We made some improvements on the Aika Gebauer Twin Soirometer Bronchospirography, manufactured by Ichikawa Co. Ltd., so that a tidal air gramme curves can be recorded and magnified almost horizontally (see Fig. 2). A special flexible catheter was also made (see Fig. 1), by knitting silk threads coated with binyle coverings, in order to reject the defects of the custommally used catheter which is made of metal or rubber.
By means of the above mentioned apparatuses, we recorded the tidal air gramme curves of both lungs simultaneously in healthy person and many patients, to reveal the real conditions of pulmonal functions, regarding both time and quantity. The following results were obtained:
(1) The tidal air gramme curves of each lung of healthy male adults showed an approximately convex type, however, the curves for the part of aspiration period were somewhat longer than that of expiration period. The heights of the both curves, (left-and right-sides) were almost equal to each other, which means that the vital capacity, complemental air and supplemental air, for both lungs, are almost equal altogether.
(2) For the patients who had thoraco-plasty, the recorded curves were remarkably low in the operated lung, and the curve of the operation-free lung was a little lower than that of healthy person. This means that operation on any side of lung will lower the pulmonal functions of the opposite side.
(3) The tidal air, vital capacity, complemental air, and especially supplemental air volumes decreased in the lung of operated side.
(4) In operated lung, the number of the deflections of the curve per minute was larger than that of healthy cases, and the heights of each deflections were irregular.
(5) As for the patients, who were under pneumo-thorax therapy and pneumoperitoneum therapy, the tidal air was low and the vital capacities for both lungs were almost equal.
(6) In the cases who had plastic-ball prombage, there were no remarkable differences in the tidal air gramme curves of both lungs.
(7) In the patients who were under thoraco-plasty or who had remarkable adhesions in one lung, the deflections of curve showed a swing towards the left, while, in cases whose diaphragms were out of order, or who were under pneumoperitoneum therapy, the curve showed a swing to the right side.
(8) The ratio of CQ₂ divided by O₂ of suffered lungs, which were under thoraco-plasty, and that ratio of those which had serious pleural thicknings or bronchiectasis, were generally larger than that of the lungs of the opposite, non-suffered side.

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Hemagglutination reaction described by Middlebrook and Dubos were examined on the sera from 271 in patients at the Obihiro National Sanatorium, using Denden's old tuberculine as an antigen and the human group 4 erythrocytes as that one which is sensitized. The obtained results were statistically analyzed, principally referring to clinical symptoms, types and conditions of the disease. The results are summarized in the following:
1) Of 271 patients, 2 were free from TB, 237 were pulmonary TB, 12 were of tuberculosis of other organs and 20 were almost recovered cases.
2) Of 269 cases of tuberculosis, 98.8% were positive at the agglutination titer of 1:7, 88.1% were 1:14 and the average agglutination titer of all cases was 46.8.
3) When 269 cases were divided into four groups of severe, moderate, slight and arrested group, according to their clinical symptoms, average agglutination titers of these groups were 77.7-43.7- 20.6 and 9.8, respectively.
4) When they were divided into four groups of high fever, slight fever, intermittent slight fever and normal fever group, the average agglutination titers of these groups were 86.9-57.2-22.4- and 38.8, respectively.
5) 4 groups of the total cases divided according to the amount of sputa; i. e., large amount group, daily 30 to 50cc group, daily 20 to 30cc group, daily around 10cc group and scarce expectoration group gave, respectively, the average agglutination titers of 82.0-105.0-42.6 and 47.2.
6) 269 cases were divided into five groups of Gaffky 7 to 10, Gaffky 4 to 6, Gaffky 1 to 3, culture positive and culture negative group, according to the secretion degree of tubercle bacilli in the sputa. The average agglutination titers of these groups were 83.1-61.1-80.0-46.8 and 30.4, respectively.
7) Three groups of the cases divided according to their blood sedimentation rates; i. e., over 31mm/hour, 16 to 30mm/hour and 0 to 15mm/hour, gave 71.1-85.0 and 22.1 of average agglutination titers, respectively.
8) We could not find any particular relationship between the agglutination titers and the intensity of Mantoux reaction.
9) When the 269 cases were divided into five groups of exsudative, productive, mixed, cirrhotic and arrested group, according to their type and grade of the disease, the average agglutination titers of these groups were of 79.9-34.4-31.8-21.9 and 9.8 respectively.
10) It seems that the higer titers are generally found more in their number among cases having wider infiltration lesions in the lungs.
11) No special correlation was found between the agglutination titers and the existence of TB cavity in the lungs.
We observed that the intensity of the agglutination titers have some correlation with the clinical symptoms, i. e., fever, blood sedimentation rates, etc. These facts make us believe that hemagglutination reaction will reveal, to some extents, the progress of tuberculosis.

