巻号一覧
36 巻 (1974)
- 6 号 p. 459-
- 5 号 p. 363-
- 4 号 p. 285-
- 3 号 p. 195-
- 2 号 p. 73-
- 1 号 p. 1-
36 巻, 5 号
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小島 義夫, 木下 善満, 福田 道雄1974 年 36 巻 5 号 p. 363-374
発行日: 1974/10/25
公開日: 2008/02/13
ジャーナル フリーA Hampshire boar produced an unique form of abnormal spermatozoa (Kojima: 1973), and the boar's testes produced multiple-headed sperm were post mortem investigated histologically. The morphological alterations of the testes were generally hyperplasia of the interstitial tissue and hypoplasia of the seminiferous tubules. The degenerative changes consisted of various kinds of atrophy in the germ cells and disintegration of spermatozoa in the lumen of the seminiferous tubules. With frequent appearance of multinucleated giant cells, there were obviously noticed multiple-headed spermatozoa and also late spermatids in the seminiferous tubules. It is interesting that the multiple headed-spermatozoa were already in existence in the testes. A case of double headed-spermatozoa was also noticed in the interstitial space.抄録全体を表示PDF形式でダウンロード (2604K) -
平井 克哉, 張 靖男, 島倉 省吾1974 年 36 巻 5 号 p. 375-380
発行日: 1974/10/25
公開日: 2008/02/13
ジャーナル フリーA serological survey was carried out to detect hemagglutination-inhibition (HI) antibody against hemagglutinating encephalomyelitis virus (HEV) from the serum of pig. A total of 6, 797 porcine serum samples were collected from 45 prefectures in Japan. As a result, 3, 393 samples (49.9%) had the antibody. Of them, 1, 992 samples (58.7%) had an HI titer of 1:1280 or high. The positive rate varied from 39.6 to 72.0% in each age-group. It was 53.7, 47.0, 39.6, 52.5, 54.4, and 72.0% in the 0-2, 2-4, 4-6, 6-8, 8-l2, and over-12-month groups, respectively. In short, the positive rate became higher in accordance with the advance in age. The occurrence of such a high positive rate as 53.7% in the 0-2 month group, and the same HI titers of suckling piglets as those of their mother sows may be due to the absorption of antibodies from colostrum. The breed of pigs may not be a factor to induce HEV infection. The positive rate was high in the Kinki and Chugoku districts (66.1 and 65.1%, respectively) and low in the Tohoku and Kanto districts (26.6 and 27.8%, respectively). Antibodies against HEV were proved in Japan in 1965 and confirmed in every subsequent year. In 1973, more than 60.0% of the pigs examined were found to possess those antibodies. Therefore, it is concluded that HEV infection may be widespread in Japan.抄録全体を表示PDF形式でダウンロード (658K) -
上田 雄幹, 曲渕 輝夫, 三枝 順三, 浦野 徹, 伊藤 喜久治, 木内 吉寛, 藤原 公策1974 年 36 巻 5 号 p. 381-389
発行日: 1974/10/25
公開日: 2008/02/13
ジャーナル フリーFifteen beagles, 13 males and 2 females, from a breeder colony suspected of Brucella canis (B. canis) infection were autopsied, and isolation of B. canis from various organs and detection of anti-B. canis agglutinin in their sera were attempted. B. canis was isolated from the spleen, lymph nodes, prostate and epididymis at a high frequency and from blood, testis and urinary bladder of some cases. Five of 15 dogs having been treated with tetracycline were shown to harbour smaller number of the organisms than the non-treated ones. Most of the sera showed high agglutinin titres to B. canis, and the presence of incomplete antibody was confirmed in some sera showing only feeble agglutination. Treatment with cortisone or antilymphocytic serum failed to produce recurrent disease in 12 persistently infected males.抄録全体を表示PDF形式でダウンロード (803K) -
輿水 馨, 尾形 学1974 年 36 巻 5 号 p. 391-406
発行日: 1974/10/25
公開日: 2008/02/13
