It is said that the marked advances was made in diagnosis of SLE since LE cells phenomenon was discovered by Hargraves in 1948. LE cells phenomenon is a important factor in making diagnosis of SLE.
There are many diagnostic criterias in SLE by famous authors and Committees, for instances-by Stevens, Dubois, MRC and ARA. Among these, there are some differences, that is, like Dubois' criteria some signs and items have larger weight and like criteria of ARA all signs and items have same weight for diagnosis of SLE.
This paper was described in the attempt to discuss whether LE factor would have same or larger weight in the all items of criteia. 45 cases of positive LE test (Hyland Co. ) and 105 cases of negative LE test were picked up at random in collagen diseases, liver diseases, renal diseases, blood diseases, allergic and so on.
Then the results were obtained as follows-
1) LE test was positive in about 80% of cases of definite SLE and in about 70% of cases in definite and suspected. In the other diseases there was no cases that LE test was positive over 50%, and LE test was positive in 20-30% in liver diseases, tbc., PSS, and so on.
2) LE cells was positive in about half cases of SLE and in rare cases of the other diseases. In SLE about 60% of cases of positive LE test revealed positive LB cells phenomenon and in others only one cases of positive LE that revealed positive LE cells phenomenon. In SLE one of negative LE test was positive LB cells and in others all of negative LE test was not negative LE cells. Then positive relationship between LE test and LE cell phenomenon was recognized and LE test was more sensitive and less specific than LB cell phenomenon for making the diagnosis of SLE.
3) In the cases of positive LE test, higher titers of B. S. R., rather leucopenia, more positive RA test and higher titer of γ-Glob, were recognized. But the factor made positive LB test was not discussed.
4) In making the diagnosis of SLE typical skin rash and LB factor etc, may be supposed to be rather more important

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Sterile women with suspected ovarian dysfunction were tentatively classified on the basis of the BBT and the periodicity of menstruation, and a systemic treatment was applied for the induction of ovulation in each group.
This paper presents some results which have been obtained in our clinic from Dec. 1970 to Nov. 1972.
Reliable BBT were obtained with 622 cases, of which 65.5% belonged to regular diphase-eumenorrhea, and 18.0% to low monophase: the latter is seldom associated with spontaneous ovulation.
The Kaufmann therapy was performed in 29 cases (one course for each case), and the rebound phenomenon was noted in 9 cases, of which 4 became pregnant. A total of 387 cycles of clomiphene were administered to 109 cases, and 34 became pregnant.
The HMG-HCG therapy was applied to 24 cases, in which the clomiphene therapy had previously been ineffective. Ovulation were noted in 12 cases, 3 became pregnant during the administration, and additional 4 pregnancies were found in half a year. Of the whole pregnancies found within the two year observation period, about 40% were considered to be the effective cases for our ovulation-induction therapy

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Clinical symptoms in hypercalcemia by carcinoma of the breastare apt to escape from attention, because they resemble general symptoms of patients with carcinoma in the terminal stage. Even if the diagnosis is appropriately made, the prognosis is poor, resulting in death with 3 to 6 months from the first hypercalcemic crisis. This paper reports the clinical aspects and the therapeutic experience of hypercalcemia by thyrocalcitonin.
The initial symptomatology of hypercalcemia includes thirst and polyuria in renal function;nausea, vomiting and anorexia in gastrointestinal tract; and apathy, headache, convulsion and coma in nervous system.
Objectively, tachycardia, shortening of Q-aTc time in electrocardiogram and elevation of serum calcium and alkaline phosphatase are noted. When bone metastasis of breast carcinoma is diagnosed or suspected, it is important to check whether hypercalcemia is present or not and not to regard these symptoms as general complaints observed in patients with advanced carcinoma. In carcinoma of the breast, one of the causes of hypercalcemia is osteolytic bone metastasis, but the others are parathyroid hormone-like substances derived from tumors and osteolytic sterol, we think.
And also the fact must be taken that hypercalcemia is derived from sex hormones which are utilized to bone metastasis of breast carcinoma. As the treatment of hypercalcemia, massive transfusion and steroids have been used in order to decrease blood calcium level and to excrete calcium from urinary tract. Nevertheless, these treatments were temporary, and the patients died of renal and respiratory insufficiencies due to calcification of kidney and lung. To apply thyro-calcitonin, which make the level of blood calcium lower, to the patients with hypercalcemia, we tried to use porcine lyophilized calcitonin (Armour Pharmaceutical Co. ) to two patients with bone metastasis by breast carcinoma. As a result, one is prominent in effect and another is temporary, and serum calcium of both cases was controlled during relatively long term. These cases are now under followup

