Influences of the anabolic sreroids on the production of antibodies and allergic responses in animals were studied and following results were obtained. 1. Rabbit were sensitized with egg-albumin and treated simultaneously with various anabolic steroids. (HMD, NTPP, Δ, 17MT and 4Cl-TA). Precipitating antibody titer and agglutination titer of the anti-egg-albumin serum was measured by the clinical test tube method and Boyden's method. Antibody titers in a group of rabbit which received anabolic ateroids were enhanced about 4-8 times compared with that in control group. It is likely that doses and method of treatment with anabolic steroids gave influences on production of antibodies in this experiment. 2. The rate of decrease in precipitating antibody titer after immunization was rather retarded in sera of rabbit treated with anabolic steroids compared with the rate in untreated rabbit. 3. Influence of anabolic steroids on anaphylactic shock induced in mice sensitized with horse serum. a) Minimum dose of antigen necessary to provoke active anaphylactic shock in mice showed no marked change by the use of anabolic steroids, but the mortality in the with anabolic steroids treated group was less than that of the untreated group. b) The degree of anaphylactic shock induced in mice had some connection with doses of anabolic steroids. c) In the induced anaphylactic shock in mice, mortality was increased in the group treated with both anabolic steroids and corticosteroid (Prednisolone). On the contrary, it decreased particularly in the group treated with HMD alone. d) Provocation of passive anaphylaxis in mice proved difficult. 4. Influence of anabolic steroids on anaphylactic shock induced in guinea pig. a) It was revealed that none of the anabolic steroids could inhibit or alleviate active anaphylactic shock. b) Concerning the passive anaphylactic shock induced in guinea pigs, the mortality rates in HMD treated group, with horse serum treated group, HMD and horse serum administered group, and in control group were 46%, 46%, 7.7% and 40% respectively. The score of symptoms of anaphylactic shock in group given both HMD and horse serum was extremely low. 5. Influence of anabolic steroids on passive cutaneous anaphylaxis^* induced in guinea pigs. (^*PCA) Following the original method by Ovary, PCA was induced in guinea pigs with an anti-horse rabbit serum. Anabolic steroids (HMD, Δ, 17MT, 4Cl-TA, and NTPP) and prednisolone were used in this experiment, and following results were obtained. a) The effects of anabolic steroids on PCA reaction proved beneficial. Similar effect of prednisolone was also observed. b) The PCA reaction in the group receiving anabolic steroids in combination with serum of other species differed from that in the control group, but the degree of inhibitory effect was similar in both anabolic steroids treated group and in with foreign protein treated group. 6. Influence of anabolic steroids and prednisoloneon the ratio; PCA antibody titer/precipitating antibody titer. Parallel with the production of precipitating antibody, production of the PCA antibody titer was increased, and the ratio of these antibodies was not different between the HMD treated group and control group. But the ratio in with prednisolone treated group was lower than that in other groups. 7. Influence of anabolic steroid alone on anti-hyaluronidase activity. The anti-hyaluronidase activity in rabbit was likely to increase by the anabolic steroids. Therefore, anabolic steroids as well as corticosteroid and ACTH seems to enhance the anti-hyaluronidase activity.