ジャーナル フリーMycoplasmas were isolated from 100 (30.8%) of 324 specimens collected from 81 apparently healthy or affected dogs. They were isolated from nasal cavity (17/23:73.9%), oral cavity (7/7: 100%), trachea (33/46: 71.7%), lung (9/46: 19.5%), liver (1/40:2.5%), kidney (2/38: 5.2%), spleen (2/40: 5.0%), lymph node (4/35: 11.4%), vagina (18/30: 60.0%) and urethra (7/10: 70.0%). Negative results were obtained from 3 specimens of skin and one each of prostate, thyroid, tonsil, testicle, and pleural and peritoneal fluid. A total of 158 strains cloned from these positive cultures could be differentiated into 6 types on the basis of their colonial morphology and various biological and serological characteristics. Of them, 72 strains were identified as M. canis, 28 as M. edwardii, 23 as M. spumans, and 21 as M. maculosum. A sterol-nonrequiring strain isolated from the vagina of a bitch was identified as Acholeplasma laidlawii. Eleven strains isolated from the urethra of male dogs and from the vagina of a female were clearly distinguished from any other previously described canine mycoplasma biologically and serologically, although they were found to be related to one another. In addition, they appeared to have no antigenic relationship to other human and animal mycoplasma species when examined by the growth inhibition test. 0n the basis of the biological and serological characteristics, it is suggested that this group of isolates may belong to a new species of canine mycoplasmas.抄録全体を表示PDF形式でダウンロード (2630K) -
小野 耕一, 土居 俊之, 石川 豊数, 岩 信造, 内藤 又夫, 加藤 四郎, 児山 国明, 高延 壮男, 高久 慶典1974 年 36 巻 5 号 p. 407-420
発行日: 1974/10/25
公開日: 2008/02/13
ジャーナル フリー1970年, 健康なシチメンチョウ105羽中6羽の血液から, アヒル胎児線維芽細胞を用いて, ヘルペス型ウイルスを分離した. これらのウイルスでは, マレック病ウイルスと異なり, 細胞遊離ウイルスを容易に得ることができた. 特にO1株において, その傾向は明瞭であった. 感染培養細胞には, プラークが認められ, プラークは, 多数の多核巨細胞より成っていた. 感染細胞のヘマトキシリン・エオシン染色において, ヘルペス型の核内封入体の形成が認められた. この封入体は, Feulgen 反応およびアクリジン・オレンジ染色により, DNA 陽性を示し, 3H-thymidine のオートラジオグラフィーにおいて, その中にヘルペス型配列の銀粒子が認められた. また DNA 合成阻害剤に対し感受性を示した. さらに感染細胞の超薄切片を電子顕微鏡で観察すると, ヘルペス型ウイルスの構造をもつ粒子が, 核内に多数認められた. これら二重膜粒子の直径は約140mμ, カプシドの直径は, 約100mμであった. しかし, マレック病ウイルス Biken C2 株感染細胞の場合と異なり, カプシドの内部が十字紋様を呈するものが多数認められた. また, 感染材料を燐タングステン酸によりネガティブ染色して, 電子顕微鏡で観察すると, 中空のカプソメア(推定162個)より成るヘルペス型カプシド構造が認められた. これらのウイルスは, 分離当初よリアヒルおよびウズラ胎児線維芽細胞上におけるプラーク形成が明瞭であり, 培養には多核巨細胞も出現した. アヒル, ウズラおよびニワトリ胎児線維芽細胞は, このウイルスに対して, ほぼ同一の感受性を示した. 螢光抗体法により, マレック病ウイルスと交差反応を呈したが, 同一反応ではなかった. 以上の研究により, 本ウイルスは, マレック病ウイルスと異なり, Witter らの報告したシチメンチョウ・ヘルペスウイルスと性格がほぼ一致するものと考えられる. 本研究の要旨は, 第71回(1971年4月)および第72回(1971年10月)日本獣医学会において報告した. 終わりに臨み, 電子顕微鏡による研究について, 御指導御協力下さった当研究室新居志郎助教授, 大阪医科大学内藤継也氏, ならびに本研究に際し, 種々御協力を賜わった兵庫県経済連藤田重夫部長, 小馬栄猛氏, 岸田満須夫氏, および柏原農協永井明男氏に深謝する.抄録全体を表示PDF形式でダウンロード (2366K) -
沢崎 坦, 広瀬 昶1974 年 36 巻 5 号 p. 421-426
発行日: 1974/10/25
公開日: 2008/02/13
ジャーナル フリーThe RR interval and the partial conduction time in the electrocardiogram of vertebrates were studied from the viewpoint of comparative cardiology. They were important for the physiological or patho-physiological approach to the clarification of cardiac function. RR, PQ and QT intervals and QRS complex durations were measured on 245 electrocardiograms recorded from twelve vertebrate species. Cardiological data obtained from some other experiments and the results reported by other workers were referred to in discussion. Positive correlations were recognized in general among the partial conduction times in one another. However, positive correlations of RR-PQ, RR-QT and RR-QRS and a negative correlation on a bilogarithmic scale between the heart weight and the heart rate per minute in resting state were recognized only in homothermic animals. A relationship between the submaximal increasing ratio of heart rate and the heart rate in resting state was indicated approximately by a hyperbolic function. From the results obtained, it was presumed that the original pattern of the cardiac conduction system might be manifested in poikilothermic animals, and that animals showing a lower heart rate in resting state might be able to maintain a capacity of increasing the heart rate greater than those showing a high heart rate.抄録全体を表示PDF形式でダウンロード (608K) -
湯浅 亮1974 年 36 巻 5 号 p. 427-432
発行日: 1974/10/25
公開日: 2008/02/13
