Previously we reported that 6 of 13 cases of cold abscess of the chest wall were completely cured with interval injections of Streptomycin (SM) directly in to the lesion after aspirating the pus. We used Streptokinase (SK), Streptodornase (SD) with isonicotinic acid hydrazide (INAH) and SM on 9 cases of this condition. 1.0cc (SK 10.000U. SD 25.000U.) of SK and SD (varidase “Lederle”) disolved in 10cc of normal saline solution was used as an initial dose increasing its amount as time went on.
The method of the application is as follows:
1) After injection of Varidase, aspiration of pus and injection of antituberculous medication was repeated.
2) After a small incision was made over the abscess and the cleaning of the pus and granulation was carried out varidase was injected into the abscess.
3) Instillation of medication was done into the fistula and on the ulcerated area after the spontaneous rupture of the abscess. Following the injection of Varidase into the abscess, a local heat was noted and pain, fever, dizziness and headaches were complained of by patient and sometimes the abscess became enlarged and ruptured spontaneously. Occasionally no signs of easing of the lesion were noted even after the aspiration of pus and repeated use of Varidase. In such cases an operation should be done.
The poor result in the use of Varidase for abscesses and fistulae is caused by: 1) when abscess extend behind the ribs, 2) when caries of the ribs is present. 3) When granulations and Gaseous masses could not be completely irradicated by the use of Varidase. Following the use of Varidase the permeability of the abscess wall may be increased due to the leftover rays of the injected phosphorous isotope (p³².).

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The hemagglutination on the tuberculoss patients published by Middlebrook and Dubos in 1948 has been appraised by various investigators.
It has been confirmed that the results of the test almost parallels with the clinical symptoms of tuberculosis and we were impressed that it is a highly specific test when applied in the active stage of tuberculosis.
However, there are still many aspects yet to be clarified by further studies, namely with regard to the kind of old-tuberculin solution, the pH of physiological saline solution, and the influence of tuberculin injection etc.
In spite of these unsolved questions, the results I have obtained in this test showed a remarkable accuracy viewed along the lines of the clinical symptoms.
By this test it is possible to examine the existence of antibody directly in the streaming blood. This point distinguishes itself from the other serological tests, and some new developments may be expected to come out of this test.

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Glycosiamine in urine of healthy men and tuberculous patients was determined under various conditions as during methionine treatment, during streptomycin therapy and during gelatin administration. The results of the study were as follows:
(1) In the case of healthy men, the excretion of glycosiamine is affected by the diet, especially by its albumen content.
(2) In the case of tuberculous patient the amount of glycosiamine excretion was found less as compared with healthy men. The severer the disease process the less the amount excreted.
(3) The glycosiamine excretion was found less during the methionine ingestion than that of without treatments as compared with the period in which no snch treatment was given.
(4) The glycosiamine excretion during methionine treatment is remakable in healthy men while it is less marked in tuberculous patient indicating a relationship of glycosiamine excretion and the severity of the disease process. In certain serious cases however, the glycosiamine excretion was increased on the first day after the methionine administration.
(5) A temporary increase of glycosiamine excretion is observed during streptomycin treatment. However, it showed a gradual decrease.
(6) Glycosiamine excretion is greater during streptmycin treatment in the slightly and moderately advanced cases while it is less in the serious cases.
(7) When gelatin is administered the excretion of glycosiamine is increased. While it is rapidly excreted by healthy men, it takes longer time in the tuberculous depending on the severity of the disease process.
(8) When gelatin is administered after methionine ingestion the glycosiamine excretion is found less as compared with no such treatment being given.
(9) Almost the similar result is obtained when gelatin is administered after the streptmycin therapy.
From above results we can conclude that the glycosiamine forming function and the function of converting glycosiamine to creatine are markedly decreased in tuberculous patients. It is probable that there might be a functional deficiency other than simple methionine deficiency since the creatie fo formation in the advanced tuberculous patients is not sogreat as in healthy men evenn when methionine is administered.

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In the study of the cultivation of tubercle bacilli in patient's feces I have found following facts and succeeded in developing a new cultivation method by the use of 0.8% Marzonin (Sodium Ethylmerculithiosalicylate) and 4% Cautic Soda.
1) On cultivation of tubercle baccilli in feces, the most common contaminating organisms are bacilli and cocci of various kinds. Fungi are next in common.
2) The number of various contaminating organisms which grow in the course of cultivation of tubercle bacilli varies according to an individual factor. However, in feces of those who are subject to have soft or diarrheal stools it is observed, when cultured, a large number of colonies of various kinds of bacteria on agar plate, and a tendency of marked growth of contaminating organisms.
3) The growth of the contaminating organisms may be checked by giving the patient a daily dose of sulfanilamide for several days before the cultivation.
4) This new tubercle bacilli cultivation method which I have developed and which I call “Marzonin-alkali method” is superior to any other known methods both in securing the number of tubercle bacilli colony and in inhibiting a contamination.

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A comparison is presented of modified Proskaure & Beck's medium with Oka & Katakura's medium for the detection of Tubercle Bacilli in the patients sputa.
We found 109 positive specimens on Oka & Katakura's medium, 111 positive on modified Proskauer & Beck's medium in 161 patients sputa, and a close agrelment of growth rate was found between the two media on the same positive specimen. The colonies of Tubercle Bacilli is found earlier on modified Prokauer & Beck's medium than on Oka & Katakura's medium.
It is supported that the liquid medium is superior to the solid medium for the purpose of early detection of Tubercle Bacilli, although the liguid medium is more frequently contaminated than the solid medium; two contamination were found on Oka & Katakura's medium and six on modified Proskauer & Beck's medium in 161 specimens.
The liquid medium is practical for diagnostic purpose of relatively uncontaminated materials, such as sputum, and we can obtain a good result in early detection of colonies.

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