日本ウズラ(CoturnixcoturnixjaPonica)から,従来の記載にない1種類のEimeriaを純粋分離し,その形態,生物学的諸性状,宿主特異性について観察を行なった.オーシストには,ミクロパイルは認められず,大きさは平均18.4×14.1μ,外部残体は認められなかった.Polarinclusionはほとんどのものにみられた.胞子形成時間は,25。Cで22~24時間.Prepatcntpcriodは6日,Patcntperiodは約11日間であった.寄生部位は盲腸のみである.肉眼的には,盲腸粘膜の肥厚と点状出血,組織学的には,第2代シゾントはおもに粘膜組織内にみられ,あるものは粘膜筋板,および輪走筋に接した所にまでみられた.またこれらの成熟したものは,PAS反応陽性であった.しかし,スポロゾイト,初代シゾントおよびガメートサイトは,盲腸の絨毛上皮細胞中に寄生していた.また,このEimeriaは日本ウズラと近縁の鳥類である二ホンキジ・コウライキジ・ヤマドリ・コリンウズラ・コジュケー・ニワトリなどには感染が成立せず,一方,これらの鳥類から分離したEimeriaは,いずれも日本ウズラに感染しなかった.以上の結果から,今回分離したEimeriaは,日本ウズラを宿主とする新種と認め, Eimeγiatsunodaiと命名した.

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ヒトにおいて多数例報告されている大動脈弓とその分枝異常は,ウシ,ブタ,イヌ,ネコなどの家畜においても観察されている.家畜において,これらの分枝異常が発見された背景を文献的に考察してみると,先天的な右側大動脈弓遺残による食道狭窄などが主な原因となって,顕著な臨床症状を伴っている場合が極めて多い.したがって臨床症状を伴わないこれらの分枝異常に関する報告は甚だ少ない.特に臨床症状を伴わないイヌの大動脈弓分枝異常についての報告は,遠位の右鎖骨下動脈を最終技とする分校異常に関するものである.今回,著者らが観察したと同様な分枝異常についての報告は見当たらない.著者らは,160例の大体を解剖し,そのうち,雌の雑種成犬2例に,極めて類似した右鎖骨下動脈破格を認めた.これらの状態を観察するとともに,発生学的に若干の検討を加えてみた.それらを要約すると,次の通りである.1.正常位に位置する大動脈弓は,腕頭動脈に相当する総頚動脈幹,左鎖骨下動脈,右鎖骨下動脈の3枝を分枝し,それらの起始部の位置は極めて近接していた.2.総頚動脈幹の先端は2枝のみに分岐し,左・右総頚動脈となる.本来,腕頭動脈から分岐すべき右鎖骨下動脈は,左鎖骨下動脈起始部の右側から,大動脈弓より直接単独に分岐し,食道と気管の背側を右側に向かって,斜め頭方に通過していた.3.右鎖骨下動脈による食道の狭窄は認められなかた.

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The purpose of this study was to make clear tlxe morphological changes in the skullsize, paritcularly the post-natal dexzelopment of the skull, of the Yezo brown bear (Urususarctos yezoensis lyd.) . The characteristics of development were classified into the growthof sizes, obliteration of suturae, and abrasion of teeth.All t]te skulls were measured by the method employed by SAITO (1963). As acriterion to tell the sex of a skull could be used the shape of temporal ridges and sagittalcrest of animals, or more of age, as was the case with American black bears observed byMARKS and ERICI<SON (1966). It was difficult, however, to differentiate the sex bythe shape of canine teeth. The results of measurement were analyzed statistically. The width of the craniunx(Eu-Eu) attained a maximum both in males and in females till twelve montlns of age. Itwas about 95mm. In the adult skulls, the mean value of the basilar length (B -P) was327tnm in males and 277mm in females. The sex difference was found to be very largein many items of the relative measurements, but the rate of mandibular length to basilarlength (Id -Cm/B -P) was comparatively constan?t. Its value was abottt 0.75. In theregressiwe liraes, oualy the rate of total length to basilar length (Op -P/B -P) showed posi-tixe growth. All the other rate showed negative growth.The skull of the adult bear contained the very large sinus [ror?talis. The morpho-oezaesis of the cranial sinuses was discussed to some extent.

