日本獸醫學雜誌(The Japanese Journal of Veterinary Science)
Online ISSN : 1881-1442
Print ISSN : 0021-5295
ISSN-L : 0021-5295
40 巻, 3 号
選択された号の論文の13件中1~13を表示しています
  • 三輪 憲永, 近森 憲助, 首藤 文栄, 久保 周一郎
    1978 年 40 巻 3 号 p. 265-274
    発行日: 1978/06/25
    公開日: 2008/02/13
    ジャーナル フリー
    Porcine erythrocyte adenylate kinase showed three components, AKa, AKb and AKc, designated on the basis of the distance of migration from their origin to the anode in starch gel electrophoresis. By isoelectrofocusing, 96% of the total erythrocyte adenylate kinase focused at pH 9.25, and this predominant enzyme form corresponded to AKa in starch gel electrophoresis. The predominant enzyme form, AKa, was purified at approximately 31, 000 fold from the porcine erythrocyte in an overall yield of 45% by affinity chromatography on a column of Blue Dextran-Sepharose 4B, substrate elution from a column of phosphocellulose, and by isoelectrofocusing. The purified AKa had a specific activity of 2, 100 units per mg of enzyme and migrated in gel electrophoresis as a single band having a molecular weight of 21, 500. The amino acid composition of AKa was the same as that of the skeletal muscle enzyme. Peptide mapping showed a correspondence of all spots of the tryptic peptides from the erythrocyte and skeletal muscle enzyme preparations. These results strongly suggest that the predominant adenylate kinase in porcine erythrocyte is identical to porcine skeletal muscle adenylate kinase.
  • 鹿江 雅光, 井土 裕児, 戸田 光敬
    1978 年 40 巻 3 号 p. 275-279,281
    発行日: 1978/06/25
    公開日: 2008/02/13
    ジャーナル フリー
    A survey was conducted on the occurrences of rumen parakeratosis, rumenitis, traumatic reticulitis, and reticular and hepatic abscesses among 492 slaughtered cattle in Yamaguchi and Fukuoka Prefectures, Japan. These diseases occurred to 59 animals (12.0%), 83 (16.9%), 26 (5.3%) and 25 (5.1%), respectively. Hepatic abscesses were detected from 18 (12.0%) of 149 animals with rumen parakeratosis and/or rumenitis, 3 (11.5%) of 26 with traumatic reticulitis, 1 (12.5%) of 8 with reticular abscesses, and 3 (1.0%) of 309 with no disorders. Fusobacterium necrophorum was detected from 9 of 20 cases of rumen parakeratosis, 12 of 20 cases of rumenitis, 1 of 10 cases of unaffected ruminal tissue, and all of the cases of reticular and hepatic abscesses. The viable count of F. necrophorum was 104 to 106 per gram in the cases of rumen parakeratosis, 103 to 107 in those of rumenitis, and 105 in that of unaffected ruminal tissue.
  • 斉藤 学, 中川 雅郎, 木下 邦明, 今泉 清
    1978 年 40 巻 3 号 p. 283-290
    発行日: 1978/06/25
    公開日: 2008/02/13
    ジャーナル フリー
    Etiological studies of pneumonia in mice were made on natural outbreaks, with special reference to P. pneumotropica, M. pulmonis, C. kutscheri and Sendai virus. Pulmonary consolidation and suppurative lesions with bronchiectasis were rarely detected in mice from either colonies free from all the organisms examined or those infected with P. pneumotropica and/or M. pulmonis. In colonies infected with Sendai virus, however, the lung lesions were demonstrated in 1.7 to 78.3% of mice, regardless of the presence or absence of other pathogens. Considerably high incidence of the pulmonary lesions, 7.6 to 20.0%, were observed in mice from all colonies infected with P. pneumotropica, M. pulmonis and Sendai virus. From the pulmonary lesions, M. Pulmonis was isolated at high frequencies and in a larger number, while some were detected from grossly normal lungs. With P. pneumotropica, the results were similar but the difference between the affected and normal lungs was less remarkable. Mice harboring both M. pulmonis and P. pneumotropica in their lungs were mostly positive for Sendai virus antibody. C. kutscheri was rarely isolated from pulmonary lesions in the present cases.