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Cholinesterase Isozyme in serum was measured by the plate polyacrylamide method. The band II was removed with disease, and went down in liver cancer and brain tumor, and specially in lung cancer

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There were 113 cases of surgical treatment on the non-tuberculous pulmonary disease from 1968 to 1971 in the Chubu-Byoin National Sanatorium.
We described on the results of surgery of 31 cases of the pulmonary cancer, 17 cases of the bronchiectasis, 16 cases of the pulmonary suppuration, 17 cases of the spontaneous pneumothorax and 5 cases of the giant air cysts.
We must be careful in the surgical treatment on the next points.
(1) the mass surveys for early diagnosis and early therapy of lung cancers.
(2) the active operation for the bronchiectasis and the pulmonary suppuration of surgical adaptation.
(3) the surgical criteria for the spontaneous pneumothorax and giant air cysts

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Previously, Tsukamura, Mizuno and Tsukamura (Medicine and Biology, 73: 64-66, 1966) reported that the measurement of the radioactivity of whole cells after incubation with ³⁵S-methionine was useful for differentiation between M, intracellulare and M. avium, and M. scrofulaceum.
The present study reports that thin-layer chromatography of ethanol-soluble fraction after incubation with ³⁵S-methionine is useful for differentiation among mycobacterial species.
The test organism was cultivated on Ogawa egg medium at 37°C for 4 days (rapidly growing mycobacteria) or 14 days (slowly growing mycobacteria). The organism grown was washed three times in saline and weighed aseptically. 100mg, moist weight of the organism were suspended in 2.0ml, of 0.067M phosphate buffer solution (pH 7.1) containing 2.5μc./ml. ³⁵S-methionine and incubated at 37°C for 24 hours. The ³⁵S-L-methionine used was a product of the Radiochemical Centre, Amersham, Buckinghamshire, England (specific activity, 135 mCi./mM).
After incubation, the organism was washed three times with 2.0ml, phosphate buffer and extracted twice with 2.0ml, of 10% trichloroacetic acid (TCA) and then extracted twice with 2.0ml, of ethanol. The residue was dissolved in 2.0ml, of 1% NaOH solution by heating at 100°C for 5 minutes. The residue solution was named protein fraction. 0.2ml, sample of the fractions was placed into a stainless plancht and dried by heating. The radioactivity of the sample was measured by a gas flow counter.
After measurement, the ethanol-soluble fraction was concentrated under reduced pressure (2mm. Hg) and the concentrate was subjected to thin-layer chromatography. The thin-layer chromatography was carried out according to the procedure described by Marks, Jenkins and Schaefer (Tubercle, 52: 219-225, 1971), using the Silica Gel H according to tahl (E. Merck, Darmstadt, Germany). The solvent used was n-propanol+n-butanol+water+ammonia (57+20+20+3 by volume ratio). Developed chromatogram by unidimensional run was dried and radioactive spots in the chromatogram were recorded by an automatic scanning apparatus (Nihon-Musen Co., Tokyo).
The results of the measurement of the fractions showed a considerable variation, but the estimation seemed to be useful for differentiation between a few species, for example differentiation of M. avium and M. intracellulare from M. scrofulaceum (Table 1).
Thin-layer chromatography of the ethanol-soluble fraction seemed to be useful for differentiation among mycobacterial species. The mycobacterial species showed a pattern of distribution of radioactive spots almost specific for the species (Tables 3 and 4). A few species showed two patterns, for example, M. intracellulare, M. borstelense, and M. smegmatis

