All issues
Volume 32, Issue 9
Displaying 1-15 of 15 articles from this issue
- |<
- <
- 1
- >
- >|
-
Article type: Cover
1983 Volume 32 Issue 9 Pages Cover33-
Published: September 30, 1983
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (43K) -
Article type: Cover
1983 Volume 32 Issue 9 Pages Cover34-
Published: September 30, 1983
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (43K) -
Article type: Bibliography
1983 Volume 32 Issue 9 Pages Misc11-
Published: September 30, 1983
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (373K) -
Seiichiro Namai, Ryosuke Ikemori, Sohei MakinoArticle type: Article
1983 Volume 32 Issue 9 Pages 929-939
Published: September 30, 1983
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSHypersensitivity reactions following sting by Hymenoptera are induced by IgE-mediated anaphylactic reactions. Using venom antigens of wasps, yellow jackets and honeybees, intracutaneous tests, scratch tests and RAST were carried out on 14 normal subjects and 15 patients who had had episodes of generalized anaphy1actic reactions to stinging by Hypenoptera. The aims of this study were to determine the optimal concentrations of antigens to be used in skin-testing to diagnose the hypersensitivity, to eva1uate RAST measurement in the diagnose of hypersensitivity, and to investigate the cross-reactivity of 3 venom antigens. 1) On the scratch test, when a concentration of 1000μg/ml of the antigens were used, all the nomal subjects showed a negative response, while all except 3 of the hypersensitive subjects showed positive reaction 2) On the intracutaneous test, when 1.0μg/m1 of the venom antigens were used, all of the normal subjects showed a negative response, while most of the hypersensitive subjects (100% in the case of wasp venom and 93.3% in yellow jacket) showed positive response. On the other hand, positive responses to honeybee venom antigens were found in 40% of the hypersensitive subjects. 3) Sixty and 73.3% respectively, of the hypersensitive subjects showed a positive response on RAST to wasp and yellow jacket venom antigens, while only 13% of the hypersensitive subjects showed positive response to honeybee venom antigen. 4) End points of intracutaneous tests with wasp and yellow jacket venom antigens showed significant correlation, suggesting cross-reactivity of these 2 antigens. However, there was no significant correlation either between the response to wasp and honeybee venom antigens, nor between the response to yellow jacket and honeybee venom antigens.View full abstractDownload PDF (1208K) -
Yoshio Sakamaoto, Takemasa Nakagawa, Koji Ito, Terumasa MiyamotoArticle type: Article
1983 Volume 32 Issue 9 Pages 940-946
Published: September 30, 1983
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSA new method for measurement of IgG antibodies was developed by using antigen-coated polystylene tubes and ^<125>I-anti-IgG-Fc. The sera from 65 asthmatic patients with positive skin reaction against house dust mite, Dermatophagoides farinae (D.farinae), were studied for the measurement of IgG antibodies specific to D.farinae by IgG-RAST. The results of IgG-RAST were compared with double antibody antigen-binding assay and protein A solid-phase radioimmunoassay. There was a good correlation between IgG-RAST and double antibody antigen-binding assay (r=0.90, p<O.001), and to protein A solid-phase radioimmunoassay (r=0.88, p<O.001). Thirty-seven out of the 41 patients receiving immunotherapy with D.farinae, or house dust extract had IgG antibodies exceeding the normal upper limit (6.8μg/ml). In about ha1f of the patients not receiving immunotherapy, the amount of IgG antibodies exceeded the normal upper limit. Eight patients were evaluated for IgG antibody amount before and after immunotherapy. In five of them IgG antibody amount after immunotherapy increased approximately two to threefold above the amount before immunotherapy.View full abstractDownload PDF (718K) -
Kiminobu MinagawaArticle type: Article
1983 Volume 32 Issue 9 Pages 947-959
Published: September 30, 1983
