PseudorabicsvirusはHcrpcsGroupに属し,そのウイルス学的性状は,Hcrpcsvirusと同様に,詳細に検討されている.そしてまた,各種動物におけるPseudorabicsの自然発生例や実験的感染試験も,多くの研究者によって報告されている.しかし,ミンクやフェレットにおける本症の;試験研究に関する報告は,国内はもとより,国外においてもはなはだ少ない.最近われわれは,チェコスロバキアで牛用のワクチン・ウイルスとして報告された弱毒Buk株を入手する機会を得た.そこで,この株と,米国で広く本症の研究に供されているShope株とを使用し, ミンクやフェレットおよび本症に対してもっとも感受性が高い動物とされている家兄について,感染試験を行なった.木報では,それら感染動物の臨床的観察について記述した.ミンク,フェレットおよび家兎の総計70匹が,Shope株またはBuk株の種々のウイルス量を皮下接種された.それら動物で観察されたおもな臨,床症状は,羊・豚・牛にみられると同様に,痛痒症(いわゆる"Mcdi[ch")にともなう接種部位の掻傷,口からの流血,神経症状として四肢または全身の痙畢である.また重篤なものでは,死の直前に反弓緊張がみられた.これらの症状は,フェレットでは一般に軽く,ミンクでは,家兎と同様に,重度のものが多かった.感染動物の潜伏期は,2日から8田こわたっていた.症状の持続期間は短く,1日から3日で全例斃死した.この潜伏期間,およびウイルス接種から死までの期間と,接種ウイルス量との間には,逆相関がみられた吸入感染をうけたフェレットでは,痛痒症による掻傷はみられず,多くのもので,鼻孔からの出血がみられた.これら感染動物の体温は,急激な上昇よりも,むしろ下降を示し,とくに死の前日にその傾向が著しかった.なお,これらの感染動物でみられた臨床症状,潜伏期間および症状持続期間においては,弱毒Buk株とShopc株との間に,著明な差は認められなかった.

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In the previous investigationt, tlae authors studied a respiratory disease of rats andshowed that some species of Mycoplasma miglat be associated with the disease. Thepresent experiment was carried out over a period from December, 1965, to July, 1966, to study the difference in contaminatiorn with Mycoplasma due to breeder farm stock, age, and sex of rats. The results obtained are summarized as follows.l. Only the rats of breeder SD were found to be free from Mycoplasma amongthose of the six breeder farm stocks examined. The rats of breeders C and D werefound to be contaminated witlt Mycoplasnna at a higher rate than tlnose of breeders A, B, and E. Amorig the breeder farm stocks of A, B, and E, however, no difference wasrecognized in the rate of isolation of Mycoplasma.2. Among the rats of breeders A, B, and E, the adults were found to be contami-nated with Mycoplasma at a higher rate than the young. Among the rats of breeder D, no age difference was found.3. In adult rats, Mycoplasma was isolated from males at a higlaer rate tlaan females.No sex difference was found, however, in young ones.4. The rate of isolation of Mycoplasma from the respiratory tract was the higl?estin the nasal cavity, the second highest in the bronchi, and the lowest in the lung.5. When the data were examined statistically, no relationsltip was recognizedbetween Mycoplasma and the gross Iesion in the lung. FurtI?ermore, a relationsltip wasdiscussed between this gross lesion and Mycoplasma or clinical findings in all therespiratory system.

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The amnatomy of tlae distribution of the N. olfactorius in the fowl is described andillustrated in detail on the basis of studies on 8 complete cases of macroscopical dis-section and 9 cases of microscopical observation. Ilistological materials were fixed inBouins fluid, 100% alcohol, or 50% pyridin. They were treated by the Bielschowskyor Cajal silver impregnation methods, and made to paraffiun sections 2Os30 p thickness.The nomenclatures of the N. olfactorius given in literature on the bird are discussedat full length. The following findings warrant emphasis.The N. olfactorius arises with about 30 fine rootlets at the rostral end of the Bulbusolfactorius, and runs into the ocular cavity passing through the Foramen (nervi) olfac-torii. It then runs forward along the dorsal edge of the orbital septum. At the levelof the caudal pole of the nasal bone, the N. olfactorius divides into two branches (Figs. 1, 2), which are named he Rami interni and Rami externi (Figs. 2, 4). The formerruns ventrolaterally into the posterior turbinate bone (Figs. 4, 5, 6) and finally dividesinto filaments, which are distributed over the dorsal mucosal surface of the turbinateprocess.The latter runs forward into the dorsal wall of the posterior turbinate bone (Figs. 4, 6), and is distributed oxzer the dorsal mucous membrane of the turbinate bone, dividinginto filaments.Neither the N. terminalis nor the nasoxromeral organ, or Jacobsons organ, is foundin the adult fowl. This result is the same as already mentioned in literature.

