Pyridoxal phosphate which is the activated type of Vitamin Be group in the living organism, functions as coenzyme in the process of various kinds of metabolism including that of amino acid, and attention is directed toward the relationship between the Vitamin B₆ group and the central nervous function.
For the purpose of determining Pyridoxal phosphate in the central cerebral tissue of guinea pigs, the author studied both methods; paperchromatographic technique and ultraviolet absorption band.
The investigation was made on the reproductivity of the estimation method within the scope of 0.1-5.0μg Pyridoxal phosphate per 1.0g cerebral tissue. By this method Pyridoxal phosphate was collected from the cerebral tissue at the rate of 81%.

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1) Thyroid uptake rate by radioactiveiodine (I¹³¹) on hyperthyroid patients reveals lower value at 24 hours rather than 3 hours. Regarding the therapy of the patients who have had treatment with antithyroid drugs, the measurement of half-life effective time of the ¹³¹I has to be performed. Particularly, it is desirable that a thorough observation be made on the patient's progress at the time of the 2nd treatment as well as the first time.
2) Measurement of half-life effective time of both the rate of uptake of thyroid and of therapy with radioactiveiodine were performend and the results were obtained as 2.6 days to 8 days, In case we measure the rate of thyroid uptake the same measurement was performed after a week in purpose to determine the correct treatment method.
3) Comparison was made to find the difference in the thyroid weight between scanning and palpation. The former indicated the larger difference, while in 14% of the total both showed equal value.
By scanning we could calculate the weight of the thyroid, which made us able to decide the doses of radiation of 6, 000 rad. And by comparing this doses with the doses clinically given, we obtained the average difference of 1mc. This difference seemed to have a big relationship to the desirable therapeutic dose.
In conclusion, for deciding adequate therapeutic doses we have to find the half-life effective time as well as to find the weight of the thyroid.

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In case of thoracic cavity operation closed drainage is well known as one of the most necessary postoperative treatments. For the purpose of closing dead space or removing excreta which are formed after surgical operation, closed drainage has excellent effects for shortening of the treatment periods.
Applying the method of the closed drainage the author had very satisfactory results for the treatment of mediastinal emphysema and bronchial fistula, which were believed to be hard to cure by the ordinary treatment.
Procedure and Result.
1. Mediastinal emphysema
When the patient is expected to get worse, from Fossa suprasternalis to mediastinum, the closed drainage should appiled immediately. If the patient is seriously ill and the surgical operations difficult to perform this method is more essential. Because we can expect effects by the closed drainage, we should not rely upon such a method as cutting the skin to have exhaust air from the lesion. The author has two unlucky cases both of which died of either mediastinal emphysema with pressure system or mediastinitis, although skin incisions were performed. While in one another case who was once serious, survived after 3 weeks by using closed drainage.
2. Bronchial fistula
Bronchial fistula after pulmonary resection is one of the most unpleasant complications. By various methods patients with bronchial fistula were treated with success. However, some fistulae were hard to close even though we made every effort for the closure.
In these cases we should always remember the application of the closed drainage, as well as care in the performance of the surgical operation.
Especially on the poor risk, we have to recognize the importance of this method. We have two cases of bronchial fistula, which at once seemed to be hard to cure, but applying the above said method they completely recovered.
3. Durable suction apparatus with a manometer which is a part of artificial pneumothorax apparatus.
The pressure in the thoracic cavity which makes us able to know the amount of air leakage, quantity of excreta and reexpansion of the residual lung, tells us when we are able to pull out the drain from the lesion. To do everything economically, and old artificial pneumothorax apparatus is set at the cirsuit of drain, then the index of the manometer will tell us when.

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In order to make clear the pathologic physiology of postoperative acute pulmonary edema, the author observed transudation and resorption of pulmonary hemodynamics as well as the flow of lung lymph, and the following results were obtained:
1) The flow of lymph obtained from truncus lymphaticus dexter at the normal condition was 0.01-0.06cc/sec., average 0.079cc/sec., and the lymph protein concentration was 2.0-4.9, average 3.23%.
2) The influence of lobectomy upon pulmonary lymph dynamics was hardly seen, and any remarkable changes of flow of lymph and protein concentration were not observed, and also change of pulmonary arterial blood pressure was seldom.
3) The flow of lung lymph and the pressure of arteria pulmonalis increased slightly by loading hypoxia and hypercapnia.
4) By the rapid intravenous injection of physiological saline solution, the flow of lung lymph increased so quickly and remarkably as seen in the pulmonary arterial blood pressure and the density of protein of the lymph fell down remarkebly.

