstract

Na-Cu-cholorophyllin (Cu-C) has been reported by Sindo et al. to be a strong complement inhibitor. Nishioka et al. have found that the reactivity of C'3b was blocked with Cu-C. Effect of Cu-C on the homograft reaction that is considered to belong to the delayed type reaction was studied. 1)Skin grafts of CFW mice transplanted in the homologous C3H/He mice survived statistically longer than controls when the recipients, C3H/He mice, were treated with intraperitoneal injections of Cu-C, 1.0mg/10gm/day, for 8 days. The 2nd-set phenomenon in this system was completely inhibited with local injections of Cu-C, 0.25mg/10gm/day, for 7 days. 2)MH134 tumor cells native in C3H/He strain inoculated intraperitoneally in the homolous C57B1 mice progressed markedly when the recipients were injected every day with Cu.C, 1.0mg/10gm/day, although the hosts resisted for a while approximate 7 days after the tumor inoculation. Thus the homograft reaction against ascites tumor was so markedly inhibited with Cu-C that the recipients died from the progressed tumor within 13 days. On the contrary, homograft reaction to MH134 tumor was accelerated when the recipients, C57B1 mice, were treated with less than approximate 0.1mg/10gm/day of Cu-C. From these results it was suggested that homograft reaction is inhibited with Cu-C markedly when target graft cells sensitized with antibody contact with concentrated Cu-C. The observations that Cu-C of the ranges of dosis employed sohwed rather stimulated antibody production to both sheep erythrocytes and tumor homograft in mice without severe toxic effects suggest that Cu-C dose not effect in the inhibition of antibody production but blocks complement reaction that causes destruction of the sensitized graft cells.

View full abstract

Download PDF (953K)

The intraperitoneal cells of sensitized guinea pigs were studied on their antigen-carrying properties. In the first report the results of the investigation on the effect of the injected with a suspension of heat-killed tubercle bacilli in paraffin-oil were presented. 1) Tuberculin reaction began to be positive from the second week after the sensitization. It became more marked on the third week, increased more and more the intensity thereafter and reached almost constant intensity between the fifth week. 2) The white cell count of peripheral blood was increased after the sensitization and the absolute number of the lymphocytes was also markedly increased. In the intraperitoneal cells, increase in both large mononuclears and lymphocytes was noted after the sensitization. 3) Increase in the intraperitoneal cells, especially large mononuclears by intraperitoneal injection of 0.05 % glycogen in physiological saline was noted in sensitized guinea pigs and it reached the peak on the fourth day after the injection. 4) The India ink phagocytosis of the intraperitoneal cells in sensitized guinea pigs was markedly increased when OT was added in the India ink.

View full abstract

Register with J-STAGE for free!