日本獸醫學雜誌(The Japanese Journal of Veterinary Science)
Online ISSN : 1881-1442
Print ISSN : 0021-5295
ISSN-L : 0021-5295
37 巻, 5 号
選択された号の論文の11件中1~11を表示しています
  • 小西 辰雄, 一条 茂, 小川 寿美雄
    1975 年 37 巻 5 号 p. 227-238
    発行日: 1975/10/25
    公開日: 2008/02/13
    ジャーナル フリー
    Clinical and clinico-pathological observations were made on three cattle affected with generalized amyloidosis. The results obtained are as follows. 1. The main clinical symptoms of the three cattle were anorexia, continued watery diarrhea, edema of the subcutaneous tissue, and rapid emaciation. The clinical duration was 13 to 34 days. Prognosis was bad without any therapeutic effect in all the cattle. 2. The characteristic biochemical findings were advanced hypoproteinemia accompanied by hypoalbuminemia, hypobetaglobulinemia, hypogammaglobulinemia and hyperalphaglobulinemia. In the immunoelectrophoretic pattern of serum, precipitin lines were strong for alpha 1 lipoprotein and alpha 2 macroglobulin and mild for albumin and IgG. 3. Advanced renal proteinuria was shown by the urine which contained such protein components as albumin, alpha-, beta- and gammaglobulin. In the urinary sediment, there were renal epithelial cells, hyaline casts and leukocytes in all cases. 4. Severe amyloidosis was observed in all the affected cattle. Amyloid deposits were found in the wall of small blood vessels in almost all the organs, including the liver, spleen, pancreas, adrenal cortex, lymph nodes, alimentary tract and thyroid gland. The alimentary tract showed serious edematous changes in the submucosal tissue. In addition, there were multiple abscesses in the lungs (Case No.1), chronic mastitis and suppurative peritonitis (Case No.2) and chronic mastitis (Case No.3). 5. No incidental causes of generalized amyloidosis were unknown in any case. It was suggested that the disease might follow the chronic infections described above.
  • 後藤 仁
    1975 年 37 巻 5 号 p. 239-245
    発行日: 1975/10/25
    公開日: 2008/02/13
    ジャーナル フリー
    The Obihiro strain of feline panleukopenia (FPL) virus agglutinated swine erythrocytes whichi had been examined in advance for sensitivity to hemagglutination (HA) by the virus. All the reagents for the HA test were made of 0.01 M phosphate-buffered saline solution adjusted to a pH of 6.8. Specific HA reaction was recognized after a 2 hours' incubation period in a refrigerator at 4°C. It was inhibited by specific immune serum to the virus in the hemagglutination-inhibition (HI) test. There was a certain correlation between HI and neutralizing antibody titers by almost all the feline serum somples tested. Viral hemagglutinin was shown to be stable to ether and heat. In the virus fluids HA titers were not lost after sonic treatment of the virus, but were completely lost after ultraviolet irradiation. The results are in almost complete agreement with the physicochemical properties of the infectivity of the virus in cat kidney cell cultures. Treatment of the virus with potassium periodate solution had essentially no effect on the adsorption or the elution of the virus with swine erythrocytes. The virus, however, failed to agglutinate erythrocytes pretreated with a valid concentration of receptor-destroying enzyme. On the basis of these findings, the significance of the parvovirus-erythrocyte interaction was discussed.
  • 小西 信一郎, 望月 雅美, 尾形 学
    1975 年 37 巻 5 号 p. 247-257
    発行日: 1975/10/25
    公開日: 2008/02/13
    ジャーナル フリー
    Seven strains of feline panleukopenia (FPL) virus were isolated in feline kidney cell cultures from 2 of 4 spontaneous cases of FPL, and in direct cultures of the kidney obtained from 2 of 3 FPL cases. All of them exhibited cytopathic changes accompanied by formation of intranuclear inclusion bodies in feline kidney cell cultures, and hemagglutinated porcine erythrocytes at 4°C. They could not be distinguished from one another by serum-neutralization (SN) or hemagglutination-inhibition (HI) tests. Discussion was made on biological and physicochemical properties, the routinization of isolation and quantitative determination methods, and the SN and HI tests as serologic procedures for FPL virus.
  • 小西 信一郎, 明石 博臣, 泉対 博, 尾形 学
    1975 年 37 巻 5 号 p. 259-267
    発行日: 1975/10/25
    公開日: 2008/02/13
    ジャーナル フリー
    Two strains, the Bibuna and the Bucyrus, of equine arteritis virus (EAV) were tested for propagation in various cell cultures. They were grown in primary cell cultures of horse, rabbit, hamster, cat and pig kidney, and in established cell cultures of baby hamster kidney (BHK-21/13), hamster lung (HmLu), African green monkey kidney (Vero) and Cynomolgus monkey kidney (JINET). Cytopathic changes were comparatively common to all cells, and characterized by rounding and vacuolation of the cells and by increased density of the cytoplasm. Most affected cells were detached from the glass wall within 7 days after inoculation. The infectivity titers of the primary cell culture fluids were 104 to 106 TCID50/0.2 ml, and those of the established cell culture fluids 106 to 108 TCID50/0.2 ml. Both strains were able to replicate in the presence or absence of 5-iodo-2-deoxyuridine (IUDR). They were not stabilized by cation at 50°C, but were highly sensitive to ether and sodium deoxycholate, and moderately sensitive to heat and acid. The Bibuna strain was more sensitive to a high concentration of trypsin than the Bucyrus strain. Each virus formed distinct piadues 2 to, 3 mm in diameter on the monolayer of Vero cells. Serum samples were collected from 107 horses in Japan in 1972 and 1978 and tested for EAV antibody by neutralization tests in monolayer cell culture tubes or plaque reduction neutralization tests in Vero cells. No antibody, however, was detected from any sample so far.
