Originally E, coli was considered as non-pathogenic. Neverthless in many appendicitis cases E, coli was isolated in almost pure culture.
Therefore it was investigated if there is any inflammatory origin about E. coli, here.
In case E. coli was inoculated to the beneath of serious membrane of caecum in guineapig, no matter the E, coli was heterogenic or homogenic to the guinea-pig acute inflammative reaction was observed, but in case the killed bacteria or kaolin was inoculated no inflammation reaction occurred. When the filtrate of E, coli calture was inoculated intra-
venously beforehand then after 24 hours. Suspension of living E, coli was inoculated to caecum wall, phlegmonous inflammation or allergic changes were observed even in 10^-6mg dosis.
In cases no previous procedure was performed, no inflammatory process was observed in such minor dosis.
The same result was obtained in the experiment followed by Shwartzmann's method. The fact proved that E, coli which was inoculated to intestinal wall may cause severe inflammatory reaction in even small dosis.
Therefore it could be concluded that even E. coli which is usually thought considered to be non-pathogenic would exibit severe inflammatory character by chance in certain condition.

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From a water extract of Limachus flavus the fraction of muchinase and several other polysaccharide-splitting enzymes were separated by means of using the lead acetate or ammonium sulfate fractionation procedure. Being histochemically demonstrated, the fermentative activities of hyaluronidase and chondromucinase in their final fractions were ascertained and assayed quantitatively IN VITRO and that of amylase, cellulase and chon-
drosulfatase in the other intermediate fractions were also estimated.

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To treat the tuberculous cavities of the lung the author used the instillation method with the aqueous solution of Streptomycin, Hydrazid, INHG, etc, via bronchus into cavities, This is a similar remedial principle, which in surgical treatment an injury is finally cured on the basis of scars caused by locally applied medicines.
In the therapy with the above mentioned method we expected not only the condensation or diminishing of cavities but also finally their disappearance.
1. The experiments were performed on 30 cases of cavernous tuberculous patients who were with slight or far-advanced forms.
2. Using Metras cannula the aqueous solution combination of SM and INH or INHG alone were given into cavities. For the early period of the treatment these drugs were administered weekly and then later once every other week.
Results
The instillation method revealed a remarkable improvement on the cavities as well as in the general clical features.
1. 24 cases out of 30 cases (76.6%) had favorable results, particularly 14 cases out of the 24 cases showed magnificent improvement; cavities disappearance roentgenologically as well as markedly improved clinical findings.
2. It is concluded that bronchial instillation with antituberculous drugs have proven improved to be a very effective method for treating tuberculous cavities, since there were no noticeable side-effect or undesirable phenomena during the period of the treatment.

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Experiments were carried out to study the effect of chemotherapy in experimental tuberculosis. Mice were given intravenously a strain H37Rv of human type Mycobaterium tuberculosis, and chemotherapy was started either immediately or 3 weeks after the infection. The size of the dose varied from group to group, and the dosage schedules with animals in each group. The animals were divided into 7 groups following the infection, and 5 mg./kg, of isoniazid, 1 mg./kg, of isoniazid, 5 mg./kg, of isoniazid combined with 200mg./kg, of PAS, 5 mg./kg, of isoniazid combined with 50mg./kg, of PAS, 1 mg./kg, of isoniazid combined with 200mg./kg, of PAS, and 1 mg./kg, of isoniazid combined with 50mg./kg, of PAS were given except to one group, which was given no treatment and which served as the control. In each group, the specified amount of drug or drugs were
orally administered erey day, once or twice a week for a period of 100 consecutive days. The determination of the effect of treatment was based on the results of pathologic examination of the lungs and spleen, as well as cultures from them, which were obtained immediately, 4 weeks and 8 we eks after termination of the treatment. Administration of 5 mg./kg, of isoniazid every day had an effect; the specimens were negative for tubercle bacilli on culture immediately after termination of the treatment, but they became positive after 4 weeks, with an accentuation of the histologic picture. Administration of 5 mg/kg of isoniazid twice a week or of 1 mg./kg, of it every day prevented the growth and multi-
plication of the organisms. Under this regimen, however, the specimens did not become negative for tubercle bacilli on culture, and the disease spread rapidly with time. Administration of 5 mg./kg, of isoniazid once a week or 1 mg./kg, of it twice or less often a week produced little effect. The effect of combined administration PAS was noticed only when the dose of isoniazid was small (1 mg./kg, every day). On the other hand, when its
dase was large, it was so effective as to make it impossible to assess the value of PAS used in combination and consequently to make the combined use of the data meaningless. here were scarcely any organisms developing resistance to the drugs in any group.

