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Volume 28, Issue 7
Displaying 1-11 of 11 articles from this issue
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Article type: Cover
1979 Volume 28 Issue 7 Pages Cover28-
Published: July 30, 1979
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (51K) -
Article type: Cover
1979 Volume 28 Issue 7 Pages Cover29-
Published: July 30, 1979
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (51K) -
Article type: Bibliography
1979 Volume 28 Issue 7 Pages Misc8-
Published: July 30, 1979
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (238K) -
Yukinobu Ichikawa, Masatoshi Takaya, Shigeru ArimoriArticle type: Article
1979 Volume 28 Issue 7 Pages 559-567
Published: July 30, 1979
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSWe investigated ADCC of human Peripheral blood leukocytes by using ^<51>Cr-labelled sheep red blood cells(SRBC)as target cells and anti-SRBC rabbit antibody. Lysis of SRBC was mediated by either human peripheral lymphoid cells or phagocytes(monocytes and granulocytes). SRBC were useful as target cells in ADCC assay against human lymphoid cells, since decreased cytotoxic activity of phagocyte-contaminated crude lymphocyte fraction was recovered by elimination of contaminating phagocytes. The monocytes inhibited ADCC of lymphoid cells through phagocytosis of SRBC. This assay system may be useful for estimating not only Fc receptor-mediated cytotoxicity but also Fc receptor-mediated phagocytic activity of human peripheral blood leukocytes.View full abstractDownload PDF (1253K) -
Takashi Fujibayashi, Osamu Sato, Yuzo Takahashi, Hideo ItohArticle type: Article
1979 Volume 28 Issue 7 Pages 568-577
Published: July 30, 1979
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSWe propose a method for computing the cytotoxic activity of T cells(CTA_T)and non-T cells(CTA_<non-T>)in cell-mediated cytotoxicity tests. Microcytotoxicity assays against a cultured cell line derived from oral carcinoma were performed with peripheral blood lymphocytes from 10 healthy donors and 40 oral cancer patients. The effector cells were divided into unfractionated(Unf), macrophage-depleted(Mφ-dep), E_N-rosette forming(Ep), and E_N-rosett non-forming(Ei)fraction and subpopulations and % cytotoxicity were assayed. Simultaneous equations were made by regarding CTA_T and CTA_<non-T> as two unknowns of the equations composed of T cell %, non-T cell % and % cytotoxicity of both Mφ-dep and Ep. The % cytotoxicity calculated by substituting computed CTA_T and CTA_<non-T> values into the equation consisting of measured Unf values proved to be very close to the experimental % cytotoxicity values. Therefore, the validity of the above computation from the simultaneous equations may be partially justified by this evidence. The mean value of CTA_T From cancer patients was significantly higher than that of health donors. As for the relationship with the clinical stage, the early stage showed significantly higher mean values of CTA_T than the advanced stage. On the other hand, no significant difference regarding CTA_<non-T> was observer. Therefore, T cell mediated cytotoxicity showed disease- and stage-related reactivity, whereas non-T cell mediated cytotoxicity was considered to be disease-non-related reactivity, suggesting natural killer activity.View full abstractDownload PDF (1181K) -
Takashi Horie, Tsuneto Akashiba, Satoshi Enomoto, Michiya Yamaguchi, K ...Article type: Article
1979 Volume 28 Issue 7 Pages 578-585
Published: July 30, 1979
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSWe examined the site of airway obstruction in EIA. Pulmonary function tests and flow volume curves with air and HeO_2 were performed with 4 EIA patients and 4 normal subjects before and after exercise on a bicycle ergometer. In normal subjects, exercise resulted in no remarkable changes in pulmonary function data, while in EIA patients, reduction in PF, V_<50>, V_<25> and MMF, and elevation of Raw were observed. 〓V_<50>(V_<50HeO2>/V_<50air>) was above 120% in all EIA cases before suggesting central airway obstruction, however, it was reduced significantly in 3 cases after exercise suggesting peripheral airway obstruction. There 3 cases showed higher CV and Visov, and lower V_<25> and MMF before exercise indicating the existence of peripheral airway obstruction. Based on 〓V_<50>, it has been reported that the predominant obstruction is in central airway and its location does not change before and after exercise in EIA. Our results indicated that higher 〓V_<50> suggesting central airway obstruction was frequently accompanied with lower V_<25> and MMF, and higher Visov indicating peripheral airway obstruction. Therefore several flow parameters should be considered to examine the site of airway obstruction in addition to 〓V_<50>.View full abstractDownload PDF (884K) -
