Japanese Journal of Allergology
Online ISSN : 1347-7935
Print ISSN : 0021-4884
ISSN-L : 0021-4884
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Volume 31, Issue 2
Displaying 1-14 of 14 articles from this issue
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  • [title in Japanese]
    Article type: Cover
    1982 Volume 31 Issue 2 Pages Cover13-
    Published: February 28, 1982
    Released on J-STAGE: February 10, 2017
    DOIhttps://doi.org/10.15036/arerugi.31.Cover13
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    Article type: Cover
    1982 Volume 31 Issue 2 Pages Cover14-
    Published: February 28, 1982
    Released on J-STAGE: February 10, 2017
    DOIhttps://doi.org/10.15036/arerugi.31.Cover14
    JOURNAL FREE ACCESS
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  • [title in Japanese]
    Article type: Bibliography
    1982 Volume 31 Issue 2 Pages Misc5-
    Published: February 28, 1982
    Released on J-STAGE: February 10, 2017
    DOIhttps://doi.org/10.15036/arerugi.31.Misc5
    JOURNAL FREE ACCESS
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  • AUGMENTATION OF LYMPHOCYTE TRANSFORMATION BY HUMAN DIALIZABLE TRANSFER FACTOR AND ITS TARGET CELL
    Masahiko Okada
    Article type: Article
    1982 Volume 31 Issue 2 Pages 69-80
    Published: February 28, 1982
    Released on J-STAGE: February 10, 2017
    DOIhttps://doi.org/10.15036/arerugi.31.69
    JOURNAL FREE ACCESS
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    Investigations have been made to characterize transfer factor (TF) activities in vivo and in vitro. Although lymphocyte transformation (LT) is a useful in vitro assay systems for evaluating TF activities, there is unanimous agreement about the methods and results. In this report, the effects of TF on LT, and especially of T cells were studies. T cells were obtained by depletion of adherent cells from heparinized peripheral blood mononuclear cells using plastic dishes and nylon wool columns. The stable LT augmenting effects of TF were always seen, where T cells were preincubated for 60 min with TF before the culturing with PHA or Con A. The LT in the combination of T cells and preincubated adherent cells with TF was not augmented compared with the control. These data suggest that the T cells were the target cells of TF activity in LT. The effects of TF fractions on T cells were also studied. The fractions (I-VI) were obtained from TF by Sephadex G-25 gel filtration. The fraction V had a significant augmenting effect on T cell response in LT. Glycine (68.5%), tyrosine (5.0%), serine(4.7%), and glutamic acid (4.0%) were the major components of amino acids of fraction V after hydrolysis of TF. The results of chemical analysis using spectrophotometer, thin-layer chromotography and fluorescamin staining indicated that fraction V contained some peptides.
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  • DETECTION OF SPECIFIC IgE ANTIBODIES BY PHADEZYM RAST^[○!R] : A Newly Developed Kit Using the Method of Enzyme-Linked Immunosorbent Assay(ELISA)
    Keisuke Shinomiya
    Article type: Article
    1982 Volume 31 Issue 2 Pages 81-85
    Published: February 28, 1982
    Released on J-STAGE: February 10, 2017
    DOIhttps://doi.org/10.15036/arerugi.31.81
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    Phadezym RAST^[○!R] is a newly developed kit for an in vitro immunoassay to detect circulating specific IgE antibodies by an enzyme-linked immunosorbent assay (ELISA). The working principle of the kit is identical with that of Phadebas RAST^[○!R]. The reactions take place between the specific IgE antibodies in the test sample and the coupled antigen on the paper disc. After the reaction, the specific IgE antibodies are detected by a specific antibody against human IgE (Dε2) labelled with a marker enzyme. The evaluation of this kit at this time is focused on the correlation between the results with this kit and with Pharmacia RAST^[○!R] and the reproducibility of the results. Test sample sera were collected randomly from asthmatic children on the basis of the results of skin tests. The correlation between the results of skin tests and Phadezym RAST^[○!R] was not so good as with Phadebas RAST^[○!R]. However, the results with this kit and Phadebas RAST^[○!R] correlated well (r=0.958, p<0.001). The effect of the incubation time in the third incubation where the reaction between the enzyme labelled on the anti IgE antibody and the substrate proved that two-hour of incubation at 37℃ is most appropriate. To maintain reproducibility of results, it is important to keep the incubation time accurately in the third incubation. It was proved that the color change induced by the enzymatic reaction was stable overnight at room temperature after the addition of the stop solution.