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No literature has been found regarding circumstances of tablewares contaminated by tuberculous patients and tubercle bacilli. Breakfast utensils served in wards for the open tuberculous patients of a sanatorium were washed with sterile physiogical sodium chloride
solution, and the washing solution was prepared by Ohyama's method. The deposit was smeared on Oka's and Katakurr's culture media and its bacterial growth was observed for six weeks.
Results
For this purpose the experiments were conducted five times. Each time the degree of positivity of bacterial growth on the culture media varies, however, the growth was more or less noted, Niacin-tests (BrCN-aniline tests) were performed on the cultivated bacteria of the second generation and each test proved to be positive. For the purpose of making
histopathological study of the internal organs, the inoculation was admistered to guinea pig3. And the typical tuberculous symptoms were always noted.
Conclusion
1. Bacilli cultivated in the washing solution of contaminated tablewares were mycobacteria tuberculosis var, hominis.
2. The washing solution of contaminated tablewares served in wards for open tuberculous patients suggests the mingling of bacteria which can be apparently examined by the culture.

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Some synthesis and analysis of jaw prosthetic restorations were carried out by the Joint Research Group from April 1955 to March 1957.
1. Clinical cases: 50
2. Maxilla 80% and Mandibula 20%
3. Causes of Lesions: 64%by tumor, the others by injury and inflammation.
By Yoshida's classification form (Deficient appearance of jaw, IRYO 14, 304. 1960) 33 cases who, at the above said installation had both operations and jaw prosthetic restorations, were studied.
These were classified as follow:
Class Case

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One case of primary jejunal ulcer is reported. A 68 years old man with the complaint of abdominal tumor and pain was admitted 32 days after the first episode of crampy pain. Due to the large defined child head sued tumor with tenderness at the epigastrium, the gastric roentgenological findings and positive laboratorical results of urine sugar and of
glucose tolerance test, the case was suspected by us to be a pancreatic cyst.
At the laparotomy 60 days after admission, in the jejunum approximately 45cm below the ligament of Treitz, a perforated ulcer of 2 × 0.5cm was found which was located between the mesocolon and a mass of intestinal loops.
Although the postoperative course was uneventful, a progressive gangrenous change of the left leg necessitated an operation. Two days after amputation sudden death accured.
The autopsy revealed a generalized arteriosclerosis particularly in kidneys, cerebrum and heart. As Letwin etc. reported, it was considered that our case was one of a jejunal ulcer caused by sclerotic changes of gastric arterioles.

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Some statistical observations were made on 102 patients with general paresis in our hospital from 1944-1959. The results are as follow:
1. Number of hospitalized patients decreased gradually, and the ratio between the all hospitalized patients and the patients with general paresis was 12.8% in 1949, 2.5% in 1959.
2. Age of onset was 26-61, average42.
3. Period from onset to death was 1 month-10 years, average 25.8months.
4. Cause of death; 55.3/prostration, 10.0% convulsions and. 12.5%varlous complications etc.
5. Penicillin treatment had a close correlation with death and exacerbation rate and morbidity. And was greatly influenced by the patients' symptoms and the period of the treatment.

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Cases progressive lepromatous leprosy were admin istered 44' diethoxy diphenyl thiourea (provisionally called as Ethoxy TC), pp'dichloro diphenyl thiourea (Provisionally called as Chlor TC) and 4 butoxy 4' dimethyl amino diphenyl thiourea (Chiba 1906) in order to observe the therapeutic effects and side effects as well as the recurrence rate.
Method
The method of administration was Ethoxy TC and TC and Chlor TC were administered in a daily dose of 0.6-1.5g and Chiba 1906 in a dose of 1.5-3.0g. The treatment with Ethoxy TC was given to 3 patients for a period of 8-18 months and Chiba 1906 to 17 patients for 6 -10 months, The administration of Chlor TC was discontinued by the end of 3 weeks due to the development of side effects.
Results
Both Ethoxy TC and Chiba 1906 gave practically no side effects and immediately after starting treatment, showed considerable therapeutic effect. But recurrences began to appear 6 months after treatment was discontinued. Two of the 3 cases receiving treatment with Ethoxy TC and 7 cases out of the 17 cases receiving Chiba 1906 showed recurrence.
Conclusion
It was concluded, in view of the findings stated above, the above said drugs should be used in combination with some other drugs rather than alone. Chlor TC was found to be inadequate for the treatment due to its side effects of anemia and the disturbance of hepatic functions.

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