  • 寺門 誠致, 上田 久, 菅原 天晴, 伊佐山 康郎, 小山 敬之
    1978 年 40 巻 3 号 p. 291-295
    発行日: 1978/06/25
    公開日: 2008/02/13
    ジャーナル フリー
    Brucella canis strains isolated from house dogs, stray dogs and beagles in breeding or experimental colonies were examined for their susceptibility to 38 antibacterial drugs by an agar dilution method. All of 90 strains tested were found to be highly susceptible to tetracyclines and aminoglycoside antibiotics. These were also susceptible to chloramphenicol, spectinomycin, rifampicin and sulfonamide. However, in these isolates, decreased susceptibility was found to cephalosporin antibiotics and nalidixic acid, and almost insensitivity to peptide antibiotics and cycloserine. On the other hand, 22 (24.4%) of 90 strains tested were found to be multiply resistant to macrolide antibiotics, some of penicillins, novobiocin and lincomycin. These multiple drug-resistant strains were isolated from stray dogs and beagles in various districts, indicating that the prevalence of such resistant strains is relatively wide-spread in dogs in Japan.
  • 和 秀雄
    1978 年 40 巻 3 号 p. 297-307
    発行日: 1978/06/25
    公開日: 2008/02/13
    ジャーナル フリー
    Nine female Japanese monkeys were ovariectomized. The exact time of ovulation was determined in them by laparoscopic observation of ovaries. Accurately dated ovaries were examined histologically. Some slight folds and some large lutein-cell-like granulosa cells were seen in a pre-ovulatory follicle. They suggested that a luteinization might have started in an ovary before ovulation. The formation of a new corpus luteum progressed gradually during the first 40 hours after ovulation and was accelerated during the following several days. The data obtained in this study differed in some aspects from those in a study of rhesus monkeys. An invasion of the granulosa layer by blood capillaries was seen in the corpus luteum 15 hours after ovulation and the capillaries had already rcached a cavity 37-40 hours after ovulation. Mitotic figures were observed in granulosa and theca interna cells until 43-47 hours after ovulation. It was not impossible to distinguish granulosa cells from theca interna cells in any stage, although it was difficult to do so in some stage.
  • 江島 博康, 黒川 和雄, 阿部 賢治, 柄沢 勉, 志方 俊夫, 池本 卯典
    1978 年 40 巻 3 号 p. 309-313
    発行日: 1978/06/25
    公開日: 2008/02/13
    ジャーナル フリー
    Seventeen blood samples of chimpanzee were examined for the presence of homologous antibody. All of them contained anti-B but not anti-A antibody. They also possessed A-like antigen on the red cell membrane. After complete absorption of the antibody by human B and AB red cells, the existence of homologous antibody was demonstrated in chimpanzee serum by the bromelin method. This antibody was detected from eleven of the 17 samples tested and tentatively designated anti-Ch antibody. With this antibody chimpanzees could be divided into two types, Ch (+) and Ch (-). Of the 17 chimpanzee samples, two were of Ch (+) type and the other fifteen of Ch (-) type.
  • アリアス-イバロンド ヘスース, 見上 彪, 山本 博, 古田 有一, 石岡 佐平, 岡田 幸助, 佐藤 儀平
    1978 年 40 巻 3 号 p. 315-323
    発行日: 1978/06/25
    公開日: 2008/02/13
    ジャーナル フリー
    A virus isolated from the brains of young Japanese sparrow-hawks suffering from an acute infection with a slight nervous symptom was shown to have the morphological, physical and chemical characteristics of a paramyxovirus. The virus was serologically related to Newcastle disease virus and Bangor virus but differed from them in some biological aspects mainly in hemagglutination (HA) activity. The HA reaction of the virus was temperature-dependent. The constant HA reaction was observed when tested at 4°C but not at room temperature or 37°C.