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Viable counts were made of the fecal flora of 4 pigs fed a fermented-Chlorella dietin exchange for a commercial diet.l. Streptococci, veillonellae, coliforms, and staphylococci were reduced after theexchange of diet and particularly showed a great decrease in count in and after thesecond week. Accordingly, it is clear that these four groups of bacteria were suppressedby the fermented-Chlorella diet.2. Yeasts began to show a considerable increase in the third week after the exchangeof diet.3. Pediococci were frequently found in considerable numbers in the feces in thefourth week and the last week after the exchange of diet. This may indicate that somechanges have occurred in the contents of the digestive tract to such an extent as toallow these bacteria and yeasts to survive or multiply.4. The administration of the fermented-Chlorella diet had no significant influenceon the quantities of lactobacilli, bacteroides, and biftdobacteria.5. Anaerobic streptococci (principally Peptostreptococcus elsdenii) were frequentlyfound in considerable numbers during the experimental period.

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豚痘感染と見なされる豚皮膚細胞内に,2種の封入体が見いだされた.一つはマトリックス型封入体で,すてに豚痘において述べられているものである.中等度の電子密度をもつ顆粒状ないし微細な線維性の領域と,発育型および成熟型ウイルス粒子より形成されている.他の一つは古典的封入体で,電子密度の高い無構造・均質性の物質よりなる限界明瞭な種々の大きさの領域より形成されている.後者は,豚痘においては報告されていないが,これは牛痘またはエクトロメリア痘に見られる古典的封入体に一致するものである.これら封入体は,光学顕微鏡的に,豚痘皮膚に認められる封入体に一致する.後者の封入体は,ヘマトキシリンおよびエオジン染色において,強く好放性を呈する均質性封入体に該当する.前者封入体は,弱好酸性または好中性で原形質との境界不明瞭な不整形封入体に合致するものである.これら均質性封入体は,ワクシニア・ウイルスによってはまれにしか形成されないといわれている.この事から,豚痘において均質性封入体が見いだされる場合には,それは豚固有のボックス・ウイルスによって引き起こされた疾患と考えてよいであろう.

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Interrelationships among environmental temperature, physiological conditions ofanimal, milk yield, and milk composition were discussed in lactating Holstein cows.The present experiment was carried out on the Livestock Farm, University ofTokyo, in Ibaraki Prefecture from May 26, 1964, to May 25, 1965, using 7 Holstein cows.To estimate the physiological conditions of animals, the following items were examined:the heart rate, the respiration rate, the rectal temperature, tlae packed cell volume, thewhite blood count, and the differential leucocyte count. The data obtained were analysedstatistically by using the technique of time serial analysis.The results obtained were summarized as follows.l) The milk yield was affected by ambiextt temperature. When the ambient ten-perature was higher than 20C, the milk yield decreased, being inversely proportionalto the rise of temperature. However, in autumn and winter when the ambient tem-perature was lower than 2OC, the milk yield increased in proportion to the rise oftemperature. This relationship was not clear in spring.2) Among those physiological phenomena, which were affected by ambient tem-perature, the heart rate, the rectal temperature, and the packed cell volume were closelyrelated to milk yield.3) Partial corrlation coefficients were calculated in order to eliminate the influenceof temperature from these complicated interrelationships. The heart rate was directlyproportional to the milk yield. The rectal temperature and the packed cell volumewere inversely proportional to the milk yield.4) The milk yield was influenced distinctly by the change of environmental term-perature. In this case, the milk yield was affected also by the blood circulation andwater metabolism.It was presumed that these functional changes showing the seasonal fluctuationmight be direct causes of variation in milk yield.

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