Japanese Journal of Allergology

Histamine is released from corpuscles of immunized subjects by the application of corresponding antigen. This experiment was aimed to antigen antibody reaction in immediate type allergy utilizing upper phenomenon. In vitro, to the blood from subjects with immediate type allergy corresponding antigen was added. And, released plasma histamine level was compared with by the case of antigen was added to the blood and by not. Plasma histamine level was determined by a modified fluorometric method devised by Noah and Brand. l) Patients with ACTH hypersensitivity (These patients showed positive cutaneous responses to ACTH by the methods of direct skin test and PK reaction): intense release of histamine was observed, but by the cases of normal subjects no release was observed. 2) Asthmatics; by the cases of patients with positive skin test and PK reaction with house dust intense release of histamine was observed, but the cases of patients with negative skin test and of normal subjects no release was observed. In some these normal subjects house dust was able to evoke positive cutaneous response and positive P-K reaction. 3) Subjects with positive cutaneous response to benzyl penicilloyl protein conjugate : by the case of simple hapten no release was observed, by the case of bivalent hapten weak release was observed and by the case of multivalent hapten intense release was observed. 4) Rabbits immunized with HSA, cephalothin and various penicillins: multivalent haptens were able to release histamine. From these experiments this "in vitro histamine release test" confirmed to be was useful to detect antigens and antibodies concerning immediate allergy or atopic disease.

View full abstract

Show abstractHide abstract

Effect of sodium auro-thiomalate preparation on bronchial asthma was investigated in co-operation with 10 medical schools and 2 general hospitals. It has been generally accepted that the effect of gold therapy in the treatment of bronchial asthma was not merely symptomatic but rather fundamental and over several hundred miligrams of gold used to be necessary to obtain an clinical improvement. The method of administration was as follows; initially 10 mg of the preparation of sodium auro-thiomalate was injected intramuscularily every week for 10 weeks, then the dosis increased to 25 mg, 50 mg and 100 mg in the same manner. Number of patients studied were 210 (male 125 and female 85) aging from 15 to 74. Daily changes in symptoms and other medications taken were recorded in asthma diary. Clinical effects were evaluated from the changes in the severity of asthmatic symptoms before and after the administration of sodium auro-thiomalate. The severity was estimated according to new criteria based upon the frequency and the intesity (degree of disturbance in A.D.L.) of asthmatic attacks. Overall effectiveness in these 210 patients was excellent in 33 patients (15.7%), good in 76 (36.2%), fair in 41 (19.5%), none in 46 (21.9%), and unclear in 14 (6.7%). Clinical effect among the patients who received more than 350 mg of sodium auro-thiomalate was excellent in 31 patients (18.6%), good in 68 (40.7%), fair in 30 (18.0%), none in 34 (20.4%), and unclear in 4 (2.4%). Among the patients received more than 850 mg, excellent result was obtained in 28 (23.3%), good in 45 (37.5%), fair in 23 (19.2%), and none in 24 (20%). And among the patients received more than 1850 mg, excellent result was seen in 14 (25%), good in 24 (42.9%), fair in 10 (17.9%), and none in 8 (12.8%). Thus the more the total dosage was increased, the better results were obtained. Side effects were observed in 40 patients. Major side effects were skin eruptions, stomatitis and albuminuria, as already reported with other kinds of gold preparation.

View full abstract

Download PDF (806K)

Anti-allergic and anti-inflamatory action of xylofuranoside derivatives were studied and the following results were obtained. l. No protective effect was observed in the cases of the severe passive anaphylaxis in the guinea pig (sensitized with about 8 times of antiserum required to produce fetal anaphylactic shock) by the pretreatment of the large amount of xylofuranoside derivatives; however, in the cases sensitized with antiserum just to produce fetal anaphylaxis a significant protective effect of the derivatives was observed. 2. The pretreatment of xylofuranoside to the passively sensitized mice seemed to be slightly effective against the fetal anaphylaxis in mice. 3. Xylofuranoside derivatives inhibited the in vitro contraction of the isolated guinea pig's ileum by the addition of histamine, acetylcholine, bradykinin and antigen; at the concentration of 100 mcg/ml of the derivatives, histamine, acetylcholine, bradykinin (0.1 mcg/ml) and anaphylactic contractions were completely inhibited and at the concentration of l0-30 mcg/ml incompletaly inhibited. 4. PCA reaction in the guinea pig was also slightly inhibited by the oral administration of the derivatives. 5. The inhibiting effect of the derivatives against the capillary and tissue permeability were also observed.

View full abstract

l. Serum from the rabbit sensitized with heat-killed tuberculous bacilli, showing highly elevated antibody titers, was injected into the right posterior lobe of the lung of guinea pigs and 4 hours later the antigen bacilli were intravenously injected in the same animals. 2. A large granuloma was formed in the injected lung lobe, 48 hours after the intravenous injection of antigen. 3. A similar lesion never took place in the control aterial lung into which normal rabbit serum had been injected as the control. 4. Accordingly, it seems likely that humoral antibodies against tuberculous bacilli have an important role on the formation of granulomatous lesions in the lung.

View full abstract

Register with J-STAGE for free!