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Volume 41, Issue 6
Displaying 1-15 of 15 articles from this issue
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Kohachiro KOGA, Yasushi OKAMURA, Yasumasa NOTOMI1965 Volume 41 Issue 6 Pages 677
Published: September 20, 1965
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESS3 women who suffered from consistently anovulatory cycle were treated with indirect electric stimulation of diencephalon.
Stimulation consisted of the following factors : direct current 3 mA, 3 min and 2mal per week.
By means of the electro-stimulation therapy which in this instance was used in patients with consistently anovulatory cycle, the authors were able to achieve a definite improvement in all of 3 cases.View full abstractDownload PDF (40K) -
Mitsunori MURATA1965 Volume 41 Issue 6 Pages 678-679
Published: September 20, 1965
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSThe in vitro resin uptake of radioactive corticosteroids from serum has been studied to establish a simple method for the determination of cortisol in serum (or plasma).
1) On the in vitro resin uptake of 14C-4-Cortisol (obtained from The Radiochemical Centre in England) from serum : Two ml of sample serum was pipetted to a small test tube containing a known amount of 14C-4-Cortisol. After vigorous shaking, 1 ml of it was pipetted to another test tube containing 600 mg of Amberlite IRA 400 formate. Then, it was placed in a refrigerator for 90 min at 4°C, shaking it every 10 min. The radioactivity was counted in a liquid scintillation spectrometer. The resin uptake of 14C-4-Cortisol was calculated by the follwing formula :
1-the radioactivity of the supernatant/the initial radioactivity×100.
The results indicate that the resin uptake of 14C-4-Cortisol is consistent with the change of CBG (Corticosteroids-Binding Globulin) rather than cortisol level in the sample serum.
2) On the in vitro resin uptake of 3H-Prednisolone (obtained from The Radiochemical Centre in England) from serum : The procedure was the same as that of 14C-4-Cortisol except for the use of Amberlite CG 400 Type 1 instead of Amberlite IRA 400 formate. The results indicate that in vitro resin uptake of 3H-Prednisolane well reflects the cortisol levels in sera.
3) On the method for the determination of cortisol in serum (or plasma) by using the in vitro resin uptake of 3H-Prednisolone : The standard curve was determined by adding known amounts of nonradioactive cortisol to the standard serum with the same procedure as for the determination of cortisol in the sample serum (as described below).
Two ml of 95% ethanol was added to 2 ml of sample serum (or plasma) in a small test tube, mixed by shaking and centrifuged for 10 min at 3000 rpm. Two 1 mls of supernatant were evaporated to dryness in similar test tubes with a stream of N2 gas. One ml of standard serum was added to each, and the tubes were incubated for 30 min at 4°C. Then one half ml of 3H-Prednisolone solution was added to each, and the tubes were incubated again for 30 min at 4°C. Two hundreds mg of Amberlite GC 400 Tyre 1 was added to each tube. After that, the procedure was the same as that of 14C-4-Cortisol resin uptake described above. The amount of cortisol in the supernatant was calculated from the standard curve according to the resin uptake and multiplied by 100/25.6 x 50 to express it in pg cortisol per 100 ml of serum (or plasma). The figure of 25.6 means the recovery of cortisol from the supernatant.
4) The variations of cortisol levels in sera of 4 normal subjects after the administration of ACTH were determined by the present method. On the limited evidence it would appear that a good correlation is obtained with the cortisol level expected. Further investigations of the clinical applicability of this method are in progress.View full abstractDownload PDF (140K) -
Masahiro YAMAMOTO1965 Volume 41 Issue 6 Pages 680-681
Published: September 20, 1965
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSThe present studies were undertaken to investigate the mechanism (s) by which an anabolic steroid effects the serum cholesterol level in contrast to glucocorticoid action. 2-Hydroxymethylene-17α-methyl-dihydrotestosterone (HMD) were chosen as an example of anabolic steroids and cortisone acetate as that of glucocorticoids.View full abstractDownload PDF (215K) -
Kozo YAMADA, Tadataka KOIDE, Katumi AOYAMA, Tutomu KUNO, Kazuhiro WATA ...1965 Volume 41 Issue 6 Pages 682
Published: September 20, 1965
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSThe relationship of plasma Insulin-like activity (I.L.A.) with liver damage in diabetics was studied in this report. The plasma I.L.A. was measured by in vivo-assay using hypohysectomized-adrenectomized-rats. The following results were obtained.
1) The diabetic who was diagnosed with complicated fatty liver by liver biopsy, had higher I.L.A. and the diabetic who was diagnosed complicating liver fibroses or cirrhosis, had lower I.L.A.There seems to be a disturbance of insulin utilization in the former and insulin deficiency in the latter.
2) Concerning the relation of plasma hepatic enzyme activity in diabetics, the activities of GOT, GPT, and G6Pase in diabetics having lower ILA, were lower than that in diabetics having higher I.L.A. No apparent change was observed in Alkalin phosphatase activity of both groups.