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Lately the problem of so-called pathogenic coil group has been discussed in the light of pathological agent of infantile diarrhea. Fukumi and others already reported their studies on this subject and Braun gave general study on ‘Dyspepsiecolibakterien’. We investigated certain types of E. coil, O-111, O-55 and O-26, in the infants at the nursery, pediatrics wards, outpatient clinic of the 1st National Hospital of Tokyo and at four other nurseries. Clinical symptoms of these cases, the carriers of the above mentioned types of E. colt and the relationship between the excretion of the bacilli and diarrhea were jointly studied.
Out of the total of 693 cases investigated, we found 3 positive cases, of which one case was a carrier of O-55 and 2 were carriers of O-111. The distribution of the bacteria was not wide. It was clear that within a closed environment such as nursery ward, the bacilli were disseminated comparatively rapidly and that there is some close relationship between the findings of the bacilli and the onset of diarrhea.
The geographical distribution of the bacilli is not wide, However, there is the chance of ‘epidemy’, if conditions are agreeable with the spreading the bacilli. Incidentally, it is interesting that O-111 were found from the pillow of an infant in the ward.

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Prof. Yasuda and Dr. Shirai et al. proved that, within the serum of rabbits to which the continuous injection of chorionical gonado-tropic hormones, trophoblast hormones, or pituitary gonado-tropic hormones had been administered, antihormones antagonizing against these hormones were produced.
We discussed lately the two identifying methods of antihormones, which are produced by the formation of bloody folli culus ovarii and corpus luteum of rabbits, in order to find the production, of antihormones and its fluctuation, and we named them the antihormones reactions.
By means of the 2nd antihormone reaction, the author investigated the fluctuation of antihormones after discontinuance of the injection, in the rabbits to which trophoblast-hormones (pregnant women's urine, watery human placentin) had been injected continuously.
1) In case trophoblast-hormones (pregnant women's urine, watery human placentin) were injected continuously, an antagonizing substance (antihormones) was produced within the rabbits blood. The above mentioned antihormones were produced regardless of the sex; i. e., it was not necessary for the production of antihormones to be the possessor of ovaries.
2) As the continuous injections of very small amount of pregnant women's urine are necessary to produce antihormones in rabbits, it is possible to presume the presence of hormones within the injected substance if antihormones are identified in rabbits. As the results of the chemical investigation for the gonado-tropic hormones from healthy unpregnant women's urine and healthy men's urine by means of this method, the existence of antihormones against trophoblast-hormones was not proved in them.
3) It was recognized that the fluctuation of antihormones in the rabbits blood by discontinuance of successive injections of trophoblast-hormones (watery human placentin) had a close connection with the length of period of the successive injections; i. e., the longer the period of injections is, the longer the existence of antihormones lasts.
Accordingly, if the injectjon of hormones is continued after the production of antihormones in the rabbits' blood, it will delay the disappearance of antihormones after discontinuance of the injections.

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We investigated the synergistic effects of INAH and other antituberculous drugs, regarding their bacteriostatic and bacteriocidal reactions in vitro.
1) The bacteriostatically synergistic effects of Dihydrostreptomycin, PAS, TB1, Vitamin K and Z-Furan, respectively combined with INAH were proved. Vitamin K in the combination of INAH was the only one which showed, though slightly, the bacteriocidally synergistic effects.
2) In the combinations of INAH with the two of the following drugs; Dihydrostreptomycin, PAS and TB₁; the combination of INAH, Dihydrostreptomycin and PAS was the most effective one, having the strongest bacteriocidal activity. Other combinations were of weaker effects, less effective compared to the combination of INAH and Dihydrostreptomycin; INAH and Vitamin K; and had no effects upon bacteriocidal activity.
3) The combination of INAH, Dihydrostreppomycin, PAS and TBI was not more effective than that of INAH, Dihydrostreptomycin and PAS.
4) Against INAH-resistant bacilli, the combination of INAH, Dihydrostreptomycin and PAS, more or less, increased the original antimicrobic activity of INAH, while the combination of INAH, Dihydrostreptomycin, PAS and TB₁ decreased the original antimicrobic strength of INAH.
5) Against Dihydrotoreptomycin-resistant bacilli, the combination of Dihydrostreptomycin and INAH did not increase the antimicrobic activity of Dihydrostreptomycin.

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