Since Sasaki, Yoshida and Kinoshita and others reported the experimental liver cancer induced by azo dyes, studies on the experimental cancer, especially factors affecting its development, made a great progress in our country. The author conducted experiments to measure the catalase activity of liver of rats fed with o-A-T and injected with Methyl-thiouracil (M-T), in order to find the effect of thyroid upon the development of liver cancer.
As for the experimental animals, male rats weighed around 100g were used. As for cancerogenics, 0.1cc of 5% o-A-T solution in olive oil was given daily through a metal catheter, while 0.5cc of 2% M-T solution was injected 3 times a week. For the control, rats fed with o-A-T only, or injected with 2% and 10% solution of M-T only, or non treatment rats were used. Catalase activity was measured according to the Seneter's method, calculating the unresolved H₂O₂ after the end of catalase action of liver Results obtained were as follows:
1) Catalase activity of liver of normal rats was 9.61±1.85 in the average.
2) Catalase activity of liver of rats fed with o-A-T decreased very showly for 300 days and then it became quickly zero level.
3) Catalase activity of liver of rats injected peritcneally with 0.5cc of 2% M-T, 3 times a week was 6.09-8.87, and that of rats injected with 0.3cc or 0.4cc of 10% M-T for 3 or 4 days was 5.02-5.96.
4) Catalase activity of liver of rats fed daily with o-A-T and injected peritcneally 3 times a week with 0.5cc of 2% M-T was as shown in the table:
Catalase activity of liver of rats fed with azo-dye in hypothyroid state was lower than that of rats fed with o-A-T in normal thyroid state and both decreased gradually, and by 300 days of experiments the latter became immediately zero level, while the former remained at 4.00 on 360th experimental day.
Many investigators have certified the decrease of catalase activity of human cancerous tissue. It was Blumenthal who found that fact for the first time. Meanwhile, Nakahara, Yanagisawa, Miyachi and Greenstein and others assured experimentally the close relationship btween catalase activity and cancer, i. e. its decrease in cancerous tissue and its increase by removal of the cancer. The author examined the effects of thiouracil, antithyroid drug, upon the dvelcpment of liver cancer, measuring the catalase of liver and was able to prove the counteraction of thiouracil to the development of the liver cancer.

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A follow-up study was made on 577 TB cases who had been under occupational care therapy and discharged from the National Sanatorium Muramatsu Seiranso during the period of 1939 to 1953.
Co-relation between the post-discharge physical status and occupational status and predischarge physical status was analyzed. Results were as follows:
1) Cut of 577 cases studied, 58 (10.1%) died from tuberculosis
2) The rest of the cases were currently engaged in a variety of occupations, such as farming, office and clerical jobs, retail business, and x-ray and laboratory technicians.
3) Among the vocational trainings, x-ray technique and laboratory technique were chosen by many patients after their discharge from the sanatorium. Although at the time of their discharge they were not always minimal cases, their mortality rate was considerably low. It is considered that these occupations are suitable for the ex-patients, because they may be under constant medical supervision.
4) Relapse rate of ex-patients who took agricultural works was not so high. Agricultural works may be well tolerated by patients with minimal lesions or thcse who had sufficient ventilatory reserve after thoracoplasty treatment.
5) It was generally recognized in all works that relapse was related to the pre-discharge physical status. Therefore, effective therapy must be instituted before discharge from sanatorium or hospital.

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Since the thesis of General Adaptation Syndren e was first presented by Selye, it has been noted that adrenal cortex plays one of the most important roles in the cases of stress. In order to investigate the adrenal cortical function in tuberculosis, 131 pulmorary tuleroulosis cases, hospitalized in Kobe National Sanatorium during the period from November 1952 to September 1953, were examined and divided into two groups for investigation objects. While the first group of 86 cases was submitted to 0.3 epinephrine test, the second group of 45 cases was submitted to intravenous ACTH test, by two-divided dosis. The function of adrenal ortex was examined by the above tests and others items were observed in relation to clinical courses. Results obtained were as follows:
1) Most serious cases of pulmonary tuberculosis showed poor eosinopenic response of below 50%: cases having fever, far advanced lesions through x-ray examination and complication of intestinal tuberculosis showed insufficient eosinopenic response.
2) It was noted that the eosinopenic response in ACTH was more closely related to the clinical symptoms, with consistency, than in case of epinephrine. In other words, ACTH may be more useful than epinephrine. Upon these results, it may be considered that very serious cases of pulmonary tuberculosis show low eosinopenic response (less than 50%) and develope disfunction of adrenocortex. The adrenarine method in this view might be not so good method and the data of eosinopenic response (50%) might he doubtful data. Incidentally, it is no doubt that the ACTH method is superior to the adrenarine method and concerning to ACTH injection, there are intramscular, intravenous and drop methods. In conclusion, it is slated that the very severe cases of pulmonary tuberculosis show low eosinopenic response (less than 50%) which may be the sign of disfunction of adrenocortex.