Takayuki Sugiura, Yasuo Masuzawa, Toshiaki OsawaArticle type: Article
1979 Volume 28 Issue 7 Pages 586-594
Published: July 30, 1979
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESS1. Several organic and inorganic mercury compounds were examined for their ability to stimulate human peripheral lymphocytes. We found that divalent mercury compounds are mitogenic to human peripheral lymphocytes, while monovalent mercury compounds are not. A mercury-dextran derivative(MW250000)(7.5-15Hg atoms on one molecule)acting only at the outer surface of the plasma membrane failed to stimulate lymphocytes. However, the similar mercury-dextran derivative (MW70000)which can penetrate into the plasma membrane was mitogenic to lymphocytes. Maximum stimulation of lymphocytes induced by mercury compounds was noted on the 6th day. 2. T- and B-lymphocytes were purified by E-rosette and nylon wool column techniques. Mercuric chloride stimulated only T-lymphocytes. The participation of monocytes was not required for this activation of T-lymphocytes. 3. Mercuric chloride accelerated the incorporation of ^3H-thymidine by T-cell line MOLT-4F lymphoblastoid cells. The optimal concentration of mercuric chloride was equal to that for stimulation of human peripheral lymphocytes. Mercuric chloride did not accelerate the incorporation of ^3H-thimidine by B-cell line lymphoblastoid cells. These results suggest that the mechanism of T-cell activation with mercury compounds is different from that with plant lectins.View full abstractDownload PDF (897K) -
Hideo BannoArticle type: Article
1979 Volume 28 Issue 7 Pages 595-601
Published: July 30, 1979
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESS1. The cGMP in nasal mucosa of non-treated guinea pigs was 14.4±6.7pmol/g, and the cAMP was 770±179pmol/g (N=10). 2. In sensitized guinea pigs compared with non-treated group, the cGMP increased in nasal mucosa, decreased in plasma, but showed no changes in trachea and lung. On the other hand, the cAMP increased in nasal mucosa, trachea and plasma. 3. In attack-induced group by re-inhalation of konjac "Maiko", compared with sensitized group, the cGMP of trachea increased form 29.0±8.6pmol/g(N=10) to 40.9±13.7pmol/g (N=10)(p<0.05), and remarkably increased in lung and plasma(p<0.01). The cAMP of lung also remarkably increased from 2440±303pmol/g(N=10) to 3913±885pmol/g(n=10)(p<0.01), and slightly increased in plasma(p<0.1). The ratio of cGMP: cAMP increased in lung(0.260±0.130→0.468±0.233)(p<0.05) and plasma(0.222±0.067→0.468±0.249)(p<0.01). 4. In the levels of cGMP and cAMP, a statistically significant relationship was found between plasma and nasal mucosa. Between plasma and lung, a statistically significant relationship was also found in the levels of cGMP and the ratio of cGMP: cAMP. On the other hand, between plasma and trachea, a statistically significant relationship was not found in the levels of cGMP, cAMP and the ratio of cGMP: cAMP, however these cyclic nucleotides changed together in the same direction. Therefore, the changes in the levels of cyclic nucleotides in airway tissue may be estimated by measurement of the levels of plasma cyclic nucleotides.View full abstractDownload PDF (867K) -
Tokuko MukoyamaArticle type: Article
1979 Volume 28 Issue 7 Pages 602-606
Published: July 30, 1979
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSLeukocyte adherence inhibition (LAI) assay was previously shown to be an in vitro correlate of delayed type hypersensitivity in animals. In order to test its applicability to man, especially children, the tests were performed using peripheral blood leukocytes from 20 healthy and 23 asthmatic children. Leukocytes from all 11 children with positive tuberculin skin reaction showed significant adherence inhibition in the presence of PPD, while cells from 9 individuals with negative skin reaction did not. Furthermore, children with strong skin reactivity tended to have a high % inhibition of LAI. Data on two other antigen system, namely Candida albicans and house dust, indicated a similar correlation between delayed type skin reactions and LAI. Leukocytes from children with positive skin reactions to an antigen showed positive LAI to the specific antigen. These results indicate that the LAI may be useful in vitro assay for cell-mediated immunity, since the assay requires less time and material than most of the conventional in vitro assays.View full abstractDownload PDF (552K) -
Article type: Appendix
1979 Volume 28 Issue 7 Pages 607-
Published: July 30, 1979
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (283K) -
Article type: Cover
1979 Volume 28 Issue 7 Pages Cover30-
Published: July 30, 1979
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (148K)
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