The experimental results on rabbits reported previously were that pyelitis and pyelonephritis were easily developed in the case of the antigen-antibody reaction on hosts as the mechanism of the infections, in which those due to E, coli developed by retrograde urinary infections but were difficult to develop by hematogenous infections, whereas those due to staphylococci were just in opposition in relationship.
At this timee the relations between bacilli and infectious tracts were clinically studied on 29 cases of simple pyelitis and pyelonephritis, and 15 cases of stone-pyelonephritis as a contrast.
1) The cases accompanying cystitis, or presenting bladder-symptoms were thought to be the pyelitis or pyelonephritis due to retrograde urinary infections. There were 75.8 96 of retrograde urinary infections, and it rose up to 89.5% on the cases of suspicious retrograde urinary infections.
2) In simple pyelitis and pyelonephritis the isolation rate of E. coli was 68.1% and became 86.3% on adding the other gram negative bacilli. In the cases due to retrograde urinary infections the isolation rate of E. coli was 78.5% and became 85.7% on adding the other gram negative bacilli. In conclusion on the pyelitis andd pyelonephritis due to retrograde infections gram negative bacilli as E. coli were frequent but gram positive cocci as staphylococcus were infrequent.
3) In stone-pyelonephritis due to hematogenous infections the isolation rate of staphylococci was 75.0% and that of enterococci was 18.7%. Gram positive cocci were cultured overwhelmingly numerous.
4) As above these results of the clinical observation were coincided with results of experimental works on rabbits.

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In clinical field, estimation of fibrinogen is important examination for the diagnosis of promyelitic leukemia, hemophilia and hypofibrinogenemia etc.
There are several methods for this assay: 1) turbidimetry, 2) viscosimetry, 3) using formed fibrin clot, a) weighing method, b) colornnetry by biuret reagent3 c) by phenol reagent, d) and by tyrosine reaction, e) ultraviolet absorption method (UV method).
In this present paper author tried to establish ultramicro method of fibrinogen determination using 0.02-0.05ml plasma by ammonium sulfate turbidimetry which is the most simple one among the foregoing methods. These results were compared with those obtaind by specific UV method.
Method
Ammonium sulfate turbidimetry In original method, the precipitant is added to 0.5ml of blood plasma and thus formed turbidity was examined by turbidimetry. In the ultramicro method, all the process was carried out on a scale of 1/10 original method.
UV method
To 1.0ml of plasma, thrombin was added in the presence of buffer solution, thus formed fibrin clot was washed with saline solution and then dissolved in urea solution. This absorbancy was read at 280mu.
Conclusion
The author tried to establish an ultramicro method of ammonia sulfate turbidimetry, and the result was compared with that of UV method which seemed specific. The results are: 1) turbidity is not influenced by the temperature, 2) turbidity should be measured 3 to 6 minutes after an addition of specimen reagent, 3) the result was same by the ultramicro method so far as the proportion of plasma and reagent was not changed, 4) the determinations were compared with UV method in seven clinical cases, and it was found that the latter was 20% less than the former.
Considering from these results, fibrinogen turbidimetry mentioned in the above has some problems in specificity and reproducibility, compared with UV method. However, in a laboratory which is not equipped with ultraviolet adsorption spectrophotometer, fibrinogen assay is available using this method and in addition to this it can be carried out on a ultramicro scale.

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A study were made of diagnosis and treatments of respiratory failure in advanced pulmonary tuberculous patients. Pulmonary function studies (including arterial blood gas determination) and E. K. G. were performed on 130 patients, arterial gas determination and E. K. G. were examined for evidence of respiratory failure in advanced 120 cases. Arterial gaseous tension were compared with ventilation, E. K. G. findings and other clinical symptomes as dyspnea or mental confusionetc.
The results were as follows:
1) Some factors of ventilation, as %VC, FEV 1.0/VC pred, and RV/TLC had a relationship with Pao₂ and Paco₂ level, but FEV 1.0% did not correlated with these parameters. And the cases of %VC lower as 60, or FEV 1.0/VC-pred lower as 40 and RV/TLC over 40 per cent showed abnormal value of arterial gaseous tension. E. K. G. findings also had a close relationship with respiratory failure.
2) Arterial blood gaseous determination were examined before oxygen therapy, and noteworthy facts were the modesty hypoxemia with severity of hypercapnea similar to obstructive lung diseases.
3) It is concluded that low flow oxygen administration with frequent monitoring of arterial blood gaseous is the better method of managing respiratory failure to prevent Cot narcosis in advanced tuberculous patients.

