There are numerous points yet to be solved in the endocarditis lenta. The present study deals with an attempt to make more clearly the bacteriological diagnosis by the study of antibody production of Str. viridans-group which are considered as the chief causative organism of endocarditis, and its precipitation reaction.
Following are the results of the examination: of 23 cases of endocarditis clinically diag nosed there were lb cases of Str. viridans, 2 cases of pneumo-coccus, 3 cases of staphylococcus albus, one case each of enterococcus, anaerobic streptococcus, and pseudromonas. There was also one case of Str. viridans-group in which β-hemolytic colonies were found mixed with α-hemolytic colonies.
I was able to prove the presence of bacteria in urine in certain stages of the disease process not only in cases of bacteremia but in cases in which there were no bacteria found in the blood. This fact was confirmed by autopsy and precipitation reaction.
The patient serum seems to have a specific power to precipitate acid-heated-antigen of the bacteria obtained from the patient suffering from endocarditis. In certain cases however, precipitation was found present by the antigen of str. viridans which were obtained from the urine of the same patient or from other sources in which no causative organisms were found in the blood although endocarditis was clinically diagnosed.
In my observation there was no tendency of alteration of the precipitation reaction evenn after the application of antibiotic treatment.
The serum of febrile patient who was supposed to have a temporary bacteremia reacted markedly to the antigen obtained from his own blood.

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There are many aspects yet to be clarified bacteriologically and clinically of the role that is played by mutated tubercle bacilli. Following are the results of our experiment in attempting to secure more definitely certain relations on this subject.
1) Non-acid-fast strains with no-pigment and red-pigment producing strain (G₂) in the form of mutation were obtained in the colonies of H-strain of human type tubercle bacilli cultivated in Oka-Katakura's medium to which was added 4% rodealin solution. In Kirchner's medium to which was added growth-inhibitory agents of S. M. and PAS solution a similar development of red and yellow pigment-producing mutated H-strains were observed.
2) When H-strain was cultivated in Kirchner's medium to which was added such growth-inhibitory agents as TB1, PAS, OM, SM. and filtrate of glucose-bouillon culture or its alcoholic extract, mutated strains were found to appear in three to four days time. These pigment producing and non-pigment-producing mutated strains were non-acid-fast and Gram-negative bacilli.
3) The mutated bacilli observed in experiments “2” exhibited acid-fast and Gram-posi-tive phase in early stages of mutation but this is a temporary occurrence. After two or th-ree successive cultures they became Gram-negative and produced red or yellow pigments (TII or TIII) or non pigment (TI), We proved that these mutated strains can be reverted in test tube or through animal experiment to the original H₂-strain.
4) That mutation may be brought about artificially on hyman type tubercle bacilli and that it is possible to have it converted from mutated state to the original strain is not only a matter of bacteriologic interest but it call for a close attention in its relation with clinical medicine.
From this observation it seems to me that patient develops immunization to pathogenic bacteria to a certain extent before the clinical diagnosis is established.
On the basis of above results I consider that the Precipitation may have a supplemental diagnostic value if a careful attention is paid on the selection of antigen. This method is particularly useful in these days when the presence of bacteria in the blood is proved with difficulty since antibiotic treatment is usually applied in the early stages of disease process.

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Histamin hydrochloride treatment in place of dionin for corneal diseases was found beneficial.
A lotion composed of 0.5, 1.0, and 2.0% histamin hydrochloride in the physiological saline solution, or ointment form has effected successfully to the corneal ulcer, infiltration of cornea, phlyctenular keratitis, superficial punctate keratrtis, superficial diffuse keratitis, corneal fleck and other conditions. Corneal opacity or irritating symptoms disappeared after 3 or 4 day applications in some cases, However, it showed no beneficial effect in old corneal flecks.

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It is a wide accepted conception that a main active principle of tuberculin is at least a protein in nature. However, in this respect, a notable finding was reported in the previous paper (I. Kasuya, K. Sato, A. Hagitani: I RYO, Vol. 6, No. 3, pp. 197, 1951. ). That is, the tuberculin polypeptide purified by caolin absorption was put on the origin of the paper strip (filter paper TOYO No. 2, 1.5 ×40 cm) and was run by butanol acetic acid water solvent system (4:1:1), showed a ninhydrin positive spot at Rf=0.112; while by the, autobiography. (paper strip was cut into 13 sections with 3 cm imtervals and numerated in series). Each of them was put into also numerated series of test tubes containing 10ml of normal saline, respectively. After the elution of the absorbed substance by 1 hr. incubation at 37°C, 0.1cc of aliquote was injected iritradermally to the tuberculin positive individuals, the maxima of the biological active curve was found to be dropped in the ninhydrin negative zone, i.e. Rf=0.63.
This data is so far interesting that it reveals the presence of a non-protein active principle in the tuberculin polypeptide.
This experiment was done therefore for the search of the chemical nature of this substance with a result as follows.
(1) A nonprotein active principle of tuberculin, which was noted in the paper chromatographic analysis of tuberculin polypeptide in the previous paper, was now isolated as a crystal, through paper-chromatopile, done as a large scale procedure of the former experiment. This substance was also successfully isolated as a colorless needle, by phosphotungstic acid precipitation, baryta digestion and with final ethreal extraction, from the glycine eluate of tuberculin polypeptide adsorbed to caolin, with its melting point being 234-286°C.
(2) 10γ of this crystal developes blue color with butanol solution of 2-6-dichlorquinon-monochlorimid in veronal buffer of PH 7.8; and it shows also deep blue color with Folin-Denis phenol reagent. This indicates that, this substance may have at least OH nucleus in its chemical structure.
(3) This substance elicits, in concentration 0.1γ/0.1ml, positive skin reaction to the tuberculous patients and normal individuals who are positive to tuberculin.
The search of the chemical structure of this substance is now in progress in the laboratory of tuberculosis of this hospital and the chemical laboratory of St. Paul University, Tokyo. And the result of which will be reported in the suitable chemical journal together with the details of this experiment publicated here.

