ToxoPlasmagondiiの組織培養過程にメジウム中に放出される補体結合(CF)抗原物質,すなわちTC抗原は,硫安の半飽和によりその半量が,全飽和によりその残余のものが,それぞれ完全に沈降した.これはSAB工Nにより改変されたWARRENandRUSSの鶏胎漿尿膜からのCF抗原(CAM抗原)とも,またHooKandFARBERが報告した虫体浸出液からのCF抗原とも,沈降態度が若干異なる.TC抗原は保存性が高く,100°01時間の加熱によっても,抗原性の低下は全く認められない.しかし硫安その他による精製の進行に伴ない,熱抵抗性は減弱し,またトリプシンにより不活化される.DEAEセルローズによるカラム・クロマトグラフイーの成績によれば,これに吸着される抗原分画と,吸着されない分画とに二分される.さらに前者は2~3の抗原群にわかれるが,これらはおおむね0.05~0.6Mの間に溶出してくる.DEAEセルローズ非吸着の抗原分画は,若干の溶血作用を示すのでCF抗原として不適当であるが,TC抗原や硫安精製TC抗原にはかかる作用は認められない.吸着溶出された抗原群は,人工感染豚および兎血,清に対し,TC抗原やCAM抗原とほぼ類似の反応態度をとるが,これと必ずしも一致しない成績を示?す場合が観察された.いずれが特異反応であるかについては,現在のところ明らかではない.組織培養法で収獲した虫体からは,塩掖抽出法,凍結融解法,熱抽出法,または蒸留水抽出法のいずれによっても,容易にCF抗原を抽出することが可′能である.この場合,抽出に用いる虫体浮遊液は,少なくとも8×1077ml以上の濃度の虫体を含有する、ことが望ましい.凍結融解法は7回以上,熱抽出は,100°CI時間程度,行なうのがよい.組織培養虫体からのCF抗原は,従来のものに比、べ比較的純粋な,しかも抗体,体液等生体からの悪感作を受けることがない虫体を出発材料とすることができることと,TC抗原同様,100°CI時間の加熱に対しても安定で,保存性にすぐれていることなどから,従来のものに代りTC抗原同様に,今後大.いに利用されるべきものと考えられる.

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The authors previously reported the clostridial flora in the feces and alimentary tractsof various animals, stating that there were many unidentified strains among their isolates.In this study, the clostridial flora in soil was investigated. Twenty-seven samples werecollected from 7 prefectures of the northern part of Honshu, Japan, i.e., Aomori, Iwate, 1VIiyagi, Akita, Yarnagata, Fukushima, and Niigata Prefectures.The results obtained were as follows.I. A total of 217 strains were isolated frorn these soil samples. Of the isolates, 107strains were identified as the following II species of the genus Clostridium in accordancewith Bergeys Manual.sporogenesperfringensmultfermentansparabotulinumbutyricum7Z o vy Zbotulinumfallaxtetanoidescochleariumiodophilumsphenoides110 strains50 stranns21 strains14 strains8 strains3 stratns3 strauns2 stramns2 strauns1 strain1 strain1 strain1 strain(50.7%) were unable to be identified.

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第II報で,II・III群ファージに対する感受性によって,ひな白痢菌の亜型が同定できることを述べた.これについて,単に溶菌現象の面だけでなく,その特異性の本質を明らかにすることは,ファージ対亜型の関係を一層明瞭にするものと考えられ,著者が意図するファージと,ひな白痢菌122抗原のFormVariationとの関係を究明する上に,重要な資料となる.本試験では,ファージ・レセプターを明らかにする目的で,各亜型の菌体より分離した脂質,多糖体,蛋白質,およびこれらを抽出した残りの菌体を用いて,溶菌阻止作用を調べ,次の成績を得た.I)ファージ感受性菌が所有する抗原特異多糖体が,当該ファージの溶菌性を阻止する.すなわち,II群ファージはSおよびV型菌の,III群ファージはV型菌の多糖体によって,溶菌性が阻止された.一方,IIおよびIII群ファージに溶菌されないI型菌より得た分画は,両群のファージに対し,全く活性を示さない.2) ファージ爾性菌の多糖体は,ファージに対する溶菌阻止作用を消失した.これは耐性菌交叉試験の所見と良く一致する.3)正常な菌体抗原が失われているR型菌は,ファージ感受性をもたない.4)各亜型のひな白痢菌,ファージ耐性菌および鶏チフス菌の多糖体を構成する単糖は,定性的にはすべて同一であって,それぞれGlucosaminc,Galac一toscクGlucosc,Mannose,Xy10sc,RhamnoscおよびTyvcIoscが検出されるが,ファージ感受性を決定する特異的な単糖の存在は認められない.したがって,ファージ・レセプターは,単糖の立体構造の差によって決定するものと考えたい.本論文は,北海道大学審査学位論文で,規定に基づ1公表する.