ジャーナル フリーWhen a very effective glucosidurogenic compound, eugenol, was administered to the rat, the following results were obtained on the metabolic response of the glucuronidation system. 1) After a single oral administration of eugenol, a large amount of ether-type glucuronide excreted was found in the urine collected over the 0-12 or 12-24 hour period after the administration. It was reduced rapidly to a normal level. 2) After the same administration, the specific activity of UDP-glucuronyltransferase in the liver started to increase. gradually at 12 hours, reaching its maximum value at 48 hours, and then declined slowly. Liver weight showed a tendency to increase in percentage to initial body weight since 36 hours after the administration. 3) UDP-Glucuronyltransferase activity enhanced by eugenol was localized in microsome in the same manner as basal activity. These results indicate that rapid glucuronide formation and excretion of the administered glucosidurogenic foreign compound took place in the living body, and that the inductive adaptation of UDP-glucuronyltransferase occurred subsequently to the administered compound in liver microsome. It was postulated that an effective glucosidurogenic compound might act in vivo as a 'substrate inducer' on liver UDP-glucuronyltransferase.抄録全体を表示PDF形式でダウンロード (594K) -
及川 弘, 川口 陽資1974 年 36 巻 5 号 p. 433-440
発行日: 1974/10/25
公開日: 2008/02/13
ジャーナル フリーコクシジウムに感染したヒナの摘出消化管を用い, Magnus 法によるアセチルコリン反応性を検討した. すなわち, Eimeria tenella および E. acervulina をそれぞれ経口的に一回感染させ, 臨床症状の極期と回復期について, 消化管各部位における原虫の寄生状態との関連において, 消化管の収縮反応を調べた. E. tenella 感染により, 宿主の盲腸の収縮反応は著しく低下し, 一部では弛緩する例もみられた. これは原虫が, 盲腸の粘膜筋板に至る粘膜下組織の層に寄生することと深い関係があるものと考えられる. しかし, 収縮反応は寄生部位でない小腸下部でも低下し, 回復期の小腸上部では逆に亢進した. E. acervulina 感染では, 原虫は十二指腸から小腸中部にかけて, 絨毛上皮細胞内に寄生する. 小腸上部の収縮反応は, 症状の極期には亢進し, 回復期には低下する傾向にあった. 感染部位でない盲腸や直腸では影響がみられなかったが, 小腸下部では低下した. このように, 消化管各部位の収縮反応性は, 原虫の寄生によって直接的な影響を受けた. また, E. tenella 感染と E. acervulina 感染では, 影響の受けかたに相違がみられた. 症状の軽重(感染量の多少)と収縮反応との関連性は, E. tenella 感染の回復期の小腸以外では認められなかった. 両種の寄生部位でない小腸下部で, 収縮反応が著しく低下したこと, E. tenella 感染により, その寄生部位でない小腸上部にも影響が認められたことは, 感染の影響が決して局部的でなく, 消化管全体の運動能にも及ぶことを示唆しているものと考えられる.抄録全体を表示PDF形式でダウンロード (841K) -
古郡 浩, 山田 豊1974 年 36 巻 5 号 p. 441-445
発行日: 1974/10/25
公開日: 2008/02/13
ジャーナル フリーFourteen piglets weighing 10.7 kg on the average were divided equally into two groups, a fasting and a feeding. The animals of the former group were forced to fast for 5 days, and those of the latter fed ad libitum for the same period. At the termination of the experiment, the ferrokinetics of 59Fe was determined. Although plasma volume was significantly smaller in the fasting group than the feeding group, there was no significant difference in plasma volume per kg of body weight between the two groups. There was no significant difference either in red cell mass between the groups, but red cell volume per kg of body weight was significantly greater in the fasting group. Thus, it seems likely that the plasma shift from intravascular to extravascular spaces due to weight loss resultant from fasting may have caused a decrease in plasma volume and hemoconcentration in the blood stream. A marked prolongation of plasma iron clearance and a diminution of the plasma iron turnover rate were observed in the fasting piglets. These animals had significantly less radioiron in red cells and more in the liver and spleen when examined 24 hours after intravenous injection of 59Fe. Nonheme iron concentration in the liver and spleen varied markedly from one individual to another. It was approximately half that of the growing pig. It is possible that an excess in count of red blood cells due to weight loss may cause a marked diminution in erythropoiesis, and that depressed erythropoiesis may restrict the amount of iron incorporated from the circulating iron into red cells, resulting in an increase in radioiron storage in the liver and spleen.抄録全体を表示PDF形式でダウンロード (551K) -
長谷川 篤彦, 臼井 和哉1974 年 36 巻 5 号 p. 447-449
発行日: 1974/10/25
公開日: 2008/02/13
ジャーナル フリーPDF形式でダウンロード (721K)
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