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The fundamental study on this subject was described in Part 1. in which it was proved that the dried serum solution containing penicillin was suitable for preservation of homogenous bone grafts. In this paper are the results of 50 operation cases of bone grafting with the material preserved in the above noted solution in chronic suppurative osteomyelitis, bone and joint tuberculosis, fractures and bonedefects.
The results are as follows:
1) Successful grafting and cure was obtained in 82% of the cases. The result was remarkably good considering half of these cases being of chronic inflammatory condition which is considered relatively difficult to cure.
2) The dried serum solution which contains penicillin proved to be effecacious because it prevents a contamination of bacteria The vitality of bone graft was maintained for a long period of time (23 weeks in the longest) and the degree of idiosyncrasy of homogenous bone was markedly lowered.
3) Segments of ribs removed by thoracoplasty are easily obtainable and there is no danger of infection by tuberculosis.
4) The bones removed from patients under 30 years of age are most desirable. The most suitable period for grafting is not more than 15 weeks storage. The difference of blood types between donor and recipient is of no consequence.
5) On the basis of X-ray and autopsy findings it is considered that while absorption and replacement will take place at the peripheral parts of the transplanted bones no changes are to be expected in the central part of grafted bones.

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In this report, the authors have investigated the presence of a humoral factor inducing the condition of idiopathic thrombocytopenic purpura and have obtained the following results:
1) The serum and the urine of the thrombocytopenic purpura patient produce an anemia in rabbits experimentally. This anemia-producing factor in the serum is soluble in water, but insoluble in ether and ethanol, and that in the urine is precipitated by methanol.
2) The serum of the patient also shows an action reducing the platelets of rabbits. The platelet-reducing factor is not identical with the anemia-producing factor, as the former is not demonstrated in the urine extract.
Summarizing the first and the second reports, the conclusion is drawn as follows:
1) Three cases of idiopathic thrombocytopenic purpura were reported. Among subjective and objective findings, specific hemorrhagic tendency, Fragility of the capillaries, splenic pancytopenia and relative decrease of mature megakaryocytes in the bone-marrow were pathognomonic.
2) The objective findings accounted for the so-called “hypersplenism”, except for splenomegaly which did not exceed one fingerbreadth below the costal margin in all cases.
3) Splenectomy should have been indicated to two fatal cases, as the conservative therapy had poor results.
4) The theory of “hypersplenism” for the pathogenesis of this condition should be modified from the humoral point of view, since these demonstrated factors are most probably associated with the spleen.
5) The anemia occurring in the later stage, which has been attributed to the loss of the blood, could, in some instances, be primary and splenic in its origin.

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The present report is a histochemical study of carbonic anhydrase performed on urogenical and internal secretory organs etc. The enzyme of this anhydrase could be detected and localize in several tissues and cells thus:
1) In cortex of kidney, carbonic anhydrase was found rich in proximal and distal convoluted tubules, anb also small quantity in genital cells of testis.
2) This enzyme could be detected in the folliclular epithelium of thyroid gland.
3) In optic lens the enzyme was found more abundantly in the central part than in the superficial layers.
4) While there was found moderate amount of the enzyme in splenic pulp it was difficult to differentiate it from that derived from red blood cells.
5) A small amount of the enzyme could be found in muscles.
6) This enzyme was found rich in secretory cells of apocrine sweat gland (Osmidrosis axillae) particularly in its secretory process. In eccrine sweat gland this was detected only in epithelium of intercolated tubules.
7) In the central nervous system doubtful granules could be found.

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That Tropolone is a cycloheptatriene was definitely established by Prof. Tetsuo Nozoe and his associates. In order to find out the biological process, it is important to measure the volume of this compound which is held in the body, and the time required for it to be discharged from the body.
It has already been clarified by many scientists that tropolone easily combines with Fe, Br, Ag and others and thus produce color, We have examined whether some color reactions of these compounds could be utilized for the above noted purposes.
Following are the results of the examinations:
1) The color reaction of the tropolone oxide and Fe cannot be used in its low density.
2) The color reaction which occurs by oxidation of permanganate is not a suitable colorimetric determination, because the relationship between the extinction coefficient and the density does not elicit a straight line form
We have obtained rather satisfactory result in color relation of Fe by a solution of acetic acid as the extinction coefficient and the density comes out almost in straight line relation.

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At the time of the clinical application of I N A H, it is very important to have some chemical experiment regard to its pharmacological effects.
In this report I can make clear the absence of the ability of haemolysis in I N A H, by its biological application, and established the quantitative measurement by mean of the electro-colorimetry with para-dimethyl-amino-benzaldehyd in acetic acidity.

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