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During a period from 1960 to 1963, a total of 568 serum samples were collected from209 cattle>70 swine>120 goats>28 dogs>and 186 persons in the Kyushu district. Theywere tested for the presence of neutralizing antibodies against two types of bovine entero-viruses which had been isolated from the feces of apparently healthy cattle in Japan. A testwas considered positive when the antibodies in the serum tested neutralized 100 TCID../... mlof viruses in a dilutiorr of l : 10.I. Neutralizing antibodies against type I and type II viruses were found in 93.8 and68.8%, restcectivelv>of the bovine serum samtcles collected from Kurnamoto Prefecture, in33.3 and 0.9% of those collected frorm [VIiyazaki Prefecture>and 66.7 and 43.3% of thosecollected from Saga Prefecture.2. Antibodies against type I and type II viruses were found in 35.7 and 56.3%, respectively, of the swine serum samples collected from Kumarnoto Prefecture and 66.0 and77.8% of those from Oita Prefecture.3. Positive tests with type I and type II viruses were given by 25.0 and 22.5%, respectively, of the goat serurn samples collected from Kumamoto Prefecture.4. No demonstrable antibodies were found in 28 dogs collected from KurnamotoPrefecture.5. Type I and type II viruses were neutralized by 78.5 and 94.3%, respectively, ofthe human sera collected in Kumamoto Prefecture.6. Positive reaction to these viruses was shown by cattle kept in Aso County, Kumamoto Prefecture, where the viruses had been isolated, at 21 final dilution of I : 160 to1 : 640, and by those kept in another county of the same prefecture at a dilution of 1 : 20to 1 : 160.7. In this survey>bovine, hunnant and swine sera frequently>and goat serum occasion=ally, reacted with bovine enteroviruses, because of the presence of neutralizing antibodies.8. No inhibitory activity upon the two types of bovine enLerovirttses was found in thebovine, goat, swine, and human sera examined.

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In their previous investigation, the authors studied the normal bacterial flora of therectal feces in the healthy pig, and particularly clarified changes in the species and numbersof organisms appearing in the rectal feces with the advance in growth of the pig. Ill thepresent investigation, experiments were carried out to determine what changes would occurin the normal bacterial flora of the rectal feces and contents of intestinal tract of the pigwhen large doses of various antibiotics were aclrrninistered to this animal.The results obtained are summarized as follows.1. No remarkable changes occurred in the normal bacterial flora after the administ-ration of a single dose of any antibiotic. Only Escherichia coli and Lactobacillus organismsshowed a decrease in number for several days after the single administration. Accordingly, it was made clear that the composition of tlte normal bacterial flora was of rather solidstability.2. It was extremely difficult to remove all Line bacteria from the bacterial flora ofthe rectal feces even by means of the consecutive administration of large doses of antibiotics.Such type of administration finally allowed Streptococcus exclusively to multiply in therectal feces.3. Characteristic changes were observed in the bacterial flora of each portion of thedigestive tract in a pig administered consecutively with large doses of antibiotics. Whenthe relationship between the section of the alimentary canal and the number of microbialspecies isolated was examined>the section covering tlre duodenum through the cecum wasfound harboring Yeasts at 5 to 8, as expressed by Log count per gram of sample, and theduodenum and ileum eontained Bifidobacterium at 4.5 and 8, respectively. The value ofStreptococcus detected was 10 in the rectum and decreased gradually as the intestinalcanal was followed upward, reaching 5 in the duodenum.4. It is very diflicult to detect any abnormality on the part of the animal body byexamining the bacterial flora of the rectal feces alone. In order to attain these p

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