Immediately after the occurance of paedonephritis investigation of blood picture, blood pressure, inorganic phosphate, protein-serum protein fraction in some patients-, cholesterin, non-protein nitrogen values, cobalt, cadmium, lugolreactions were undertaken. The findings of these tests were compared with the three disease groups which were describedin the previous report.
Group I-acute type of glomerular nephritis, group II-subacute type of glomerular nephritis and group III- chronic type with nephrotic syndroms.
1) The incidence of raised serum inorganic phosphate value presumes tunular insufficiency. In the early stage of the disease this rise was noticed equally among the three groups. And so this rise did not enable us to predict the prognosis of each cases.
2) Thepatients with the following positive reactions or medical findings had tendencies to have chronic process; abnormal left deviation of Co-R and Cd-R, Lugol reaction positive, hypoproteinanaemia, hypercholesterinanaemia, high grade anaemia, and hypereosinophlia.
3) In the early stage of the disease patients with highly raised blood pressure were found mostly among group III, while patients with raised serum non-protein nitrogen values were seen among Group I and II. And patients who had the onsets with uraemic syndroms were mostly found among Group I.

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On six women and eight dogs, EEG, ECG and respiratory curve were simultaneously recorded during trichiorethylene anesthesia. At the same time, the concentration of trichlorethylene in blood was measured. The obtained results are as the following:
1) On the human subjects, during the anesthesia, the EEG were supressed in the begining and then the amplitude increased and the basic cycle declined to slow in proportion to the increase of the concentration of trichlorethylene in blood: i. e. from 10∼8c/s, into 8∼6c/s, 6∼4c/s and 4∼2c/s.
The frequencies of the heart beat and the respiration increased until the basic cycle of EEG indicated 8∼6c/s. Afterwards the frequencies were sustained “unchanged” in spite of the deeper anesthesia. In the process of the anesthesia no noticeable findings were observed.
2) On the experiments among dogs, one out of the eight dogs died during inhalation of trichlorethylene over one hour. Paralleling the anesthesia, the cycle of EEG became slow and heart beat and respiration became frequent. The concentration of trichlorethylene in blood increased over 20mg/dl at the final stage. In the fatal case it was measured over 30mg/cl.
3) The optimum depth of trichlorethylene anesthesia was when the frequency of EEH indicated 8∼6c/s, and the concentration of trichlorethylene in blood at the time showed 5.5 to 8.5mg/dl.

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Forty three cases of brain injury in the World War II (1937-1945), in whom the alien substances (splinters of bullet or shell, or fragments of bone) remain in the brain substances, were followed 10-20 years.
1) The general conditions after 10-20 years of injury were as follows: no change 28.2%, to get worse by works 18.7%, sometimes worse and sometimes better 20.3% very much worse 4.7%, and the better in no cases. Six cases died in ten years, therefore, the death rate was higher than that of general cases of brain injury during the war.
2) Frequently subjective symptoms were noticed among them such as; amnesia, head heaviness, headache, feeling muddle-brained, dizziness, chilly of hands and feet, irritability, disturbance of thinking, fatigue, stiff shoulder, easy fatigability. Those symptoms remained existing in over 50% of cases.
3) Changes of personality, decrease of spontaneity, disturbance of will, emotional instability, lack of confidence, unsociability were noticed in many cases.
4) The epiletic seizures occured in 57.2% of cases (grand mal 30% and peptic mal 27.2%). This rate was increased 22% more than the rate of shortly the wounds.

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In the first report the author described the body weight and the nutritive methods with the non-TB infected mice. The similar nutritive methods were applied at this study and the applied factors were the same; DD strain mice, Aoyama bacillus, streptomycin and INH.
Results are as follow:
1) TB infected mice were fed by 5 methods. Death rate was remarkably high among a group of mice nourished by low protein. The fat was needed, however, the protein was found to be more important to live.
2) When infected mice are treated by INH, increase of body weight is noticed among the mice which are fed with high protein. On the contrary, decrease of the body weight is seen among the mice which are feed with low protein and high fat diet.
3) During the period of chemotherapy, the relation between the nutritive method and the total bacillus quantity in the lung, liver and spleen of the mouse was examined. However, no remarkable difference was noticed. Growing colony in lung was less in case of SM treatment than of INH treatment.
4) When TB bacillus was inoculated in the tail vein, tubercle grew mostly in the lung. But the appearance of this tubercle showed no difference between the mice with high and low protein diet.