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In order to measure quickly the sensitivity of tubercle bacilli against antituberculous agents in slide culture, the author made a judge method to determine the population of sensitivity for antituberculous agents.
1) Smear the sputum to be examined on the edge of a object glass, which was previously cut half longitudinary. The smear is dried.
2) The smear is dipped into 6% H₂ SO₄ solution for 20 minutes.
3) Then it is passed through sterilized destined water for 1 to 2 minutes, two times.
4) Meanwhile, put the antituberculous agents of various concentrations into Kirchner's liquid medium, into which malachit-green has been added.
5) Cultivation shall be conducted at 37 degree C for 7 days.
6) The subject is dried, fixed and stained by Ziehl-Neelsen's Method.
7) Examine under microscope.
Counts of separated bacilli and mass of bacilli were classified into six groups: 1, 2 to 5, 6 to 10, 11 to 20, 21 to 30, more than 31 bacilli. Percentage of frequency distribution in each group, percentage in the liquid media, into which various concentrations of antituberculous agents were added, were compared with that of non-inoculation or media without any agents. In case of cultivation, differences came from the following two points:
1) Crush the strains incubated for about two weeks; add 50% to 100% horse serum to it; centrifuge at 2, 000 r-p-m for five minutes; prepare bacilli suspension which shall be equal bacilli counts of 4 to 7 Gaffky number; and staining.
2) Cultivation at 37 degree C for 10 to 14 days. Then the results of sensitivity for INAH according to the author's method were compared with that of Ogawa's method, in case of bacilli from sputum and that of Kirchner's culture method, in case of bacilli treated by cultivation.
Generally, the grades of sensitivity of tubercule bacilli against INAH in slide culture method were proportional to that of Ogawa's method. The results were supplemented by microscopic examination of low magnitude in addition to high magnitude examination.
In case of bacilli treated with cultivation, the grade of sesitivity against the agents were equal in three methods, i. e., slide culture method and Kirchner's culture method.
Generally, population of sensitivity of tubercule bacilli against antituberculous agents, especially INAH, in slide culture method, was proportional to that of Ogawa's method, however, the former showed higher level.

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Leprotic foot-drop is very important problem in the treatment of malum perforans leprosum. If malum perforans leprosum is complicated with lehrotic foot-drop in the same foot, it is very difficult to treat that malum perforans. The authors made a statistic observation and treatment of leprotic foot-drops.
Results; A) Statistic observation: Statistics was taken from 1058 cases in the lepresarium, as of October 1953.
1) Number of foot-drops was 194.
2) Numer of foot-drops in lepromatus type was 107, which was 15.13% of all the lepromatous type inpatients in the leprosarium. Number of cases of foot-drop in the reural type cases was 87, which was 31.98% of all the neural type inpatients in the leprccariurn. Significant statistical difference in the frequency of foot-drop is found between these two tyres.
3) In both types, the grades of neurological disorders were in proportion to the frequency of foot-drop cases.
4) The period from the development of foot-drop to the time of the observation in neural type cases was longer than in lepromatus type cases.
B) Prodrome and progress of foot-drop: As for the prodrome or the complication fcr the foot-drop, neuralgia was numbered in the first place.
There are two types of foot-drop, temporary and permanent ones.
C) Relation between the foot-drop and the malum perforans; Malum perforans leprosum complicated with foot-drop was located in toes, in many cases. This may be understood as that the toes are pressed abnormaly and irregularly when stnding or walking in foot-drop cases.
D) Treatments: Conservative therapy, such as massage or splint application, may be good in the treatment of the foot-drop in its earliest stage, especeally, for the temporary foot-drop cases, Operation (arthrorisis or arthrodesis) may be best treatment for the permanent foot-drop cases, and after the operation, reforming slices shall be used, if possible. Tenodesis may not be effective. Incidentally, there were cases, which developed foot-drop without prodrome and with slight grade of distorsion of foot joint only. Those cases had paralysis of peroreal nerve before the development of foot-drop. That status may be called as the latent foot-drop stage Finding the latent foot-drop may be a pretventive measure of foot-drop.