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It is often observed that various pulmonary diseases, particularly pulmonary emphysema, bronchial asthma, and chronic bronchitis cause accumulation of CO₂ in body due to low alveolar gas exchange. Measurement of alveolar pCO₂ is necessary to make a confirmed diagnosis of hypercapnia.
We evaluated a clinical utility of a “screening CO₂ analyzer”developed by Suematsu with a rendered complexity compared with other apparatus.
(Experimental)
A patient was ordered to make a forced expiration following resting state respiration. Breathed air was collected on the moment when the patient finished the forced expiration. Gas volume was measured by a mercury manometer before and after reaction with a CO₂ absorber (saturated KOH) for 1 minute.
PACO₂, the alveolar partial pressure measured by this method, was compared with PaCO₂, arterial CO₂ content, in 80 cases including healthy volunteers and patients of various lung distresses.
(Result)
Correlation of PACO₂ and PaCO₂ was expressed as n=80, r=0.857, and y=0.84x+3.41. The r value of 0.83 was obtained when we started learning this procedure, but after experienced well, the value rose up to 0.89. This may indicate a need of skillfulness.
We assessed a normal value of PACO₂ using values of subjects who gave PaCO₂ of 35-45mmHg. Normal, 34-43mmHg; suspicious of hypercapnia, 44-48mmHg; Abnormal, 49mmHg or more.
(Conclusion)
PACO₂ value is closely correlated with PaCO₂, thus seems to be substitutable for PaCO₂ measurement to diagnose hypercapnia, particularly when a simpleness and quickness is required on a bed side, or to screen out abnormality from a number of cases in a short time.
The most essential point in the technique is to collect air at the finish of forced expiration.

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On serums of 951 tbc, patients, 157 non-tbc, patients and 150 healthy men, Caolinaggultination test and complement fixation test were examined with dried antigen obtained from tbc, bacilli. The results were as follows:
1. The positive rate of the two tests depended upon the exsistence of tbc. bacilli in sputum and the activity of the disease.
2. Of the tbc, patients, the positive rate was 44.4% by aggultination test and 52.8% by complement fixation test.
3. Non-specific reaction was found more in the aggultination test than the other.

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Two cases of neonatal gastric perforation were reported in 4 and 5 days old boys. The birth weights of the cases were 2, 660g and 2, 700g respectively. They didn't have vomiting. Scout film examinations of their abdomens in upright position revealed a large amount of free air. The first case died seven hours after operation and the second case died thirteen hours after surgery.

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Both of two fatal cases (one-egg twin) of the progressive muscular dystrophy (Duchenne type) showed quite similar clinical course and electrocardiographic findings, and resulted in heart failure death.
Until about 1 year before death, their electrocardiograms were of characteristically“right ventricle-dominant”state, after that, they coverted to be characterized as those of failured heart.
Autopsy findings of the two were characteristic of congestive sign in whole bodies, with the myocardial involvement revealed on similar location in each heart.
After all, those twin cases were respected on the most representative clinical picture and cardiac involvement in progressive muscular dystrophy.

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(1) The number of young patients in the solitary island were few.
(2) Patients who were in need of otorhinolaryngeal treatment were found in 79 out of 279 cases. Patients who were in need of otorhinolaryngeal operation were found in 30 out of 279 cases.
(3) Indistinct perceptive deafness, chronic rhinitis, chronic sinusitis, chronic tonsillitis with stomatitis, chronic tonsillitis with tooth caries, and neurosis of the larynx, etc. were found frequently on the patients who lived in the solitary island.
(4) The need of treatment of otorhinolaryngeal disease in the solitary island is keenly felt. Sanitary problem of those who live in the solitary island must be considered.

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