TB-I therapy, for a period of 10 weeks, were studied on 27 tuberculous patients at the Second Hokkaido National Sanatorium.
In the cases administered with TB-I; the presence of disturbances of liver function and its occurence in time, was searched through the examination of liver function and reached the conclusion as follows:
1) A marked disturbance of liver function was not found by serial administration of TB-I gradually increased (the maximum dose 100mg); in a couple of cases only a temporary and slight disturbance was observed and in very few cases, a tendency for improvement was found.
In general, in the Hepatosulphalein test, a temporary and slight disturbances occured in several cases and 4 showed improvement.
Also in cases of the serum cobalt reaction and the serum cadmium reaction, a remarkable disturbance of liver function was not found. However, in a few cases, tendency for improvement was found,
In the urine no marked changes of urobilinogen reaction were found.
2) In the second to the sixth week, after administration of TB-I, the disturbances of liver function were found most frequently, then tended gradually to a recovery or improvement. With TB-I treatment, the disturbances were observed most frequently in the following period; in the Hepatosulphalein test, about in the second to the sixth week; in the cobalt reaction, about in the sixth week. On the other hand, no particular period of disturbances by administration of TB-I was shown in the urobilinogen reaction of urine

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Since several years, I have been studying the flora of the respiratory tract, particularly of tuberculous patients by bronchoscopic examination.
The following are the results of investigation in relation between the secretion obtained by bronchoscopic examinations, sputum of pulmonary tuberculous patients.
1) The flora in the nose and the throat is same as found in the previous stndies except in the same wards a presence of low percentage of streptococcus haemolyticus.
2) A marked, decrease of bacterial flora was noted below the glottis.
3) The number, of flora is so few that it can be counted easily below the trachea.
4) In the trachea the flora was more numerous in cases of tuberculous than those of nontuberculous changes.
5) The percentage of flora is higher in the groups in which were detected tuberculous bacilli in sputum and in trachea.
6) The percentage of flora is higher in the cases in which was confirmed the presence of a cavity in the lung

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By the mutated strains of human type tubercle bacilli, reported previously by us, the aggultination for the tuberculous sera has been tested.
Among a total of 123 sera were 62 of pulmonary tuberculosis, 7 of extrapulmonary tuberculosis, 27 of non tuberculous diseases i. e, rheumatoid, syphilis, pregnancy, 27 of controls with tuberculin positive 15 cases and tuberculin negative 12 cases
The agglutinationtiters were ranging from 0 to 1: 2560. Sera of pulmonary tuberculosis showed on the average titers of 1: 320, of extrapulmonary tuberculosis 1: 80, of non tuberculous diseases 1: 40, and of controls 1: 10.
Titers above 1: 160 were acounted for 88.4% of pulmonary tuberculosis, except severe pulmonary tuberculosis, 14.8% of pregnancy, 28.6% of rheumatoid, 30.8% of syphilis and 15.4% of controls.
An attention should be paid to the fact that sera of severe pulmonary tuberculosis scarcely showed aggultination.
According to our experiments, there is a close correlation between the results of the aggultinationn by the mutated strains of tubercle bacilli and tuberculosis clinically.
This fact is also of significance in the evaluation of the aggultination test as a useful diagnostic procedure in suspected cases and also in prognostication

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To get some aspect of the reserve storage of protein, especially of the mechanism of its mobilization, the extracellular fluid and plasma volume by NaSCN & T₁₈₂₄ were measured in 9 cases of unselected serositis with effusion (Tab. 1), in which the distribution of body water and plasma protein are supposed to change remarkably; simultaneously the electrophoretic protein analysis of plasma and effusion was done and serum potassium & chlor were measured by Kramer-Tisdall and SchaIes & Schales method.
Consecuently, at the begining of the convalescent stage, which is indicated clinically by diuresis and fever-drop, the increase of albumin was noticed with the decrease of extracellular fluid and increase of circulating plasma volume (Tab. 2 & Fig. 1), while, serum potassium increased remarkably at the bigining and decreased later (Tab. 4), And albumin % of effusion is larger than the other fractions and the albumin % of plasma protein in the acme and rather late stadium of the convalescence (Tab. 3).
So in these cases I believe that the source of water and protein in plasma is partly in the intracellular phase, and the change of the distribution of body water is significant for the protein metabolism or protein mobilization. And at that time albumin is supposed to be the most mobilized and utilized figure in the protein fractions