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  • EFFECT OF NOCARDIA RUBRA CELL WALL SKELETON ON CULTURED SPONTANTANEOUS CELL MEDIATED CYTOTOXICITY
    Hiromitsu Matsuzaki, Fumio Kawano, Koichi Araki, Susumu Kishimoto
    Article type: Article
    1982 Volume 31 Issue 2 Pages 86-93
    Published: February 28, 1982
    Released on J-STAGE: February 10, 2017
    DOIhttps://doi.org/10.15036/arerugi.31.86
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    [in Japanese]
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  • ELECTRON MICROSCOPIC STUDY OF MAST CELLS IN ALLERGIC NASAL INTEREPITHELIAL SPACE AND LAMINA PROPRIA : Before and After Provocation
    Shinichi Kawabori, Tokuzi Unno, Mitsuaki Takahashi, Minoru Okuda
    Article type: Article
    1982 Volume 31 Issue 2 Pages 94-99
    Published: February 28, 1982
    Released on J-STAGE: February 10, 2017
    DOIhttps://doi.org/10.15036/arerugi.31.94
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    Observation of mast cells in subjects with nasal allergy was done by electron microscopy before and after provocation in 3 areas; the nasal interepithelial space, the subepithelial layers and the deep layers of the nasal lamina propria. The following results were obtained. 1.The amount of degranulation of the mast cells in the nasal interepithelial space and the subepithelial layer increased after provocation. 2.The amount of degranulation of the mast cells in the deep layer of the nasal lamina propria was the same before and after provocation. 3.The main features of exchanged granules of the mast cells after provocation were a)swelling or lower electron density of the area enclosed by perigranular membrane, b)fibrillar and reticular changes of granular substance and c)the disappearance of the granular substance. It was, therefore, judged that mast cells in the nasal interepithelial space and in the subepithelial layer play a more important part in the onset of a nasal allergic reaction than do those in the deep layer of the nasal lamina propria.
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  • CHARACTERIZATION OF LYMPHOCYTIC CELLS INFILTRATING INTO THE FOLLICULAR EPITHELIAL LINING IN CHRONIC THYROIDITIS IN HUMANS
    Tomio Kotani, Katsutoshi Komuro, Takashi Yoshiki, Tetsuo Itoh
    Article type: Article
    1982 Volume 31 Issue 2 Pages 100-105
    Published: February 28, 1982
    Released on J-STAGE: February 10, 2017
    DOIhttps://doi.org/10.15036/arerugi.31.100
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    Lymphocytic cells infiltrating into the follicular epithelial lining were studied with immunofluorescence and electron microscopy to understand a mechanism destructive of thyroid tissue in chronic thyroiditis in humans. Medium to large T cells infiltrated first, followed by plasma cells with progress of thyroid lesion. T cells infiltrating into the follicular epithelial lining consisted of two types of cells, medium to large T cells with rich cytoplasm and small T cells with poor cytoplasm. The former were found more frequently than the latter and were observed at a very early stage in chronic thyroiditis. Furthermore, it was found that degeneration of follicular epithelium had already begun when the former cells infiltrated into the follicular epithelial lining. Medium to large T cells had been thought to be important in the destruction of follicular epithelial cells. According to our study, T cells seem to play a causal role in the destruction of follicular epithelial cells.
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  • ESTIMATION OF SPECIFIC IgE AND IgG ANTIBODIES TO EGG WHITE, OVALBUMIN AND OVOMUCOID IN HEN'S EGG ALLERGY
    Tokuko Mukoyama, Eisaku Iwasaki, Minoru Baba, Yoshihisa Hayashi, Masah ...
    Article type: Article
    1982 Volume 31 Issue 2 Pages 106-113
    Published: February 28, 1982
    Released on J-STAGE: February 10, 2017
    DOIhttps://doi.org/10.15036/arerugi.31.106
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    On thirty one children with hen's egg allergy specific IgE antibody and specific IgG antibody were investigated using egg white, ovalbumin and ovomucoid. Specific IgE antibody activity was determined by the radioallergosorbent test (RAST) and specific IgG antibody activity was measured by a method based on the binding of IgG antibody to Sepharose-coated staphylococcus protein A. The children with immediate type reaction showed high titer of specific IgE antibody and IgG antibody to egg white. The children with delayed type reaction showed high titer of specific IgG antibody to egg white, but poor reaction in skin testing and low titer of IgE antibody. Specific IgE antibody and IgG antibody to ovalbumin and ovomucoid were correlated well to egg white antibody. IgE antibody and IgG antibody showed higher titer in younger children.
