日本獸醫學雜誌(The Japanese Journal of Veterinary Science)
Online ISSN : 1881-1442
Print ISSN : 0021-5295
ISSN-L : 0021-5295
36 巻, 4 号
選択された号の論文の8件中1~8を表示しています
  • 川村 清市, 安田 純夫, 田山 春實, 茂木 久佳, 高瀬 勝晤, 小笠原 成郎
    1974 年 36 巻 4 号 p. 285-290
    発行日: 1974/08/25
    公開日: 2008/02/13
    ジャーナル フリー
    In order to improve the separativity of serum proteins of cattle in electro-phoresis on cellulose acetate membrane, the constituents and the concentrations of buffersolution were investigated in this experiment.Better results of fractionation were obtained from a barbital calcium buffer (pH 8.6)wltich consisted of 2.0 g of calcium lactate and l .0 Z of 0.06 M barbital buffer than from thestandard barbital buffer. By using that buffer, a-globulin (gl) in the serum of cattle wasdivided into two fractions, a.-gl and a.-gl, and the separation of $-gl and 7-gl was clearlyvisible.The percentages of the serum protein fractions in healthy dairy cattle were determinedand the normal range of each fraction was calculated. A significant increase in ex.- anda.-gl was recognized in various diseases, including liver disturbances, nephritis and someinfectious diseases.It is considered that in making the clinical diagnosis of some disease, the barbital calciumbuffer is worthy of applying to the fractionation of serum proteins of cattle by electro-phosesis with cellulose acetate membrane. By using this buffer, the serum protein fractionswere separated distinctly, and a.- and a.-gl divided clearly and increased significantly inseveral diseases.
  • 小黒 捷義, 池田 三義
    1974 年 36 巻 4 号 p. 291-298
    発行日: 1974/08/25
    公開日: 2008/02/13
    ジャーナル フリー
    An experiment was carried out to observe the movement of the gastrointes-tinal content in the chncken. In it, l mZ, 3 ml or 6 ml of the marker, Na."Cry., was admin-istered pes os to the proventriculus of a restrained conscious chicken. The distribution ofthe marker in the gastro-intestine was investigated in each group.l. In the proventriculus, the marker scarcely existed immediately after administrationwith the lapse of time.2.. On the other hand, when l mZ of the marker was administered, its content in thegizzard was larger 40 minutes than 20 minutes after administration, while its content inthe central segment of the small intestine was smaller 40 minutes than 2, 0 minutes afteradministration. The decreased quantity of its content in the central segment of the smallintestine was almost equal to the increased quantity of its content in the gizzard 40 minutesafter administration, while there was no difference in quantity between the content in theduodenum and the upper segment of the small intestine 20 minutes after administrationand that 40 minutes after administration. The same results were obtained when 3 mZ and6 ml of the naarker were administered and observation was made 40 and 60 minutes afteradministration.3. These results suggested that some portion of the content of the small intestinemight have regurgitated into the gizzard through the upper segment of the small in-testine and the duodenum.
  • 金本 勇, 原 崇
    1974 年 36 巻 4 号 p. 299-309
    発行日: 1974/08/25
    公開日: 2008/02/13
    ジャーナル フリー
    In a male Maltese two months of age weighing 700 g, patent ductus arteriosuswas diagnosed, based on the results of clinical examinations. Operation was carried out onthis case with excellent results. Characteristic findings of clinical examinations, operation, and postoperative course are summarized as follows.l. Since about l month of age, attacks of congestive heart failure had occurred fre-quently with severe dyspnea and collapse.2. Palpation revealed characteristic, intense and continuous thrill over the precordialregion.3. Auscultation revealed loud machinery-like murmur over the cardiac base, especiallyin the aortic, pulmonic and tricuspid valve areas.4. X-ray findings in the ventrodorsal radiogram revealed the characteristic "dancing"of the main pulmonary artery and a leftward bulge of the descending aorta with the peculiarteardrop shape of the heart.5. PCG findings revealed systolic murmur which was of diamond type between heartsounds I and II.6. ECG findings revealed deviation of the electric heart axis, dilatation of the bilateralatria and ventricles, and abnormalities in the formation and conduction of stimuli.7. After thoracotomy at the left 3rd intercostal space, the ductus arteriosus was con-firmed between the descending aorta and the bifurcation of the main pulmonary artery.It was remarkably broad, about 0.5 cm in length and 0.8 cm in diameter, and cylindrical inshape.8. On the first postoperative day, hemorrhage was noted in both anterior ocular chambers. It disappeared completely within 5 days after operation.9. On the 30th posLoperative day, all abnormalities disappeared, except a slight dilataLion of the left ventricle, in clinical examinations.
