1) During the fluid therapy of surgical patients, the author found that the serum potassium level of a patient can be largely influenced by the conditions and techniques of separating-cell elements: (dry or wet syringe, test tube silicon coated or not, use of heparin, environmental temperature and length of time after extraction of whole blood, centrifugation etc.)
In order to minimize these errors, an absolute method was established: (1) prepare isotonic and iso-electrolytic heparin solution (Table 2) aseptically, (2) moisten the syringe with this solution, (3) extract the venous blood without any oppression on vein, (4) pour the whole blood into a test tube not silicon-coated, (5) centrifuge for 6-7 min. at 2000, r. p. m, within an hour after the extraction of blood from the patient, (6) remove the plasma into an other tube at once.
2) To verify the clinical usefulness and accuracy of this method, a series of experiments were performed on normal subjects and patients (Fig. 1-6).
3) Normal human plasma potassium level was reexamined by this method. The range, that has been considered to be wider than that of plasma sodium level, was proved to be as narrow as that of sodium.
4) Sixty four surgical patients were administered 20 mEq or more of potassium intravenously in an hour. The plasma potassium level was checked again just after the administration or on the following morning, and it showed no harmful elevation in any cases, even though there were some paradoxical falls of its level.

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The experiment was made on the division of the sermoprotein by changing in many ways the density of the veronal buffer being used with the paperelectrochromatography. It was thereby found that the division of the figure of sermoprotein was in the best condition when the following buffers were employed:
1) Buffer A (pH 8.6) Veronal-Na 7.73g (Sodium-diethyl barbiturate) Veronal 1.38g (Diethyl barbituric acid) add Aq. dest. 1.000cc
2) Buffer B (pH8.5) Veronal-Na 11.76g (Sodium-diethyl barbiturate) Sodium Acetate 6.24g N/10 Acetic acid 107.7cc add Aq. dest, 2, 000cc

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On the isoniazid resistant bacilli isolated from the pulmonary tuberculous patients, the catalase activity and virulence were examined.
The virulence was determined by macroscopic changes of various organs as were the viable units contained in the spleen of the guinea pigs at autopsy 8 weeks after subcutaneous injection of the bacilli.
In the 13 resistant strains used in this experiment, 100γ/ml isoniazid resistant strains showed negative catalase reaction and attenuated virulence, on the other hand the strains lower than 10γ/ml resistant had not always clear relation among them.
The guinea pigs were treated with INH given orally 4mg per day for 10 to 21 weeks after the subcutaneous inoculation of 0.1mg of Wy jml isoniazid resistant and catalase negative strains.
This treatment was more effective compared with untreated groups, but the virulence of bacilli, which were isolated immediately after the treatment for 21 weeks and 11 weeks after the treatment for 10 weeks, did not show any change.

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As described in my previous report, out of 57 patients, resistance was noticed 88.6% to SM, 66.0% to PAS and 52.8% to INH. These patients were treated with PZA 2.Og and INH 0.4g daily for six months.
1) Sputum smear positive into negative-Within one week of the above mentioned therapy we found some sputum smears showed tubercle bacilli positive into negative. This tendency was noticed as maximum after two months therapy. However, we had some cases in which their smear test became positive again, especially after one or two month's therapy. This phenomenon was noticed among 50% of INH resistant strains.
These facts indicate that the efficiency of PZA on tubercle bacilli is quick and consequently it does not last long, therefore no one can expect this to be efficient for a long time. This tendency was particularly remarkable in the INH-resistant strains.
2) Rentogenographic improvement-Most patients had disseminated infiltrates.
(1) INH sensitive group-moderate and marked improvement was found in 4.4% after 3 month's therapy and in 40.0% after 6 month's therapy.
(2) INH resistant group-after 3-6 month's therapy 25 of them showed improvement.
As for cavities no case had cavities that disappeared by 6 month's therapy. However.
(1) INH sensitive group-cavities became smaller in 12% by 3 month's therapy and 24% after 6 month's therapy.
(2) INH-resistant group-only 10.7% showed improvement.
3) Toxicity-Hepatitis was rare among them. Joint pains were 89.5%, hemoptoe was 4.3%, hemosputum 10.6%.
Summarily, PZA-INH therapy for 6 months brings improvement in sputum bacillus test, rentogenographic findings, and general condition of the patients. This indicates we have to observe the patients for a long period of time.

