The present paper deals with the turnover of phosphorus in transplanted malignant tumors and in organs which showed extensive uptake of phosphorus, and phosphorous fractions, such as acid soluble phosphorus, phospho-lipoid and protein phosphorus, by means of radioactive. P³², and with the changes in the turnover rate of phosphorus through x-ray irradiation. As for transplantable malignant tumors of mice, Takizawa's sugar sarcoma, chinon carcinoma and Ehrlich's ascites tumor were tried. As for the organs, liver, small intestine, spleen and bone marrow were taken for the objects. After injection of various amounts of Na₂HP³²O₄, tracer dose was determined, distribution of P³² by time was recorded and the all test animals were autopsied at 24 hours after the injection. From tumors and each organs acid soluble phosphorus was extracted by means of cooled 7% trichloracetic acid, and phospholipid was extracted by means of alcohol-ether (3:1) and total phosphorus was measured by the homogenized solution in physiological saline. The quantity of protein phosphorus was read in the difference between the total amount of phosphorus and the sum of acid soluble phosphorus and phospholipid. Radioactiviy was measured by Geiger-Müller counter with thin mica end-window, and chemical measurement of phosphorus was conducted by Fiske-Subbarow's method. Total body exposure of single dose of 1, 000γ was given to one group of mice, for the observation of the effects of x -ray irradiation upon the turnover of phosphorus.
The phosphorous amount and P³²-uptake of every phosphorous fraction of transplanted malignant tumors and main organs by amounts of subcutaneous injection are shown in the Tables 1, 2. P³²-uptake of every P-fraction of liver, small intestine and malignant tumor through subcutaneous injection of P³² at the rate of 0.05μc one gramm of body weights and changes of the uptake after irradiation are shown in the Tables 2, 4 and Figures 3, 4, 5. The turnover rate of every P-fraction of the three kinds of malignant tumors and main organs are shown in the Figures 1, 2. Changes of the turnover rate after irradiation are shown in the Figures 6-9. Figures 10 and 11 show the changes in P-amounts and P³²-uptake of chinon carcinoma by time after irradiation. Figure 12 shows the turnover rate of necrotic tissues.
24 hours after the P³² administration, protein phosphorus and phospholipid wer convenient for the detection of irradiation, owing to its large uptake of P³².
Proper amount for tracer dose was found to be 0.05-0.1μc per gramm for the subcutaneous injection. No changes was found with this dose in the ratio of P-fraction in liver.
In the later period of the measurement, uptake of P³² was more proportional to the P-amounts compared to the uptake in the earlier period.
In malignant tumors, small intestine and spleen, where metabolism of protein-P was quite active, remarkable irradiation was seen in protein-P. The more active the metabolism, the lesser turnover was obtained: the highest was in small intestine.
P-metabolism in lipoid fraction was effected by irradiation in similar way.
Through irradiation, metabolism of acid soluble P was not markedly affected.

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The authors tried a new barium opaque, “Barer Relief”, for the x-ray relief of digestive organ, by means of Acacia powder, Mineral oil and Barium, in which Acacia powder works as protective colloid. Our opaque, “Barex Relief” is composed of 20cc of mineral oil, 10g of acacia powder, 30cc of water and 104g of barium. The authors applied the “barex relief” in 450 cases and obtained successful relieves in their x-ray diagnosis. For the x-ray examination of oesophageal mucosal pattern, patients were given one tea-spoonful of “barer-relief”. As soon as the excess passed through oesophagus, generally, the residual layer adhered to the walls so that a sharper and clearer images could be obtained for several minutes. Moreover, our method was proved to be efficient in the early diagnosis of small infiltrating lesions of lower oesophagus, where ordinary barium relief failed to give pathological picture. Three cases of oesophageal varices were detected in our patients with cirrhosis of liver. For the x-ray examination of gastric mucosal pattern, our procedure was to use one tablespoonful of “barer-relieft”, which was distributed over the mucosa of stomach through palpation at various positionings by the patients. This method was proved to be efficient in the visualization of gastric mucosal pattern on a single x-ray film, especially, of the total region of stomach and full defects, ulcerations or various pictures of gastritis. For the x-ray examination of intestinal crassum mucosal pattern, “fbarex-relief” was diluted 3 times with lucid water and the double contrast technique with Fischer's method was conducted. The method was successful in the detection of inflammation, polyp, and ulceration of intestinm crasum.