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We stated on our first report that a leprosy patients was in the state of slight acidosis, and now we made observations on its cause as well as remedy. Acid-base balance is said that it has a relation especially to protein metabolism in a liver.
We made comparative studies with serum iodate level and serum buffer level, which were convenient to find out an outline of intermediary protein metabolism.
Furthermore, administering a leprosy patients vitamin B₁ to let intermediary protein metabolism be smooth, we observed its effect on intermediary protein metabolism and acid-base balance.
We used Eguchi's transfigured method for serum buffer level and Nishigaki's transfigured method for serum iodate level, and got the following results:
1) Serum iodate level in leprosy patients showed high degree of neural type and lepromatous type in order, compared with those of healthy men.
2) For the degrees of the diseases, neural type stood between moderate and serious cases, whereas lepromatous type showed a difference between slight and serious cases.
3) For a relation of serum buffer level to serum iodate level, serum buffer level showed low degree at a healthy man, neural type and lepromatous type in order, but serum iodate level showed on the contrary high degree and the order was found reversely.
4) For an effect of administering vitamin B₁ on serum buffer and iodate levels, the neural type seemed to be improved remarkably after injection, bat in case of lepromatous type, the degree of improvement was less than that of neural type.
According to this study, we recognized that the most part of fluctuation at serum buffer level in leprosy patients was related to protein metabolism, and the same metabolism was concerned with a lack of vitamin B₁.

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1) Using Mycobacterium avium as the experimental material, careful reevaluation of the various fractional quantative measuring methods for the nucleic acids (RNA, DNA, etc.) such as the Ogur-Rosen, the Schmidt Thannbauser, or the Schneider method revealed these methods to be inadequate for certain specific purposes.
This report deals with these inadequacies and the reasons that the above mentional methods do not meet the requirements.
2) Measurement of the Ba-Fraction of the acid-soluble phosphates revealed no difference between the SM-sensitive fraction, and SM-insensitive one.
3) In the quantitative analysis of the Nucleo acids of the SM-sensitive Mycobacterium avium and the SM-insensitive bacilli, no considerable effects of the SM on the contents of RNA and DNA could be found.
4) It was proven that RNA was extracted from Mycobacterium avium, by being tracted with TCA for many hours at room-temperature. Mixing of protein and DNA could not beobserved. From the results o4the quantative analyses performed, it may be stated that the RNA content, the ratio of the bases of RNA, the phosphatic acids and pentoses, the solubility of RNA, etc., were not effected by SM.
5) Ecteola cellulose, when used as exchanger, makes it possible simultaneously to fractionate AMP, adenyl pyrophosphate, inorganic Orthophosphate, inorganic Pyrophosphate inorganic Polyphosphate, high-molecular RNA and others by means of column chromatography. Even with this method, no noticeable effect of SM on the various groups of the phosphatic acid compounds could be observed.
6) Taking ³²P's specific activity into account, the incorporation of ³²P, used as a tracer, at room-temperature, with RNA by means of a TCA extraction was studied.
In the case of sensitive bacilli, the incorporation of ³²P RNA was inhibited by SM, this conclusion being drawn from tests conducted on RNA which was extracted 24, 72 and 96 hours later.
In this test, in the bacilli treated with SM for 2 hours, a strong inhibition of RNA was observed, while no effect was found on DNA.
Even after treating the bacilli with SM for 12 hours, still no inhibiting effect on DNA could be noticed.
On the other hand, the insensitive bacilli schowed no inhibiting effect either RNA or DNA.
7) After detracting TCA from the material extracted from RNA by means of TCA extraction, this material, using Dowex 1 (X2) and employing the formic acid group as the reagent, was tested through the use of column chromatography, a complex migration pattern consiting of various Olyonucleotides mixed with mononucleotides.
SM also inhibited the incorporation of ³²P with these nucleotides.
8) The sudy of sensitive bacilli with ³²P as a tracer revealed the inhibiting effect of SM on the synthesis of polyphosphates.
On insensitive bacilli, SM showed no such effect.
9) With respect to the inhibition by the incorporation of ³²P with cytdylic acid. Adenylic acid and polyphosphates of RNA, the results of this study were as follows a) The bacilli suspended in a Sauton media, which state may be considered as being an ideal physiological condition, showed the remarkable rate of inhibition of 20%.
b) The inhibition, on the bacilli suspended in a buffer solution of phosphoric acidd attained a rate of from 33 to 34%.
c) The inhibition hardly could be observed in the bacilli suspended in water and the maintenance-metabolism of the bacilli-cellule was not suppressed by the SM.
Specifically, SM impedes the bio-synthesis of RNA and the phosphates, not the more exchange reaction.

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