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1) Although with or in Sauton agar medium it was not successful, using egg medium a streptomycin-dependent strain was isolated from a Mycobacterium “Jucho”.
This SM-dependent strain grew in Sauton medium which after isolation contained more than 8μg/ml of streptomycin.
2) SM-dependent mutants gave rise to nondependent cells at the rate of about 3×10^-7. About 98 to 99% of the nondependent cells was phenotypically sensitive and only 1-2 & of them were found to be phenotypically resistant. The phenotypical reverse mutation was suggested to be due to the suppressor mutation.
3) SM-dependent mutants showed R-type colony morphology, slow growth rate on Sauton agar medium and kanamycin resistance. Among these changes accompanied by SM-dependent mutation, the R-type colony morphology and the slow growth rate were lost by the reverse mutation to sentivenss.
However, increased kanamycin resistance remained unchanged even after reverse mutation. It seems that these four phenotypes; the SM-dependence, the R-type colony, the slow growth rate and the kanamycin resistance are controlled by a single gene. On the contrary, kanamycin resistance does not accompany SM-dependence.
4) SM-dependent mutants do not show cord formation in the absence of streptomycin. This finding suggests that the mutants undergo a change of cell surface in the absence of streptomycin.

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For more than five years on 147 cases of far advanced pulmonary tuberculosis classified by the National Tuberculosis Association the author followed up their electrocardiographic right ventricular strain pattern and examined the way myocardiac anoxia or reversible effect its occurence.
Results
In our observation higher grade right ventricular strain pattern are only seen in younger generation, on the contrary, in older age the sign of myocardial injury was observed.

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At the National Sanatoria in our Joint Study Unit a follow up study was made on 623 cases of spinal caries which had been treated surgically up to March 1959.
Method
Using punch cards especially prepared by us, comparisons were made available between two groups; a groups in which spinal fusion was done and a group in which local debridement was done.
Results
1) In the 2nd-3rd decade of their lives most cases were surgically treated, between 3rd-4th decade of life, local debridement was performed predominantly rather than spinal fusion.
2) Patients who had lesions with wide area were most frequently treated with either spinal fusion alone or spinal fusion with local debridement.
But the cases which were treated with only local debridement, generally were patients who had involement of small number of vertebral bodies.
3) Before the operation the patients were generally in the stage of appeasement, and 48.4% of the 623 cases were surgically treated at their maximum stage of the diseases by means of local debridement.
4) Roentgenological survey revealed that 6 months postoperatively those who had local debridement 80.6% had improvement while those who were treated with spinal fusion 45.8% improved.
In the follow up examination after one year 93% out of 623 cases who were treated with the spinal fusion and 91% of the cases with the local debridement revealed that improvement respectively. This indicated that regarding the improvement, minimal difference among the two methods were noted. However, the result with the method of the debridement, was not as good.
5) For Pott's paralysis, both cost-transversectomy and laminectomy were most frequently performed: 39.5% for all the cases. While rachotomy 6.7%, spinal fusion 4%; and local debridement with spinal fusion 3.7%.
6) Each type of chemotherapy was applied for all cases. In 60-70% of the cases the Period of chemotherapy was about 6 months. And in 41.8% of the cases with local debridement chemotherapy was required for over one year. Duration of chemotherapy recently has been gradually prolonged.
7) The variety of the surgical treatments by which the patients within 2 years could return to the place of their business is as the following:
spinal fusion 39%
local debridement 33%
spinal fusion with local debridement 60.9%

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We investigated 56 resected specimens of open negative cavities and we found that eight cases of them showed pathologically open healed cavities. There are some essential conditions with which we are able to expect the healing. These are enumerated as the following:
1) Patient's age less than forty.
2) Regarding the term between the onset and the admission, it was revealed that the shorter the better. Those whoes onsets were more than one year ago showed markedly decreased healing rate.
3) As to chemotherapy, those who have had no chemotherapy prior to the admission showed highest rate of healing, Accordingly those who have had the therapy more than one year showed a notably lower rate of recovery.
4) There was no influence on the rate of healing on those whoes sputum, at the time of admission was negative or positive for tubercule bacilli.
5) Roentgenographic findings were necessitated in the scope such as:
(a) B or C types, (b) 1 to 2 range of spread, (c) Ka, Kb or Kc cavities, (d) cavity largeness 1) and 2) (Gakken classification). But largeness 3) was sometime also acceptable when the cavity was Ka.
6) Roentgenologically those who had remarkably good healing process showed higher probability of healing than those who had only a slight improvement.
7) For the surgical operation the desirable factors ars (a) B and C types, (b) 1) to 2) range of spread, (c) Ka, Kb and Kc cavities, (d) cavity largeness 1) to 2) And as described above, largeness 3) was sometimes acceptable if the cavity was Ka. The width of the cavity wall should be less than 5mm.
8) The average sputum negative period was more than 6 months, the longer the period higher the healing rate.
9) It is indespensable that the chemotherapy after admission of the patients be given for a year or so, however, there was no proof that the rate of healing would rise higher depending upon the long use of the therapy.
But it was important to administer INH for more than 6 months instead of applying above said therapy.

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