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The Joint Study Unit of Subacute-Myelo-Optico-Neuropathy in national hospitals was organized in 1966, since which time the report of the study for 3 years were already published.
In 1969 the Joint Study Unit for the same project has been reorganized. As the aim of the study at this time, the epidemiological and pathogenetic aspects are most stressed on. In the clinical section, the treatment and the follow-up study are encouraged among 22 institutions with use of a due form.
The total cases presented from the all study units in 1969 was 163, and they were classified into group A (definite cases), group B (suspecious cases) and group C (ruled out cases).
The results of the study are as follows The report from the epidemiological section
1. In the both A and B groups, it occurs in the female two to three times as frequent as in the male sex and preponderant over the fourth decades.
2. Often there is a history of herpes zoster or herpes labialis in past.
3. Often they have predilection of the upper respiratory infection.
4. There is no special relationship with residence or food. The report from the clinical section
1. 64.3% of cases were improved with sequelae, 15.6% of cases were neither recovered nor worse and 13.8% of cases were suffered from remissions and exacerbations.
2. Over 60% of cases were improved by either administration of adreno-cortico steroid hormons, vitamin B groups or A. T. P. -nicotinic acid I. V. drip.
3. On follow-up study, most cases improved within one and a half years although some cases were improved over two years after the onset of illness.
The report from the bacteriological and serological sections
1. The microecological studies on the fecal specimens of 11 SMON patients were carried out. E. coli, Enterococcus, Lactobacillus, Staphylococcus epidermidis and Candida were isolated from the fecal specimens of every SMON patients. The bacterial numbers of E. coli and Lactobacillus isolated from fecal specimens represented variability between 10⁷-10⁹ viable cells The bacterial numbers of Staphylococcus epidermidis varied considerably between 10¹-10⁶ viable cells per fecal pellet and Candida did between 10²-10⁴ viable cells. Salmonella meleagridis was isolated from one SMON patient which died because of complicating encephalopathy.
2. The agglutinating antibody titers against Salmonelle meleagridis appearing in the sera derived from SMON patients were much higher than those appearing in sera obtained from normal healthy individuals and non-SMON patients.
Furthermore, the increase of IgG was observed in one patient's serum and the decrease of IgG was observed in two patient's sera as the results of the quantitative estimation of immunoglobulins on SMON patients' sera employed here.
The report from the virological section
1. Total clinical specimens were 40 examples composing of A group 34, B group 3 and C group 3.
2. Complement fixation reaction was carried out in most of sera derived from SMON patients and neutralization test was done in some of the sera.
3. There were no elevation of antibody titers in 18 paired sera tested.
4. None of viruses were isolated from feces, spinal fluid and pharyngeal slime.
The report from the pathological section In the past one year there is two postmortem examinations, which correspond with the patho-histological findings of the already reported cases of SMON, i. e.: the lesions are predominant in the long tract of the spinal cord and prominent in the distal portion. They reveal continuous symmetrical degeneration of axones and some invade even nerve roots, peripheral nerves or optic nerves. As regards the degenerative changes of the nerve fibers, some shows marked disintegration of the myelin sheath and preservation of the axis cylinder, the others show swelling, tortuousity or disappearance of axons with preservation of myelin sheath, thus the p

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A case of primary tumor from The greater omentum was presented. The patient, a 53-years-old male was admitted in our department with a sudden abdominal pain in Dec., 1967. He had had lung tuberculosis in 1959 and pneumonia in 1963. He had a child's head sized, movable, elastic hard tumor in the right flank.
As the result of several physical examinations, the tumor was a greater omentum tumor, which was removed soon. The weight was 1270 gm and the color was darkred. Histologically, it was diagnosed as angio-endothelioma of the greater omentum.

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Dasen, an anti-inflammatory enzyme preparation, was given orally prior to the X-ray examination of stomach and its effect was comparatively studied. The following were the result
1. A group, to which Dasen was given for 2-3 days, and the other, to which Dasen was given for 4-7 days, were compared with the coiltrol group to which Dasen was not given. In the former, any difference from the control group was not noted in respect to the spreading on the gastric wall and foaming of barium. In the latter, however, more favorable results than those in the control group in respect to both points were obtained.
2. In the X-ray pictures taken before and after the administration of Dasen on the same patient, foaming of barium several minutes after its intake was much less after Dasen and a favorable result was obtained as a whole, though any statistically significant difference was not noted between the films in respect to the spreading and foaming of barium immediately after its intake.
3. In the gastrocamera pictures taken on three cases after Dasen, disappearance of gastric mucus, inflammation and diet residua was noted.
4. The complication was none in the all cases.
5. Improvement noted in the X-ray films can be attributed to the anti-inflammatory and mucolytic activity of Dasen. It is useful, therefore, as a measure or obtaining fine X-ray pictures of the stomach.

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