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Using fluorescent antibody technique, the distribution and amount of duck- and rabbit-gamma globulin in glomeruli of rabbits after injection of duck anti-rabbit kidney serum (NTS) were examined for periods up to 210 days. In all experiments, nephrotoxic serum nephritis was induced by a single intravenous injection of 2.5 ml/Kg body weight of NTS. The animals were killed on 20th minute to 210th day following injection of NTS. Duck- and rabbit-gamma globulin in glomeruli were identified by staining following injection of NTS. Duck- and rabbit-gamma globulin in glomeruli were identified by staining sections of frozen renal tissues with fluorescein-labeled antibodies to duck- and rabbit-gamma globulin. Duck-gamma globulin was found to localize selectively in the glomerular basement membrane (BM) within 20 minutes following injection and there was no remarkable loss of its intensity until the end of four weeks, subsequently the amount of duck gamma globuoin in glomeruli decreased very slowlh and at the end of the experiment it was barely perceptible as a specific fluorescence in glomeruli. In contrast, rabbit-gamma globulin appeared in the glomerular BM 6 days after injection and its localization in the glomerular BM corresponded closely to that of duck-gamma globulin. Its intensity became maximum at 8 or 10 days and persisted for 210 days until the end of the experiment. In certain animals whicy had subacute nephritis, rabbit-gamma globulin was found in the part of crescent formation as well as in the capillary loop. No duck-gamma globuoin was recognized in the extracapillary parts of crescent formation. The significance of autollgous (rabbit) and heterologous (duck) gamma globulin localizing in glomeruli was discussed in relation to the pathogenesis and progression of nephrotoxic serum nephritis.

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House dust is one of the most important antigen for patients with respiratory allergy, but its constitution is very complexed and is considered not to be the same either in each house or in each place. In this report, the incidences of positive reaction of skin test with house dust antigen in various districts of Japan have been comparably studied and further the skin test with house dust antigen itself was studied in some detail giving the following results: 1. The incidences of positive reaction of skin test with house dust antigen in various districts of Japan were from 4% to 32% or 16% in average in the so-called healthy persons, from 31% to 100% or 65% in average in the patients with bronchial asthma, from 3% to 22% or 19% in average in the patients with chronic urticaria, 20% in average in the patients with oyster-shucker's asthma and 28% in the patients with other allergic disorders. 2. The relationship between the skin test with house dust antigen and the age of subjects examined was studied. In the so-called healthy persons, the incidence of the positive reaction of skin test with house dust antigen was seen already from the teens and showed its peak in the latter half of the twenties and the first half of the thirties and then gradually decreased, but even the seventies a positive reaction was still found. In the patients with bronchial asthma, there was a difference between the patients in Morioka and those in Hiroshima in the incidence of positive reaction of skin test with respect to the age of the patients, but on an average two peaks were seen in the incidence of positive reaction of skin test, that is one in the age period of from five to fourteen years and the other in the same of from thirty to thirty-four years of age. As to the relationship between the age of onset of bronchial asthma and the skin test with house dust antigen positive reaction was seen in a highincidence in patients whose onset of asthma was before fourteen years of age, and thereafter showed gradual decrease in its incidence with advance of age. 3. The incidence of positive reaction of skin test in asthmatics with house dust antigens extracted from house dusts collected in the houses of each asthmatic varied from 0% to 60%, the same house dust antigen showing even a difference between Morioka and Hiroshima in the result of the skin test. The incidence of positive reaction of skin test with patient's own house dust antigen was high and there were some patients who reacted only with their own house dust antigens. 4. In three hundred and fourty-three patients with bronchial asthma, the incidence of positive reaction of skin test with all the antigens examined was 64%, of which 52% were with house dust antigen. Seeing these skin tests from viewpoint of age of the asthmatics, an incidence of positive reaction with antigens other than house dust was slightly high in the patients from the teens to the thirties and from the viewpoint of age of onset of asthma it showed a high peak in the latter half of the teens of asthmatics which was noted not to be parallel to the incidence of positive reaction with house dust antigen. 5. As to the relationship between the skin test with house dust antigen and hereditary disposition, while asthmatics who had the disposition showed a high incidence of positive reaction, asthmatics without the disposition demonstrated no remarkable difference between the positive and negative incidence of the reaction. 6. As to house dust antigen therewas a significant correlation between its skin test and Prausnitz-Kustner reaction, but no correlation was found between its skin test and leucocytolysis in vitro. 7. Regarding the relationship between the hyposensitization treatment and skin test with house dust antigen, the reaction of skin test became weak in most of the patients, in whom the hyposensitization treatment was favourable, but it did not show change in

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