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSCell desensitization can be achieved in the presence of calcium, using subthreshold, suboptimal or supraoptimal concentrations of antigen. It should be noted that, in order to induce desensitization with suboptimal concentration of antigen, basophils will be preincubated with their specific antigen or anti-IgE at a concentration which gives 5 to l5% histamine release when the desensitizing antigen is given in a single step. When higher concentrations of antigen were used, paradoxical results were obtained, in that human basophils triggered by an optimal concentration of antigen after having been exposed to the same kind of desensitizing antigen in the presence of calcium released less histamine than those triggered by buffer after desensitization under the same conditions. It might be suggested that the antigen as a trigger made the cell membrane more stable against the mechanical or physical stimulation by manipulation of the cells for the experiment, thus halting the subsequent release of histamine. Incidentally, EDTA was not added to stop histamine release when all the experimentation was already finished. On the basis of this phenomenon, it might be suggested that the stabilization of the cell membrane against histamine release is a natural part of the cell desensitization process. At low temperature of 4℃, the desensitization rate decreased significantly and the cell desensitization could be induced under Ca^<2+>-free conditions. The experimental data which were obtained in this study might support, though do not prove, the possibility that desensitization may result from increased membrane stability due to increased membrane fiuidity.View full abstractDownload PDF (1567K) -
Kazuhiko WatanabeArticle type: Article
1983 Volume 32 Issue 9 Pages 960-969
Published: September 30, 1983
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSOne hundred and fifty two asthmatic children were orally administered aminophylline (AP) and the influence on the immediate intradermal sensitivity was demonstrated by house dust antigen. They were divided into two groups, one group receiving a single dose of AP 4 to 5 mg/kg of weight and the other 4 days' round-the-clock (RTC) doses of AP 20 to 25 mg/kg/day. The average diameter of wheals and flares were compared between these groups before medication and 3, 6 and 12 hours after medication. The correlation with serum theophylline (TP) was also studied. The average diameter of cutaneous reaction was described as percent suppression:(average diameter before drugaverage diameter after drug)/(average diameter before drug)×100%. Percent suppression was not significant among the single dose group, but significant among the RTC group on wheal and flare reaction at 3 hours(p<0.01). The suppressive effect was evident at the serum level of TP of 6μg/ml and significant at 9μg/m1 (p<0.001). The correlation coefficient between serum TP levels and percent suppression of wheal size was r=0.60 and of flare was r=0.67. In conclusion, it appears advisab1e to avoid theophylline therapy for more than 12 hours before immediate skin testing.View full abstractDownload PDF (1120K) -
Shiro Seto, Takashi Miyake, Takao HiraoArticle type: Article
1983 Volume 32 Issue 9 Pages 970-978
Published: September 30, 1983
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSPeripheral blood lymphocytes from 21 patients with MCLS, in both acute and convalescent phases, were found to be characterized by reactivity with monoclonal antibodies (OKT series) to the surface anitigens of helper-inducer (OKT4) and suppressor-cytotoxic (OKT8) T cell subsets and to a common T cell antigen (0KT3). We made the following observations: 1) In acute phase (5-10 days of illness), patients with MCLS had a significantly reduced percentage of OKT-3 positive (T3+) cells (p<0.001), OKT4-positive (T4+) cells (p<0.01) and OKT8-positive (T8+) cells (p<0.001) in contrast to each percentage in convalescent phase. Meam±SD was 52±4%, 40±3% and 12±3%, respectively. 2) In convalescent phase (26-37 days of illness), the percentage of every subset was increased. T3+was 60±5%, T4+was 43±4% and T8+ was 18±3%. 3) The absolute number of T3+(p<0.05) and T8+(p<0.001), but not of T4+, was a1so significantly reduced in acute phase of MCLS. 4) The ratio of T4+ to T8+ cells was increased in 19 of 21 patients in acute phase. Its tatio of 3.4±0.9 was significantly different from 2.4±0.6 in convalescence (p<O.001). Thus, abnormalities of immunoregulatory T cell subsets were observed in acute phase of MCLS.View full abstractDownload PDF (979K) -
Yasuhiro Mizoguchi, Hiroko Tsutsui, Takeyuki Monna, Yamamoto Sukeo, Se ...Article type: Article
1983 Volume 32 Issue 9 Pages 979-984
Published: September 30, 1983