  • 前出 吉光
    1975 年 37 巻 5 号 p. 269-272
    発行日: 1975/10/25
    公開日: 2008/02/13
    ジャーナル フリー
    Erythrocyte lifespan was studied in cats infected with Haemobartonella felis, using 51Cr as an isotopic label. Only 5 to 10 per cent of 51Cr-labeled erythrocytes were parasitized with H. felis, though their osmotic fragility increased markedly. For the first 3 days after transfusion of 51Cr-labeled erythrocytes, the amount of labeled erythrocytes destroyed in infected cats was approximately twice that in non-infected cats, while no parasites appeared on any erythrocyte for that period. The average half-life of erythrocytes was 4.3 days in infected cats and 8.8 days in non-infected cats. From the results obtained, it was concluded that erythrocytes were affected so severely with H. felis in infected cats even when the parasite was released from them that their lifespan was impaired.
  • 平棟 孝志, 村瀬 信雄, 梁川 良
    1975 年 37 巻 5 号 p. 273-276
    発行日: 1975/10/25
    公開日: 2008/02/13
    ジャーナル フリー
    The efficacy of antibiotic treatment in Corynebacterium renale infection was examined employing 26 cows whose serum antibody to C. renale was negative and thus diagnosed as to be suffered from cystitis alone and 19 cows whose serum antibody was positive and diagnosed as to be affected with pyelonephritis. The therapy was more successful in cows affected with cystitis alone than those affected with pyelonephritis. With regard to cows affected with cystitis alone the treatment was successful in the case of infection with C. renale of types I and II, but was unsuccessful in the case of infection with type III organisms.
  • 吉川 尭, 小山田 隆
    1975 年 37 巻 5 号 p. 277-287
    発行日: 1975/10/25
    公開日: 2008/02/13
    ジャーナル フリー
    Fifty-three spontaneous cases of papillary tumor in the bovine urinary bladder encountered in Towada, Aomori Prefecture, were studied histopathologically. The animals involved occupied 3.1% of the cattle kept in this city. A high incidence was shown in bovine strains known for the frequent occurrence of haematuria vesicalis bovis. Macroscopically, localized cancerous growths were found, ranging from small granule to child-fist in size. Property and development of these changes varied from one individual to another. Histopathologically, all the lesions showed papillomatous or papillomatous-cancerous growths derived from the transitional epithelium. They were called papillary carcinoma collectively and classified into types I, II and III on the basis of the degree of malignancy. In 10 cases, weakly basophilic intranuclear inclusion bodies were present in the tumorous epithelial cells. The morphogenesis of these lesions was briefly discussed.
  • 鈴木 真理, 野村 晋一
    1975 年 37 巻 5 号 p. 289-293
    発行日: 1975/10/25
    公開日: 2008/02/13
    ジャーナル フリー
    Electromyographic studies on the deglutition movement were carried out with ten adult fowls. In the unanesthetized fowls, bipolar electrodes of 120μ enamel-covered copper wire were inserted into 13 muscles of the throat and the esophagus. The deglutition reflex was elicited by tactile stimulation of the tip of the tongue with a wet cotton swab. In the fowl, the deglutition movements were rapid rostro-caudal tongue movements. Tonic activities were seen in the m. hyoglossus and the m. styloentoglossus during the deglutition. These muscles concaved the hyoid apparatus ventrally. The m. stylomandibularis and the m. laryngohyoideus acted synchronously in such manner as to pull the hyoid apparatus backward in each movement. The m. articulohyoideus dorsalis and ventralis and the m. transversus hyoideus acted to restore the hyoid apparatus after the activities of the m. stylomandibularis and the m. laryngohyoideus. From the results, the muscles connecting the mandible or the larynx with the hyoid apparatus and those adhering to this apparatus were known to play an essential role to move the hyoid apparatus with the tongue at the time of deglutition in fowls.
  • 高橋 清志, 山下 正亮, 清水 悠紀臣
    1975 年 37 巻 5 号 p. 295-301
    発行日: 1975/10/25
    公開日: 2008/02/13
    ジャーナル フリー
    1. Theileria sergenti 実験感染牛では, 抗体価がピークに達した後, 寄生率は低下し, 赤血球容積が増加した. また, 急性貧血で死亡した摘牌牛では, 抗体を検出できなかった. 2. 自然感染発病牛は発病時においても高い抗体価を有し, 回復期にはさらに上昇する傾向があった. 3. 自然感染および実験感染から, 臨床的に回復した牛に対して, 再攻撃した結果, 寄生率の上昇や貧血は認められなかった. 4. 感染耐過牛に副腎皮質ホルモンを投与した結果, 抗体価は変動しなかったが, 赤血球内寄生率は急激に上昇して発症した. 以上の結果から, T. sergenti の免疫機構に, 液性抗体が一定の役割を演じていることは明らかである. しかし再発例にみられたように, 細胞性免疫の関与も無視できない. 本論文の要旨は, 第77回日本獣医学会において報告した.
  • 五藤 精知, 板倉 智敏
    1975 年 37 巻 5 号 p. 303-306
    発行日: 1975/10/25
    公開日: 2008/02/13
    ジャーナル フリー
  • 鈴木 芳和, 岡田 文弘, 干葉 胤孝
    1975 年 37 巻 5 号 p. 307-309
    発行日: 1975/10/25
    公開日: 2008/02/13
    ジャーナル フリー
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