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Before streptomycin and PAS came in to use, we conducted a statiastical review on 75 cases of chronic renal tuberculosis before and after surgical operations and the following conclusions were made:
1) Renal tuberculosis was found mostly between ages 21 to 40 who had previously suffered from tuberculosis of other organs than kidney. Tuberculosis of other organs as well as renal tuberculosis was a part of general tuberculosis.
2) Nothing of special significance was found in the general blood tests in the renal tuberculosis just as in the similar tests for tuberculosis of other organs. Various temperatures were shown, among which the normal temperature was comparatively common larger in number. Unfavorable was the prognosis of those whose temperature rose before and after operation.
3) Fluid out put was slightly impeded and the prognosis of heavier impediments was unfavorable. Excretion of indigocarmin in the incipient renal tuberculosis was slightly prolonged, and that of those who were at in advance condition and in the last stages were markedly prolonged or sometimes stopped.
4) The postoperative prognosis of renal tuberculosis was dependent upon the tuberculosis of the remaining kidney and also of other organs, especially pulmonary and genital tuberculosis.
5) In most cases, vesical troubles were remaining after the operation for a long time and therefore a long time observation was required.
6) Many cases of bone and joint tuberculosis showed a presence of albumin and tubercle bacilli in urine and most of them had renal tuberculosis. The possible relationship between the renal tuberculosis and the bone and joint tuberculosis attracts our attention

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The modification method of Selivanoff's reported by Dr Ando in 1950 was applied to 139 cases of 4th to 10th month of pregnancy
Reagent (A) concentrated HC₆ and, H₂O 4 were mixed and filtered.
Reagent (B) 2% Resorcin solution.
Equal amount of ether and urine are mixed, shaken and is filtered. For several minutes it is immersed in water bath (40°-45°C) and kept warm until no foames produced by shaking. It is kept in room temperature.°CTechnique: a drop of urine treated by ether is taken in the tublet (ca 7cm length., and ca 6cm diameter). 15 drops of reagent (A) and a drop of reagent (B) are added to it and warmed up in the boiling hot water for one minute.
Determination: Light red or pink is SRM (+) which indicates female. Light yellow is SRM (-) which indicates male.
The results of 139 cases which were examined by above method and which became evident after delivery were as follows:
1) The positive rate was 95.4%≥P(+)≥83.1% in male and 83.2%≥P(-)≥67.9% in female with the level of significance in 5%
2) As the reactive substance of this reaction seems to decrease or disappear gradually as the term is approached. Therefore the test should be repeated several times after the 7th or 8th month of pregnancy.
3) A fresh urine; at least within 30 minutes after urination should be used for examination. The positive subetance seems to disappear with the lapse of time.
4) It must be noted that the reaction of the urine may become positive if quinine is given.
5) The result in the case of twin is uncertain.

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While numbers of papers are publicated on variation of staphylococci in vitro, only few was done in vivo previously.
Author has publicated in his second paper the experimental evidence that an antibody-like substanses, which is capable of agglutinating or precipitating the causal microorganisms, is to be proved in the pus, and also the opinion that the variation of staphylococci in host, should be studied from the view point of resistance to antibiotics.
The reason why the staphylococci was taken up as a substrate is due to the facility of distinguishing the color and the hemolysis of the colonies, and moreover to the commonness of this micro-organisms, as a causative agent in the suppurative lesion.
Materials are pus, granulation, and exudate from the abscess, urine, blood, and pleural exudate etc.
These materials from some patients were cultured on the horse blood agar plate from day to day, and in addition to the studies on the number of colonies and the biological characters, the color, hemolysis, mannitol fermentation, plasma coagulation, and the condition of growth on the Löffler's serum slant were observed.
Immunologically, Cowan's slide agglutination on one hand, and the “wetting filter-paper method” measuring growth inhibiting zone in comparison with standard staphylococcus 209 p, was adopted on the other, for the search of penicillin-sensitivity.
In all 97 cases are studied, and the details are summarised as follows:
1) In the rate of 1:3, no change of p-sensitivity of staph. aureus hemolyticus was occured.
2) Definite changes in color and hemolysis without the change of sensitivity to penicillin occured in 1:5.
3) Throughout the course of experiment, gradual decline of the penicillin sensitivity into the confirmed resistance (1000x) were noted in 13 cases, together with change of colors and hemolysis.
4) In two cases, the first p-resistant strain of the staphh aureus hemolyticus changed its sensitivity to penicillin with simultaneous change of color and hemolysis, and the second chan-ged its sensitivity without accompanying the change of color and hemolysis. There is no relation between penicillin treatment and occurence of p-resistant strain.
In 7 cases it was observed the small thin translucent colonies which we called “minute-form”in the mixed or pure culture, these strains were proved to be of a variant of the staphylococci, biologically and immunologically.
This minute-fobm can not be induced by the effect of penicillin, however, the similar variant could be produced from the culture of the minimal-growth-inhibitory-density of streptomycin.
Therefore, the fact, that, as author has pointed out on the general meeting of the 25th Japanese Bacteriological Society, before 3 years, penicillinase producing staphylococci were found very few, in spite of the recent increase of its occurrence; if we assume it as the consequence of the adaptation of staphylococci to the environment, the variation of staphylococcies in viva, should not be catched as the fixed phenomena, but to be studied always as a dynamic feature changing in time itself.
These conclusion iss in accordance with the idea, in respect to the recent currency of the antibiotics-treatment, the study of the variation in vitro or in vivo of the causal microorganism are always to be studied in the correlation of the clinical feature, the idea of which is just the problem of to-morrow

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