SDDS(2-sulfamoyl-4,4I-diaminodiphenylsu1一foneの投与により,RH株感染マウスの死期は著しく延長される.,また,これよりやや弱毒と思われるmodificdS-273およびmodificdBeverlcy株感染マウスに投与した場合には,90%以上が生残し,従来最も有効と考えられていたsuIfamo一nomethoxineに劣らぬ抗トキソプラズマ効果を示した.これらの実験成績をもとに,豚の本症に対する治療効果を検討した.自然感染豚より分離されたHG株を用い,感染(1×1071p)と同時に投薬を開始した場合(体重1kg当たり1日1回30mg,7日間筋肉内注射)は,発熱その他臨床症状ならびにparasitemiaの発現を完全に抑圧した.また発病の翌日から投薬を開始した場合にも,症状を軽快にし,かつ体内からの原虫を一掃することを知った.modinedBeverley株についても試験した.この場合,無処置対照群の1例と,治療群の全例が,62日後の検索において,原虫陰性の成績を示したため,本剤による治療効果を確実に判定することができなかった.投与回数や投薬量など,今後検討を要する事項が多く残されている.しかし本剤がトキソプラズマ症の予防治療剤として,きわめて有望なものであることは明らかである.

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1958年米国で,CI′andcllらによって猫鼻気管炎ウイルスが分離された。それ以来,猫腎皮質培養細胞を用いて,猫の呼吸器病ウイルスやOrphanウイルスなどの分離が,諸外国で報告されている。しかしわが国では,これらのウイルスの分離に関する報告は,全くないまま今日に至っている。著者らは,1963年春に子猫の呼吸器疾患の続発を認めた。そして眼・鼻より水様ないし膿様の分泌物を排して,斃死し,または安楽死させた猫18頭で,結膜・鼻腔・気管・糞便などの綿棒材料より,猫腎皮質培養細胞を用いて,ウイルスの分離を試みた.その結果,12頭(66.7%)より27株(46.6%)のウイルスが得られた.その大部分は結膜および上部気道より,また少数は肺および糞便から,分離されたものである.これらのウイルスを接種した猫腎培養細胞では,CPEは接種後24時間内に出現し,細胞は円形化するが,核内封入体は見られず,比較的大きなブラックを形成する.ウイルス価は105・25~107・5TC工D50/0.2mlである.全株とも,エーテル耐性で,ニワトリ赤血球凝集性は陰性であった.血清学的には,補体結合反応で分離株相互間に交叉がみられたが,中和試験では少なくとも2型に分類された.さらに,猫に対する病原性は,無症状に経過するものもあったが, 5~10日目ごろより眼分泌物排出・呼吸器症状などを呈し,のち治癒するもの,あるいはそのまま斃死するものなどがあった.そして接種猫のほとんどすべてから,ウイルスが回収された.したがってわが国にも,少なくと・も2つ以上の血清型に属するウイルスによる猫のウイルス性呼吸器病が存在することが明らかになった.

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The arterial system of the bovine uterus has been studied by Ellenbogen, Hanseland Asdell, Hilliger, Reuber and Emmerson, and Tsutsumi. Nevertheless, there havebeen only a few accounts on comparative studies regarding the caruncular region ofnulliparous heifer, multiparous cow, and pregnant cow. In the present investigation, atotal of 61 uteri from l 1 nulliparous heifers, 12 multiparous cows, and 38 pregnant cowswere used. Casts of arteries were prepared for this purpose by means of the intra-vascular injection of acrylic resin. Apart from the casts just mentioned above, tissueblocks were obtained from some uteri injected with resin. Transparent slices andcelloidin sections were also made. In addition to resin-injected materials, those injectedwith gelatine were also used. Paraffin sections of the caruncle were prepared fromnon-injected materials fixed in 10% formalin, Carnoys, Zenkers, or Bouins fluid, andstained with hematoxylin and eosin.The arcuate artery proceeded toward the antimesometrial margin within the stratumvasculare of the uterine wall. Many of them anastomosed with adjacent ones in arxarchfasluion at about 1/2 to 2/3 of the circumference of the horn from the mesometrialmargin. Finally, they terminated in an anastomosis with those of the contralateral rowon the antimesometrial margin. Meanwhile, many smaller arcuate arteries joined thearchfashioned anastomoses described just above. The arcuate arteries in a nulliparousheifer were nearly straight until they formed an anastomosing net, and thereafter theybecame strongly tortuous. On the other hand, those in multiparous cows were stronglytortuous throughout their course. Meanwhile, those arteries were almost straight ina cow which was in the second half of pregnancy.The arcuate artery gave ofl a radial artery to the caruncle and a branch to thecircular muscle. The radial artery usually give origin to a basal artery within thestratum vasculare, which supplied inner one-third of the circular muscle and stratum basale of the endometrium. The main trunk of the radial artery usually bifurcatedonce or twice in the endometrium and finally divided into many arterioles at the baseof the caruncle. These arterioles gave off many pre-capillary vessels which, in turn, continued to the subepithelial capillary net. TIIC radial arteries, especially those inthe caruncular region, were much more tortuous in the multiparous cow than in thenulliparous lueifer. Well-developed capillary networks were also present in the circularmuscle.In tlne second half of pregnancy, the arteries became adapted to the expansion ofthe uterus witlt the following modifications. The radial artery proceeded toward thetop of the peduncle, taking a course almost perpendicular to the base of it. Then, it divided into many arterioles, which proceeded to the bottom of the placentome, takinga fan-fashioned course. At the base of the placentome, these arterioles gave origin tocaruncular radial arteries, which were found to be rather thin at tlaeir origin and whichbecame thicker on their way to the surface of the placentome. The caruncular radialarteries in t[te placentome seemed to be an extension of the pre-capillary vessels whichwere invariably found near the surface of a caruncle in the nulliparous heifer, as wellas in the mu[tiparous cow.