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The suthors has reported a part of the present study at the 38th and 39th meetings of the Japanese Gastro-enterological Society. In that report, the authors asserted that the hook worm must be divided into two classes, i. e., Necator americanus and Ancylcstoma Dubini. In this paper, several items such as changes of gastric juice, correlation between gastric juice, anemia, worm excretion and pituitary adreno-cortical system, and consequence of anthelmin treatment.
20 cases diagnosed as ancylostomiasis Dubini were investigated reagrding to their gastric juice obtained by the Katsch-Kalk's method. They were also examined with respect to blood picture and pituitary-adrenc-cortical system's function. For the anthelmin, tetra-chlor-aethylen was administered 2 or 3 series at the intervals of four days. The worms were examined by all feces of all cases. Complete excretion of worm was judged by the suspension method and culture method which were employed three times at the second week from the last anthelmin administration.
Achylia was found in 13 cases and hypo chylia in 3 cases. As for total acidity, there were 12 cases of hypo-chylia and 8 normal cases. As for maximum free acidity by the administration of the medicine, there were 7 cases of hypo-chylia, 8 normal cases and 5 cases of hyper-chylia. As for the time of dye excretion, there were 12 normal cases, 6 cases of prcmptitude and 6 cases of delayed excretion As for acidulous curve, there were 7 cases of normal curve, 6 cases of flat curve, 4 cases of delay curve, 3 cases of accelerated curve and no cases of continuous curve or gradual curve. There were many cases of hypo-acidity without any relation to the degree of their anemia. This may be derived from the fact that most of the cases were anemia of slight degree. In cases of slight anemia, the maximum free acidity was normal level or greater by the injection. There were no relations between anemia and the time to reach at the maximum acidity or the acidulous curve. Incidentally, in case of slight anemia, time of dye excretion was generally delayed. There were no relation between worm excretion and hunger acidity, maximum acidity, and the time to reach the maximum acidity. No relation were found between worm excretion and acidulous curve. Cases of which ecsinophil cell increase was greater than 11%, had low acidity. There were no relation between % of eosinophil cell and the time for excretion or acidity curve. No distinct correlations were found between pituitary adreno-cortical system function and gastric juice.
As for the effects of anthelmin, 4 cases showed increase of acidity, 8 cases showed more or less favorable outcome. 4 cases showed low acidity by anthelmin and they were more than 45 of age. Relation between the acidity and anemia was not distinct. The last result will not agree with the former study. It is supposed that hydo-acidity may be seen in case of remarkable hypereosinophilia. Result of Thorn's test gave no relationship between acidity and pituitary-adreno-cortical system. It is supposed that not only worm excretion but also age, term of infection, disposition etc. are related to the degree of acidity.

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Experimental study was conducted on the effects of intramuscular injection of cow-milk (5cc, verey other day) in normal male white rabbits, and the following results were obtained.
1. Subsequent to the initial injection, the Indian ink particle phagocytosis of pseudoeosiophile cells were accele. ated in every case. Generally, the phagccytosis showed off temporarily by the following day of he second injection, and the maximum phagccytosis was observed by the 2nd-4th day of the second injection. The accelerated phagocytosis gradually fell off to the original level by 10 days after the 2nd injection.
2. The amount of serum globulin increased gradually subsequent to the 1st and 2nd injection. It showed the maximum level by 1st-4th day after the 2nd injection and gradually fell off to the original level by around 13 days.
3. The rate of pseudo-eosincphile cell became the highest figure by the 1st day subsequent to the 1st injection. It temporarily became at the original figure by 3rd-6th day and it was at that figure or smaller level during the 15 days of the experiment.
4. As for nuclear shift of the pseudo-eosinophile cells, left shift was observed subsequent to the 1st injection and more left shift was observed subsequent to the 2nd injection. In around 2 weeks, nuclear shift recovered.
5. Concentration of acid fuccin in the aqua anterior decreased by 1 week after the 2nd injection and slightly increased during the following 2 weeks.
6. Preliminary immunization, by means of dead coli bacilli, was given to rabbits, to which cow-milk injection was conducted 7 weeks after the in munization. The agglutinin value of serum increaed by 1 week, however, that of aqua anterior did not. The production of aggultinin was remarkably restrained, in case of the concommitant performance of the immunization and the milk injection.
7. Body weights were intact, practically. Body temperature became slightly high degree, subsequent to the 1st and 2nd injections.

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