  • 鈴木 義孝, 嵩 終三
    1978 年 40 巻 3 号 p. 325-331,334
    発行日: 1978/06/25
    公開日: 2008/02/13
    ジャーナル フリー
    The spheroids in the brain and spinal cord were examined lightmicroscopically in 55 dogs (1 month to 18 years of age). Their shape varied from round to oval and was sometimes ellipsoidal or irregularly elongated, and the size ranged from 5 to 200μm in diameter. The spheroids were acidophilic and argyrophilic, histochemically positive for protein reaction but negative for nucleic acid reaction. As a new finding, PAS positive concentric round bodies were found within the spheroids in aged dogs. In the brain, the most constantly affected area was the dorsal column nuclei, especially in the gracile nucleus in the medulla oblongata. In the spinal cord, caudal segments from the lumbar to the cauda equina were noticed as the predilection sites, and the spheroids were more frequently found in the substantia intermedia hnd ventral column. The occurrence of the spheroids tended to increase with age and was marked in chronic wasting disease such as tumor cachexia and severe filariasis.
  • 其田 三夫, 高橋 清志, 田村 孝, 小岩 政照
    1978 年 40 巻 3 号 p. 335-339,341
    発行日: 1978/06/25
    公開日: 2008/02/13
    ジャーナル フリー
    1973年以来, Babesia gibsoni接種試験に供試中の犬の血液塗抹標本を鏡検しているうち, 赤血球にB. gibsoniのほかに, Haemobartonella canis様小体の付着が認められたので, さらに電子顕微鏡的観察を行った. その成績を総括すると, 次のごとくである. 1. 長崎市内で発生したB. gibsoni感染犬の血液を, 無処置および摘脾の20例の犬に逐次接種したところ, 種つぎのみのため継代接種した7例を除いた他の13例で, 赤血球の表面にH. canis様の寄生小体(H様小体と略す)の付着がみられた. 2. 本寄生小体の出現は, 接種後第3~8日, 平均第4.7日で, B. gibsoniの出現と同時か, またはそれよりやや早い. その後は, 個体により多様な出現状況を示した. 3. Giemsa染色した寄生赤血球を光学顕微鏡で観察すると, H様小体は赤紫色を呈し, 赤血球の表面に散在するか, または連鎖状に付着していた. 4. 電子顕微鏡で観察すると, H様小体は0.2~0.6μmの大きさで, 単位膜に包まれた顆粒状物質を含有し, 球菌状, 桿菌状またはドーナツ状を呈する. 赤血球の表層に生じた溝に深く入り込んで付着するので, 寄生赤血球は著しく変形していた. 以上の所見から, 今回の接種犬で観察された赤血球寄生小体は, H. canisと一致するものであり, わが国の犬にもH. canisの存在することが明らかとなった.
  • 高阪 精夫
    1978 年 40 巻 3 号 p. 343-348
    発行日: 1978/06/25
    公開日: 2008/02/13
    ジャーナル フリー
    VFAと赤痢菌感染, 発病との関係を知るための基礎資料を得る目的で, 正常サルと赤痢サルにおける消化管内のVFAとpHを比較検討し以下の成績を得た. 正常サル群では, 胃, 小腸下部にくらべて大腸内, とくに盲腸内のVFA総濃度は高かった. 一方, 赤痢サル群では胃を除いたすべての部位のVFA総濃度は正常サル群にくらべて約1/2低かった. そして, これらのVFA組成比は, 両群とも消化管の部位に関係なく, 酢酸の占める割合が一番高く, VFA総濃度の60~81%であった. また, 赤痢菌増殖の場である大腸内のpHについてみると, 正常サル群の平均pH (5.3~6.5)は赤痢サル群のそれら(6.8~7.1)にくらべて低い値を示した. 正常サル群の大腸内のVFA総濃度とpHとの間には負の相関が認められた(r=-0.39, P=0.05).
  • 池本 卯典, 桜井 幸男, 渡辺 嘉彦, 江島 博康
    1978 年 40 巻 3 号 p. 349-351
    発行日: 1978/06/25
    公開日: 2008/02/13
    ジャーナル フリー
  • 芹川 忠夫, 村口 武彦, 中尾 登, 入江 泰浩
    1978 年 40 巻 3 号 p. 353-355
    発行日: 1978/06/25
    公開日: 2008/02/13
    ジャーナル フリー
  • 木村 重, 清水 晃
    1978 年 40 巻 3 号 p. 357-359
    発行日: 1978/06/25
    公開日: 2008/02/13
    ジャーナル フリー
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