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肉の血清学的鑑別には沈降反応が常用され,抗血清・血清,抗ヘモグロビン・血清,抗生肉浸出液・血清が使用されている.本方法により,一応,生肉は鑑別し得るが,動物分類学上近縁のものの間には交叉反応が示されることがある.一方,加工肉,特に缶詰のごとく,高度に加熱された肉を沈降反応により鑑別することは不可能とされている.著者らは,生肉および加工肉の血清学的鑑別方法を確立する目的で,馬,牛,緬羊,山羊,豚,犬,猫,および鯨の生肉浸出液,および強加熱肉浸出液(煮沸1時間後オートクレープ(110°C)1時間),ならびに非動化血清,煮沸血清(30分)および強加熱血清(前記と同様)の抗原性について検討した.I)生肉浸出液,非動化血清,煮沸血清,強加熱血清は抗原性を有していたが,強加熱肉浸出液は抗原性を示さなかった.2)生肉浸出液と血清との間に共通抗原が存在することが認められた.しかし生肉浸出液とヘモグロビンの血清学的関係は証明されなかった.3)生肉浸出液に対して最も種属特異性を示した免疫血清は抗煮沸血清・血清(カリミョーパン処理)であった.4)生抗原に対する免疫血清は生抗原のみと反応し,強加熱血清に対するものは加熱血清とのみ反応した.5)生肉の混合比が25%)以上の場合は本方法で検出可能であった.しかし,16%以下の場合は,検出可能な場合と不可能な場合とがあることが認められた.

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In December, 1962, tlae autlaors happened to encounter 3 swinte cases of pseudo-tuberculosis which broke otut int Tottori city. Pasteurella pseudotuberculosis was isolatedfrom the liver, spleen, lurngs, duodenum, rectum, and mesenteric lymphnodes of thesecases.The main clinical findings were dullness, inappetence, blood-stained diarrlaea, andremarkable edema in the eyelids and their neighboring areas, the mandibla, and thelower part of the abdomen.Macroscopically were observed miliary grayish-white spots in the lixer, spleen, andmesenteric lymphnodes, enteritis catarrhalis and diphtheroides, swelling and grayish-white-macular formation in the mesenteric lymphnodes, dilatation of the riglnt ventricleof the heart, remarkable general edema, moderate increase of ascites, hydropericardium, and yellowisln coloration of the general adipose tissues.Microscopically, the following findings were characteristic. Tltere were purulentand necrotic foci containing baciJIary masses and surrounded by thin granulation tissues, that is, pseudotubercules, in the lungs, liver, spleen, mesenteric lymphnodes, and Nymph-follicles of the large intestiute. On the other hand, the liver presented cellular foci, some of which contained one or a few giant cells resembling Langhanss giant cells.Both pseudotubercular and cellular foci were present in the periphery of sorne acini.Consequently, it was considered that these changes had spread via circulatiout of theportal system. In the mesenteric lymphnodes, such pseudotubercular foci as observedin the liver and spleen were few, and necrotic areas with bacillary masses were spreadmostly along the peritrabecular and subcapsular sinuses. Therefore, it was presumedthat the change had been spread to the mesenteric lymphnodes via the lymphatic stream.In the colon and caecum where tlae diphtheroid change was present, the lymphfollicleswere hyperplastic and pseudotubercular foci sometimes formed in them.It was considered that loss of the resistance caused by general steatitis autd parasi-tism of Balanti

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Chicks were given 0, 3, 9, and 27 ppm of pyrimethamine contained in a diet for12 consecutive weeks and examined both clinically and histopathologically. The healingprocess of the pyrimethamine-induced Iesion was also observed.l. Clinically, chicks exhibited remarkable hyperchromic anemia and retardedgrowth after 27 ppm administration. They showed slight anemia after 9 ppm adminis-tration, but scarcely any after 3 ppm.2. Pathologically, cl?icks showed remarkable reduction in ertyhropoiesis in bonemarrow, lymphocytic decrease in all the lymphatic tissues and swelling of reticulum cellsafter 27 ppm administration. They showed medium reduction in erythropoiesis after9 ppm, and only slight damage after 3 ppm.3. Peripheral red blood cell count and hemoglobin value were lowered after Tweeks administration of 27 ppm, but were restored approximately to the initial level inl week. Histologically, however, erythropoiesis in the bone marrow and the Iesion oflymphatic tissues were not sufficiently cured within this period of time.4. It has become clear from these results that pyrimethamine, which is usuallyemployed for the prevention of Leucocytozoon caulleryi infection in chickens, willscarcely produce any damage on chicks in a dose of 3 ppm or below, even if it is admin-istered continually over a long period.

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