The Joint Research Committee on the Occupational Therapy, participated by 23 National Sanatoria, made cultivation of sputa, once in a week, for 24 weeks, from 347 cases of recovering tuberculosis, who were under occupational therapy, and investigated the occurrences of positive culture and the number of colony of tuberclc bacilli, and observed the relationship between the obtained data and the clinical relapses during the course of occupational therapy.
In the 323 cases which had no clinical relapses, the following data were obtained:
a) About 10% of them gave positive cultures.
b) In more than 60% of the positive culture cases, number of colony counted was less than 10.
(figures were the sum total of the colonies developed in the two test-tubes, in which 0.1cc of sputa diluted twofold by 8% of NaOH were cultured)
c) Cases of which cultures gave any positive results during the whole period of 24 weeks were about 50% of the total cases, and about half of the said cases gave less than 10 colonies, while other 15% of them gave more than 100 colonies.
d) Generally, the smaller the maximum number of colonies, the lower the frequency of positive cultures, i. e., in 79 cases of which maximum number of colonies developed were less then 10, only 38 cases gave positive cultures by every four weeks' examination.
In the 24 cases which had any clinical symptom of relapses, the following data were obtained:
a) More than 40% of them gave positive cultures.
b) In more than 80% of the positive culture cases, the counted number of their colonies was over 10.
c) Except 1 case, the relapse cases gave positive results and deveioped more than 10 colonies, The maximum number of colonies throughout the 24 weeks was 11 to 20 in 2 cases, while the other 21 cases developed more than 100 colonies.
d) Generally, 1/2 to 5 months preceding the appearance of clinical symptom of relapses, they showed more than 40 colonies in their cultivation.
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