  • 早津 栄蔵, 杉山 晴子, 川久保 安朗, 木村 道子, 吉岡 守正, 西山 保一
    1974 年 36 巻 4 号 p. 311-319
    発行日: 1974/08/25
    公開日: 2008/02/13
    ジャーナル フリー
    Immunity in chickens with killed organisms of Mycoplasma gallisepticumwas evaluated according to the lesion grade of airsacculitis, the retention of M. gallisep-ticum in organs and histopathological findings. Killed M. gallisepticum did not provideany resistance when intramuscularly administered either alone or in admixture withadjuvants. An increased resistance to infection, however, was observed when it was administered into the respiratory tract in a form of aerosol or by intratracheal injection.A partial immunity was maintained at a significant level for at least 3 months. Theseresults suggest that protection of chickens against M. gallisepticum infection may be pos-sible when the killed vaccine is applied directly to the lower respiratory tract of thechickens. The suggestion is supported by the histopathological findings of specimens col-lected from immunized birds. Little correlation was seen between serological serum titerand resistance to challenge.
  • 及川 弘, 川口 陽資, 中元 弘次, 角田 清
    1974 年 36 巻 4 号 p. 321-328
    発行日: 1974/08/25
    公開日: 2008/02/13
    ジャーナル フリー
    Field surveys on coccidial infection in Japanese broilers were performed with187 fecal samples collected from 29 prefectures in March (spring season) and June (rainyor summer season) in 1973.Species identification was carried out by two methods; examination of oocysts morpholo-gy and chicken passage test. Based on the morphological characters of the oocyst type, genus Eimeria was classified into four types, acervulina, brunetti, maxima, and tenella.Oocysts were detected from 73% of the samples tested in March. Single and mixed infec-Lion with the acervulina type were found in 66% of all the samples tested, or in 90% of theinfected samples, and those with the tenella type in 28% or in 38%. The maxima typewas detected at a high rate. Similar results were obtained in the survey conducted in June.Neither seasonal nor regional difference was observed in the distribution of the oocysttypes. A high rate of infection and large OPG values were shown by chicken from 31 to70 days of age.It was confirmed that Eimeria acervulina and E. maxima could be identified from themorphology of oocysts. The chicken passage test was required to distinguish the oocystsof E. tenella, E. necatrix, and E. praecox from those of tenella type in the fecal samples.Those three species were distributed all over Japan without restriction.No E. brunetti was detected by either of the methods used.
  • 板倉 智敏, 安場 正子, 五藤 精知
    1974 年 36 巻 4 号 p. 329-340
    発行日: 1974/08/25
    公開日: 2008/02/13
    ジャーナル フリー
    Eighteen broiler chickens affected spontaneously with inclusion body hepatitiswere studied histopathologically. Fatty degeneration and formation of intranuclear inclusion bodies in the hepatic cells were characteristic of this disease, as has been pointed outby previous workers. Various types were noticed in the inclusions. They were dividedroughly into two types, eosinophilic and basophilic, on the basis of stainability. Inclusionsof the former type were found in cells showing fatty degeneration and generally numerousthroughout the liver in all the birds examined, whereas those of the latter type were fewand found in cells hardly affected.Lesions of nephrosis, pancytopenia in the bone marrow, and atrophic changes in thebursa of Fabricius were consistently observed in the birds examined. It was emphasizedthat should be attention paid to these lesions, especially those in the bursa of Fabricius, in this disease, in addition to the hepatic changes.Two concurrent diseases, chronic respiratory disease and bacterial (mainly Escherichiacoli) infection, occurred in many cases. It was apparent that the latter complicated thecondition.
  • 片江 宏巳, 中田 勝久, 高瀬 善行, 清水 当尚
    1974 年 36 巻 4 号 p. 341-350
    発行日: 1974/08/25
    公開日: 2008/02/13
    ジャーナル フリー
    Spectinomycin (SPE) is an antibiotic isolated from Streptomyce.s spectabilisNRRL 2792 and S. flavopersicus sp. n. NRRL-B-2820. It was active against most Grampositive and Gram-negative bacteria in vitro. Mycoplasrna gallisepticum was especiallysensitive to this antibiotic. Most strains of Escherichia coli and the genus Salmonellafreshly isolated from sick poultry were sensitive to SPE, showing no cross-resistance toother antibacterials. The activity of SPE was hardly affected by the addition of horseserum or soditum cholate, but was weakened by an acidic pH or a large bacterial inoculum.When 5-day-old chickens were administered subcutaneously with a single dose of 100 mg/kg of SPE, the average plasma level of SPE attained a peak of 121 pg/ml 30 minutes afteradministration and then descended gradually to attain 9.8 jtg/ml 6 hours. When chickenswere administered orally with a single dose of 200 mg/kg of SPE, the average plasma levelreachecl a plateau of about 7, pg/ml from 3 to 6 hours after aclministration. In the systemicinfection with E. coli or Salmonella species in mice and chicken, SEE was effective byparenteral administration. Its efficacy was also observed in the air-sac infections with M.gallisepticum in chickens.From these results obtained, it is concluded that SPE may be worth being applied to thecontrol of some animal diseases.
  • 平井 克哉, 鈴木 義孝, 加藤 直樹, 八神 健一, 三好 昭義, 馬淵 良生, 和 秀雄, 稲垣 晴久, 大槻 公一, 坪倉 操
    1974 年 36 巻 4 号 p. 351-355
    発行日: 1974/08/25
    公開日: 2008/02/13
    ジャーナル フリー
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