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  • A COMPARATIVE PATHOPHYSIOLOGICAL STUDY OF BRONCHIAL ASTHMA PROVOKED BY INHALATION OF HISTAMINE OR ACETYLCHOLINE WITH SPECIAL REFERENCE TO DYNAMICS OF HISTAMINE
    Shun-ichi Kitazawa
    Article type: Article
    1982 Volume 31 Issue 2 Pages 114-124
    Published: February 28, 1982
    Released on J-STAGE: February 10, 2017
    DOIhttps://doi.org/10.15036/arerugi.31.114
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    Asthmatic attack was provoked by inhalation of histamine and/or acetylcholine in 27 patients (a total of 42 times) with bronchial asthma. During these attacks induced by the two different inhalants, changes in pulmonary function, histamine levels of plasma and whole blood, peripheral basophil and eosinophil counts and plasma levels of cyclic nucleotides were measured. The results were as follows: 1.Changes in the parameters of pulmonary function (FVC, FEV_<1.0>, peak flow rate, V50, V25) during histamine-provoked asthmatic attack was quite similar to that of acetylcholine provokation. 2.Attacks caused by either of two inhalants were not associated with remarkable changes in plasma histamine level, peripheral basophil and eosinophil counts and plasma cyclic nucleotides. 3.The elevation of histamine levels of whole blood were found in the histamine-induced attack, whereas this was not observed in the acetylcholine induced attack. 4.The elevated histamine level in whole blood after histamine provokation was inversely proportional to the concentration of inhaled histamine.
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  • PRINCE MELON ALLERGY
    Kaoru Tosaka, Keisuke Masuyama, Takeru Ishikawa, Kosuke Oka
    Article type: Article
    1982 Volume 31 Issue 2 Pages 125-133
    Published: February 28, 1982
    Released on J-STAGE: February 10, 2017
    DOIhttps://doi.org/10.15036/arerugi.31.125
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    The, "Prince Melon", a sort of honey dew, is intensively cultivated in the Kumamoto district. We found a patient who was involved in the cultivations of the melons and who had suffered from asthmatic attacks every spring season for 7 years. The season when the attacks took place was coincident with the melon-harvest time but not the flowering season. Antigen extraction was performed from the hair-like substances of the melon skin. Skin test threshold of the patient was 10^<-10> dilution. Provocation test was positive. RAST value of the patient's serum was extremely high, and the value was markedly lowered by preincubation of the serum with uncoupled original antigen. Rabbit antiserum to the melon extract showed 2 precipitating lines to the antigen by agar immunodiffusion. Results of routine skin test including the melon to all the other patients who visited our clinic indicated relative coincidence of the positive skin test to the melon and mite antigen. However, by the melon RAST inhibition test and agar immunodiffusion test with the mite, no inhibition or cross-reactivity was observed. The results show that the asthmatic attacks were provoked by Type I allergy of inhaled melon skin substance. Cross-reactivity among the "Prince Melon", watermelon, and muskmelon was observed by agar immunodiffusion, skin test and RAST inhibition test.
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  • [title in Japanese]
    Article type: Appendix
    1982 Volume 31 Issue 2 Pages 135-
    Published: February 28, 1982
    Released on J-STAGE: February 10, 2017
    DOIhttps://doi.org/10.15036/arerugi.31.135
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  • [title in Japanese]
    Article type: Appendix
    1982 Volume 31 Issue 2 Pages 137-140
    Published: February 28, 1982
    Released on J-STAGE: February 10, 2017
    DOIhttps://doi.org/10.15036/arerugi.31.137
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  • [title in Japanese]
    Article type: Cover
    1982 Volume 31 Issue 2 Pages Cover15-
    Published: February 28, 1982
    Released on J-STAGE: February 10, 2017
    DOIhttps://doi.org/10.15036/arerugi.31.Cover15
    JOURNAL FREE ACCESS
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