In order to find some reliable and easy serological parallel diagnostic measures for dysentery's bacterial dell agglutination, sensitized erythrocytes agglutination, sensitized erythrocytes hemolysis and their inhibition tests were simultaneously carried out. In cases of immune rabbits' serum, the specy and subtype of infected bacillus were able to be determined by the all tests. However, in the serum of clinical cases, it was unable. It should be considered that the recovery from dysentery is instant, so that the immunity to dysentery is not accomplished and the amount of antibody is little.

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By means of paperchromatography, the author conducted qualitative measurements of free amino-acid in tuberculous pulmonary cavities and encapsulated caseous foci. The results were as follows:
1. From the contents of cavities and encapsulated caseous foci, cystine, asparatic acid, glycine, alanine, arginine, proline, valine and leucine were detected.
2. In the average, the cavities contained per focus 5 kinds of free amino-acid and per encapsulated caseous foci 3 kinds of the same.
3. In case of cavities, cystine showed the highest rate of detection and followed by asparatic acid, alanine, proline, glycine, leucine, valine and arginine in rank. In case of encapsulated caseous foci, the rank was graded as follows: Cystine, asparatic acid, proline, alanine, glycine, arginine, valine and leucine.
4. In case of cavities, there were no considerable differences in the number of free aminoacid by size of the cavities. While in case of encapsulated caseous foci, number of the kinds of free amino-acid increased in proportion to the size of the lesion.
5. As for relation between free amino-acid and bacilli, in multibacilli such as type D and type Z, many kind of free amino-acid were revealed and few in the few bacilli group such as type W. In encapsulated caseous foci, positive bacilli group had more number of free amino-acid than negative bacilli group.
6. In softening encapsulated caseous foci, number of free amino-acid was more than in nonsoftening ones.
7. Between free amino-acid found from cavities and the dose of antituberculous drugs, no remarkable relation was revealed. It was recognized that newer foci had more free aminoacid than older ones.

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The authors divided far advanced pulmonary tuberculosis cases into four groups and gave them treatments as follows:
Group A: 0.3g of INH per day continuously for three months;
Group B: 0.3g of INH and 3g of Sulfathiazole every day continuously for three months;
Group C: 0.3g of INH and 3g of Sulfisoxazol per day continuously for six months;
Group D: 1g of INHG-Na and 3g of Sulfisoxazole per day continuously for six months.
The mutual comparisons of effects of the treatments were as follows Group A: In comparison with the previous data (Ryoken, 1956) where only INH for 3 months was the treatment, Group A cases showed a little not good in boody weight and appetite, however, a little better in findings of X-ray examinations and in view of resistance. As most of the present cases were far advanced TB, they had been treated with much INH before our treatments.
Group B: These cases generally showed not good results, especially, in appetite, body weight and TB bacilli tests in sputum and side reactions.
Group C: Generally, these cases showed results that were equal to Group A. They were good slightly in body weight and appetite and no good in blood sedimentation rate and TB bacilli in sputum. They were superior in view of resistance to drugs.
Group D: Better in body weight, appetite, clinical symptoms and control of resistance, particulary in view of side reactions. The authors conclude that the joint treatment with INH and Sulfisoxazole, especially with INHG-Na is very effective for the treatment of serious pulmonary tuberculosis.

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The author previously reported that the cutaneous touch stimuli mainly cause temporary augmentation, while the cutaneous pressure stimuli will mainly cause inhibition upon the pneumogram and EMG in rabbits, however, the alternative action of the respiratory center, alternation of respiratory movement, was not mentioned at the last report. In the present paper the author is to report the results of the experimental data quantitatively, and to state the following evidences as to the alternative action of the respiratory center:
1. Cutaneous touch stimuli caused temporary augmentation upon the alternative action of the respiratory center.
2. Cutaneous pressure stimuli caused inhibition upon the alternative action of the respiratory center.

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As it was reported previously, in TB cases the decrease of Na-content in the rib bone presumably did not reveal the entire picture of electrolyte metabolism of osseous system. However, it is interesting to follow the postoperative evolution of serum concentration as well as urinary excreation of Na. In the present study, further observations were made at postoperative period in order to determine relationship between Na in serum and in urine excreation, and electrolyte contents of bones and muscles. The results and conclusion were summarized as follows:
1. On the, second postoperative day, serum sodium and chloride concentrations markedly decreased and the urinary postassium excretion increased although serum potassium concentration was variable.
2. These postoperative electrolyte alterations in serum and urine were incompletely recovered even on the 7th postoperative day.
3. It was observed that there was no close relationship between the preoperative value of sodium contents in rib and muscle, and the postoperative serum concentration and urinary excretion of electrolytes.

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The influence of single or combined continuous administration of SM, PAS, TB₁, and or INH on the migration velocity of neutrophile leucocytes of pulmonary tuberculosis was examined and the following results were obtained.
1. Some cases showed decreased migration velocity before the administration. Most cases showed increased migration velocity 10 days after the start of the therapy. It gradually increased and recovered the former value by 1 month, exclusive of the cases administered with PAS alone, which presented lower value than that befor the administration.
2. The increase was marked in those cases that had shown abnormally low migration velocity before the administration. Slight variation was observed in the cases whose values had been nearly normal. Only a few cases showed supernormal value after the treatment and the majority of the cases showed normal level continuously.
3. Relation between clinical findings and the migration velocity differed from case to case. By chemotherapeutics used or methods of administration adopted, no appreciable difference was observed. However, cloose parallelism was found between clinical findings of each case and migration velocity. In the cases where chemotherapy was efficacious, increase of the velocity was also marked.
These results may confirm that migration velocity of leucocytes will increase when antituberculous agents are administered in pulmonary tuberculosis. It is not clear whether this phenomenon is due to direct effect of these agents on the function of leucocytes or to the secondary change evoked by the inhibited growth of TB bacilli.

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Since January, 1956, the authors conducted clinical experiments on antituberculous agents; physiological saline solution of streptomycin isonicotinyl hydrazone sulfate, 40mg per ml or sodium glucuronate isonicotinyl hydrazone, 300mg per ml, in the treatment of surgically contra-indicated cases with cavities. As for the methods of administration, together with routine administration, intracavitary injection through the selected bronchi was conducted by means of Métras' catheter from 3ml to 5ml of size, in proportion to the sizes of cavities. The treatment was conducted on once a week on 29 cases for more than 30 weeks. Results showed that 4 cases turned negative of TB bacilli both in smear and culture tests, and another 4 cases changed to negative in smear examination. Effect upon cavities was also prominent: 5 cases showed reducing cavities markedly and 6 cases showed reduction of cavities. Serious side effects were not provided in this treatment. This treatment shall be considered as a good measure to be tried concurrent with routine treatments. It is believed that psychological effects to the patients may be concerned with.

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