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For the period of three to eight months observations were made on 47 pulmonary tuberculous patients with whom Spa-bathing at the temperature between 39°C and 40°C was used. According to the standard of N. T. A., 29 cases were classified as of slight illness and the rest 18 cases belonged to the illness of the middle stage.
During that period body weight, body temperature, bacillus examination in sputum, BSR, chest x-ray photo and subjective symptoms such as cough, sputum, change of appetite were examined respectively.
Results are as follow:
1) Body weight-increase 63.8% and decrease 27.6%.
2) Body temperature-no change.
3) BSR-delay 57%, no change 10.6% and increased 31.6%.
4) Bacillus examination in sputum-disappear 70%, decrease 20% and increase 10%.
5) Chest x-ray-better 55.3%, no change 42.6% and worse 2.1%.
Judging from these results we are able to conclude that if the patients are put under the physician's adequate guidance, they will be no impediments for the tuberculous who are given Spa-bathing therapy.

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Recently in the United States several cases are reported on the simulation of pulmonary tuberculosis by atypical acid-fast bacilli and numerous studies have been made on the characteristics of such strains.
During our daily business, we believe it doubtful whether it is adequate to name these non-tuberculous bacilli same as atypical acid-fast bacilli or yellow bacilli. Due to the fact that in nature non-pathogenic acid-fast bacilli are widely distributed and also these bacilli are often found in our laboratory from the various kinds of speciments, we believe we should differentiate it with utmost care.
In purpose of studying the chromogenic acid-fast bacilli on Qgawa's egg culture medium we have investigated 17 strains isolated since 1955.
1) Isolation frequency; The frequency of happening isolation from the specimens were as follow:
(1) Sputum-12 out of 2, 142 cases (0.56%).
(2) Urine-3 out of 158 cases (1.9%).
(3) Gastric lavage and others- (0) out of 196 cases.
2) Character of colonies and morphology of cells: The colonies of almost all the strains were yellow or orange in color and smooth, soft and creamy in form. Their viscosity were found to be strong, however, they were easily homogenized into smooth cell suspensions. Morphologically, most of them resembled the human typed tubercle bacilli and some did not. In general, they were polymorphylic.
3) Preis test: Almost all the strains had decolouration in less than 2 minute's boiling.
4) Anti-alkaline test: Viability was not lost after treatment with 4% NaOH solution for one hour.
5) Influence of light on chromogenesis: Pigmented colonies grew either in the dark or in the light. In other words, they were all scotochromogens.
6) Sensitivity test for anti-tuberculous substances: All of the strains displayed resistance toward SM, PAS and INH with some difference in degree.
7) Neutral red test: All were found to be negative with neutral red test by Hughes method.

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In pulmonary tuberculosis examinations were made on the relation between PBI (protein bound iodine) and functions of the hypophysis and adrenocortical glands. Bassed upon confidential limit of PBI rate among the healthy human beings, as previously described, PBIr ate has been classified into three groups; a) higher-more than 5.85γ/dl b) ordinary 5.85-55.22γ/dl and c) lower-less than 5.22γ/dl.
1) Average PBI rate in pulmonary tuberculosis is 5.9γ/dl (3.2-8.3γ/dl) and compare with the healthy human beings, this rate varies widely. There were more higher groups and less ordinary groups.
2) On the relation between the condition of diseases (NTA classification) and PBI rate, there was no significant difference among higher and ordinary groups whileTcases which belonged to the lower groups were found among the cases of moderately advanced or of far advanced cases.
On the other hand, according to the pulmonary tuberculosis classification by Gakken, there was no significant difference in the higher and the ordinary groups while in the cases of the lower group it was found more among B and F types rather than A type. And also in the same group it was found more among B and F types rather than C type.
3) No specific constant relation between doses of paraaminosalicylacid andPBI rate was recognized.
4) Cases with the normal adrenocortical functions were seen more in the ordinary group and there were seen less in the higher or lower groups.
5) Normal shaped sella turcica was seen in the ordinary group and abnormally formed sella turcica was seen in the higher or lower group.

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On 20 patients of appendicitis, we have observed the changes of the N. P. N. - content in the serum during pre-- and post--operative periods of appendectomy.
In every patient, N. P. N. was measured by mikro-Kjeldahl method 4 times, that is, at first just before the operation, 24 hours and 72 hours after the operation, and at last 6 days after the operation.
The results obtained were as follows:
1) The rise in the N. P. N. -content of the serum was seen in all patients 24 hours after the operation and it returned to the pre-operation value 6 days after the operation in all patients.
2) Between the patients who were administered chlorpromazin and not given it, there was no marked difference in the changes of N. P. N. - content.
3) In the group of acute type of appendicitis, the N. P. N, content was higher already before the operation, and the rise after the operation was also higher than the group of chronic type.

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