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It is well known fact that TB bacilli are detected from old latrine ponds of TB sanatoriums for a considerable length of time. However, no reports have ever been preceded regarding the influence exerted upon the farmers when excrements and urine are used as fertilizer in agriculture. Therefore, considering that an attempt of study given in this phase would be worthwhile from the standpoint of preventive medicine and hospital adoministration, the present study was conducted.
The authors obtained the data that the excrements of our sanatorium had been utilized by the local vegetable association (consisted of 27 families and of 162 persons) for the past 12 years, The sprinkling area was localized and the drift of the farmers was scarce in number. During the period from 1942 to 1947 when the supply of fertilizer was extremely short, that is in the “most heavily utilized period”, excrements being in great demand, it resulted in dense sprinkling without taking the trouble of decomposition. Considerable number of younger persons were obliged to help in agriculture due to lack of manpower during the war. The fact is obvious that the period of 8 years was comparatively worse in condition than that of to lay. With the above data in hand and by taking a control group (consisted of 27 families and of 172 persons) in the same area and yet having no relation whatever with the excrements of the sanatorium, a comparison of the two groups was made to observe the relation of presumptive degree of the influence of the excrements.
1. The % of positive tuberculin reaction indicated “association” group-83.3% and “cqflrast” group-81.8% as to the ages of 21-30 who could have been most affected during the “most heavily utilized period”. As in the rest of age classes, there is no significant difference detween the two group.
2. Although the affected percentage of x-ray examination in the 1-40 ages class showed high rate in the “association” group, it revealed an adverse result in the rest of age class, thus it cannot be concluded that there is an influence of the excrements. As to the age between 21-30 where the influence could be mostly considered, the percentages were 45% in “association” group and 29.4% in “contrast” group. Thus, there is no significant difference from the point of view of stochastic.
3. A study was made to find the diference of the positive percentages of tuberculin reaction in those who were exclusively engaged in agriculture. The results were “85.2% in group, 78.2% in “control” group and the affected percentages of x-ray examination were respectively 36.7% and 35.3%. This shows no significant difference between the two groups.
4. Further research was made to see the tuberculin positive reaction of the entering grade school children for the past 12 years when the inflence of B. C. G. was very small. The results were 8.2% in the former and 13% in the latter, thus there is no signifecant difference.
5. Numbers of TB patients were respectively 9 and 10 and in which only 1 each ha l any relation with the presuptive infections source.
It is concluded from the above results that although factor of infection may be anticipated, our data indicated that the usage of excrements of TB patients does not render any influence to the farmers.

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In order to find about the prognosis of tuberculous ex-soldiers, the authors conducted a follow-up study on the far prognosis for the period from 7 to 13 years in 2, 160 TB veterans. On their initial contact and duration of hospitalization, our previous paper was reported. In this paper a statistical observation was made on the symptoms at the time of hospitalization in relation to their prognosis.
1. At the time of hospitalization 50% of the total cases suf fereed from infiltrated pulmonary tuberculosis, out of which 40% of cases with scattered TB regions were fatal and 85% cases with mixed pulmonary phthisis were fatal.
2. Among the above cases, 35-55% became ill in Japan proper.
3. Out of the 2, 160 cases, 1, 649 had pathological findings in lungs, of which 37% had cavities. Only 11% of them survived.
4. Rib-anomalies were found in 1.88%, and they were most frequent in the right III. 5 cases gad bob-fragments in their lungs. One case had his heart in the right.
5. At the time of hospitalization, 56, 5% were found to be sputum-positive for TB bacilli; 40.80, negative. 61.5% of the positive cases died, while 22.1% of the negative cases were fatal.
6. At the time of hospitalization, 61.5% cases had fever of 37.5°C or more and 2/3 of them died Meanwhile, 15.5% of the rest cases were fatal.
7. The cases whose erythrocyte-sedimentation rates were high at the time of hospitalization, had worse prognosis; i. e., 82.9% of the “high” cases died, and 1.9% returned to their works. Meanwhile, 11.2% of the normal sedimentation cases were fatal, and 25.7% returned to work. 8. 13.4% of the total cases had haemoptoe during their hospitalization and 75.4% of them were fatal. 13 cases died from lung bloodings.
9. As for clinical status at the time of discharge from sanatorium, 43% were arrested type; 27% were active type and 29% were fatal. 44% of the active type cases died after discharge.

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Sensitivity tests were conducted upon one hundred and fifty-three strains of staphylococci, which were isolated from the clinical materials at the 1st Tokyo National Hospital during the period from January 1954 to September 1954, against seven antibiotics. The following results were obtained:
1. Many strains were resistant against Penicillin and Streptomycin. Especially, the number of Penicillin-resistant strains of coagulase-positive strains reached to 84.2% of the total.
2. Comparatively few strains were resistant against Chloramphenicol.
3. The resistant strains against Oxytetracycline and Chlortetracycline were about one-third of the total strains. Most of the coagulase-positive strains which were isolated from feces were more than 100γ/cc resuscitable. Cross-resistancy between Oxytetracycline and Chlortetracycline was observed.
4. There were few resistant strains against Erythromycin and Carbomycin. It is assumed that the number of resistant strains will increase in the further investigation.
5. There was considerable number of resistant strains against two or three antibiotics simultaneously. It is also assumed that the number of such strains will increase in the course of further tests.

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