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臨床的に正常な5頭の緬羊の末梢血から,溶血法によって収集した好塩基球の微細構造を,電子顕微鏡を用いて観察した.その所見を要約すると,次のとおりである.1)好塩基球の核は,1個あるいは数個に分葉しており,核の内部は,明瞭な斑状構造を示していた.2)好塩基球の特殊顆粒は, unitmcmbrancに包まれ,円形ないし楕円形で,その大きさは0.4~1.0(平均0.67)μであった.これらの顆粒は,dcnsityおよび内部構造を基礎にして,次の3型に分類された.すなわち,A型は均質でdcnsityの高い顆粒,B型は,粗大顆粒状の網状構造を有するdcnsityが高い顆粒,C型は,微細顆粒状の網状構造を有するdensityが低い顆粒である.A型顆粒は,BおよびC型のそれよりも,出現数は少なかった.3)細胞質内には,少数のミトコンドリ乙 ゴルジー装置,多数の小胞,少量の粗面小胞体,およびmultivesicularbodyが観察された.

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豚における肝細胞癌の2例について,電顕的検索を行なった.種々の変化が,癌細胞の核,ミトコンドリ乙小胞体,および病巣内における胆細管に見られた.肝小葉の構造は,癌病巣内には認められなかった.静脈洞が時には癌巣内に観察ざれた.静脈洞内には,膠原線維の侵入および種々の骨髄細胞が認められた.

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In purifying the transaminase in fowl blood plasma, it is necessary to use a methodfor systematic separation of the blood plasma. No detailed investigations on this aspecthave been reported. Then, during the days previous to the subfractionation of plasmaprotein components, the plasma was separated by means of successive fractional precipi-tation at a controlled pH value. The isoelectric point of transaminase in the fowl bloodplasma was determined as follows.In addition, the aspartate aminotransferase GOT) and alanine aminotransferase(GPT) reported in this paper could be classified into four types known by the generalterms F-GOT, R-GOT, F-GPT, and R-GPT. These terms were used as synonyms offorward-GOT, reverse-GOT, forward-GPT, and reverse-GPT, respectively. Each of themwas divided by the kind of transaminase that was directly related to the reversible trans-amination reaction.(a) F-GOT: pH 7.2 and 4.9(b) R-GOT: pH 6.9 and 5.2(c) F-GPT: pH 5.8 and 4.6(d) R-GPT : pH 6.9 and 4.6These findings offered helpful suggestions for the fractionation of transaminases bythe use of Cohn and Oncleys methods. Then, of these methods, the 6th and 9th metI?odswere used experimentally for the purpose of fractionating the transaminase. As a result, it was found that the 9th method of ONCLEY was effective for practical purposes. Is-pecially, the fractions of IV-5, 6, 7, and 8 that had been subfractionated from Cohnsfractions.IV-4 by the 9th method of ONCLEY showed activities higlter than the remaining fractions, In those fractions, especially that of IV-5, nothing could comperasate the appearanceof F-GOT and F-CPT activities, but only R-GOT and R-GPT activated the fraction ofIV-5. This result suggests that there may be some correlation concerning the mode offractional precipitation between R-GOT and R-GPT. More detailed information 011this aspect is to be given in a report to come.In this way, each of the active fractions for individual transaminase is no good agree-ment concerning the mode of fractional precipitation.On the basis

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The present animal experiment was designed to clarify or otherwise elucidate themetabolic response, especially that of glucuronidation, in animals administered withmassive doses of glucosidurogenic drugs.In his review of literature, DUTTON (1966) mentioned that a specific stimulation ofUDP-glucuronyltransferase (GT) had not been achieved by treatment with an acceptorsubstrate, and that no drug tolerance had been correlated with an increased GT activityfor the drug or its metabolites. HARTIALA et al. (1966) reported on the competitiveinhibition of GT by eugenol in vitro.The present paper deals with this inhibition by eugenol. The influence of eugenolon the glucuronidation in rats by oral administration was also observed.The following results were obtained.l) A 50% inhibition was shown by 1.1 X 10 M of eugenol upon substrate (2.0X10 M of p-nitrophenol)-enzyme (5, ODD X g, 10 min. supernatant of rat liver homogenate)system (Fig. 1).2) The nature of inhibition was competitive. The Ki value was 0.5xI0-4 M. Inaddutuon, the Km value was 1.7 X 10 M in this experiment (Fig. 2).3) When a large amount (200 mg, about a half of LD..) was administered orallyto adult male rats, a large amourit of the ether type of glucuronide (0-G) was excretedinto the urine (Table l), and a significant increase was observed in GT activity of theliver (Table 2).From these results, it was concluded that eucenol was one of the most electivebglucosidurogenic drugs, and that liver GT might be induced in the rat by oral administra-tion of eugenol.

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