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSAnti-LSP antibody-coated hepatocytes were damage by incubation with peripheral blood mononuclear cells. This antibody-dependent cell-mediated cytotoxicity (ADCC) was demonstrated by estimation of the reduction of protein synthesis in target cells. Such liver cell damage was also found in experiments in which culture supernatant separated from ADCC reaction mixture was added to the isolated liver cells. In addition, the active substances were fractionated as definitive materials by gel filtration. Addition of the culture supernatant to freshly prepared hepatocyte suspension induced, in addition to in hibition of protein synthesis, significant enhancement of lipid peroxide formation in the liver cells. These results suggested to us that lipid peroxidation may play an important role in the induction of ADCC-mediated damage to liver cells.View full abstractDownload PDF (535K) -
Yukiko ItoArticle type: Article
1983 Volume 32 Issue 9 Pages 985-995
Published: September 30, 1983
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSIgG and IgE antibodies in serum and nasal secretions were studied in 31 patients with allergic rhinitis induced by Japanese cedar pollen, 7 healthy forest workers and 7 other normal controls;immunotherapy was begun for 23 of the patients and the other patients were untreated. IgG antibody was measured by the radioimmunoassay technique, and IgE antibody was measured by RAST. Serum IgG antibody of the patients in immunotherapy was significantly higher than that of the untreated patients, and that of the latter was significantly higher than that of the normal controls. There was no significant difference in serum IgG antibody levels between the untreated patients and the forest workers. Serum IgG antibody levels increased significantly during the preseasonal immunotherapy. However, IgG/albumin ratio in nasal secretions showed no rising tendency. In the forest workers who have been receiving severe seasonal exposure to cedar pollen, serum IgG antibody levels and IgG/albumin ratio in nasal secretion appeared to increase during the pollen season. Serum IgE antibody levels rose significantly in both the patients in immunotherapy and the nutreated patients during the pollen season. During the first few months of immunotherapy, IgE antibody in serum and nasal secretions has not been demonstrated to increase over the pretreatment titers. These results suggest the possibility of local production of IgG antibody in response to natural exposure to cedar pollen. The effect of immunotherapy on IgG antibody levels in serum and nasal secretions is discussed.View full abstractDownload PDF (1166K) -
Norihisa Ishii, Ryukichi Nagai, Kenji OkudaArticle type: Article
1983 Volume 32 Issue 9 Pages 996-998
Published: September 30, 1983
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSTo determine the rules goveruing the selection of class II molecules (A versus E)in the process of recognition of different antigens. We tested T cell proliferative response to GA, GLT, and LDH_8. Strains that responded were then tested in the antibody-blocking assay to determine the class II context of the response. The response to GA and LDH_B always occurred in the context of the A molecule. In two instances of LDH_8, the E molecule maybe provided the context for the stimulation of suppressor T cells. The response to GLT occurred in the context of the E molecule except in the case of two strains. These data indicate a remarkable but puzzling consistency in the channelling of the response to a given antigen via either A or E molecules. This consistency may be a hint that there is a link between the specificity of antigen (nonself-) and Mhc (self-) recognition by T lymphocytes.View full abstractDownload PDF (367K) -
Article type: Appendix
1983 Volume 32 Issue 9 Pages 999-
Published: September 30, 1983
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (36K) -
Article type: Appendix
1983 Volume 32 Issue 9 Pages 1000-1003
Published: September 30, 1983
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (272K) -
Article type: Bibliography
1983 Volume 32 Issue 9 Pages Misc12-
Published: September 30, 1983
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (9K) -
Article type: Cover
1983 Volume 32 Issue 9 Pages Cover35-
Published: September 30, 1983
Released on J-STAGE: February 10, 2017
JOURNAL FREE ACCESSDownload PDF (219K)
- |<
- <
- 1
- >
- >|