3) As for plasma I.L.A. and prognosis of liver function, liver function was improved by the treatment for Diabetes, more in cases of diabetics having high plasma I.L.A. than in cases of diabestics having low plasma I.L.A.
These findings indicate that there is an intimate relationship between plasma I.L.A. and liver damage in diabetics, and suggest that the deficiency of insulin may play directly or indirectly an important role on the complication of liver damage in diabetics.View full abstractDownload PDF (73K) -
1. Studies on the fractionation of urinary 17-ketogenic steroids (17-KGS) (using periodate oxidation) and some fundamental aspects of the Metopirone test in childrenYoshio IGARASHI1965 Volume 41 Issue 6 Pages 683-684
Published: September 20, 1965
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSA modified Few's method of the urinary 17-KGS determination, by which urinary direct 17-KGS was fractionated into 2 parts of 11-deoxy-KGS and 11-oxy-17-KGS, was applied in examining the pituitary ACTH secretion capability in children in whom adrenocortical 11-fl-hydroxylase inhibitor (Metopirone, SU-4885 CIBA) was administered. Several fundamental coditions were studied with the following results.
Metopirone capsules, 250 to 500mg a dose, were administered 6-8 times for one day to a daily dosage of 2g. in children with body weight of 20kg or less, and 3g. in those over 20kg.
1. Changes in urinary 11-deoxy and 11-oxy 17-KGS, 17-OHCS (Porter-Silber Chromogen PSC) and pregnanetriol before and after administration of Metopirone were studied ; both 11-deoxy and 11-oxy 17-KGS seemed to show changes in level of 11-desoxy-cortisol and cortisol secretion. (Fig. 2 and 3).
2. Increase in urinary 17-KGS and 17-OHCS (by Reddy, Jenkins and Torn's methods) after administration of Metopirone in 25 children of various conditions were compared. The author belives that the increase in 17-KGS excretion is a better index in evaluting the response to Metopirone than 17-OHCS which often showed a low increase and would thus give a false negative interpretation (Fig. 4, Table 1).
3. Calculating the 17-KGS increment per unit body suface after administration of Metopirone in 10 cases of normal children of 3 to 14 years of age, it was noted that no differences were seen in relation to the age, and 11-deoxy was 5.57-20.87 mg/day/m2 while the total (11-deoxy +11-oxy) was 4.49-21.44 mg/day m2 ; 11-deoxy/11-oxy ratio increased to the maximum of 3.4EL-1.10, Mean (M) -±Standard Deviation (SD). Using this level as the standard, the responses were classified as normal, hyporeactive (limited pituitary reserve, frank insufficiency) and hyper-reactive. Urinary total 17-KS was also determined at the same time ; the increase were less than 1 mg/day ? m2 in chidren under 10 years of age, and the author thinks that this level cannot be used as a criteria in evaluating the response (Fig. 5, 6 and Table 2, 3).
4. As for the Metopirone administration method, its effect by divided doses and development of side effects were studied. Doses of 250 to 500mg for 6 to 12 times for one day were suitable in children. Administration of 90 to 140 mg/day/kg body weight was necessary in order to maintain the 11-deoxy/11-oxy ratio children of various diseases over the M-1.SD of the normal children, and the admistration method which satisfies both is considered to be suitable in younger children (Fig. 7 and 8).
5. A case which showed dissociation between the elevation of 11-deoxy-17-KGS and total 17-KGS after the administration of Metopirone is desribed. Differences in the ratio of 11-deoxy/11-oxy was noted in cases which showed inhibition of response to pituitary irraadiation and thyroid treatment (Fig. 9, 10, and 11).
6. Literature on basic conditions of Metopirone test in children is reviewed on the abovementioned various points (Table 4).View full abstractDownload PDF (155K) -
Shigemi ASAZUMA1965 Volume 41 Issue 6 Pages 685-686
Published: September 20, 1965
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSUnder the influence of corticosteroids, it is well known that lymphnode falls into marked atrophy. Akasu and Tateno (1963) observed experimentaly the rat lymphnodes, pretreated with cortisone, seldom attacked with the metastasis of rat ascites hepatoma, and they assumed the possibility of arrest of cancer metastasis in man.
In previous study it has been found in rat lymphnodes that the decrease of DNA contents and the rise of the ratio of RNA to DNA contents were caused by treatment with cortisone. The purpose of the present study is to investigate the changes, induced by cortisone, which probably had prevented the lymphnodes from the metastasis of implanted ascites hepatoma, by means of determination of the nucleic acid contents in the lymphoid tissues.
Forty female rats, weighing between 80 and 120g., were divided in half. Each animal of the one half received 2.5mg. of cortisone acetate, injected subcutaneously, each day for 10 days (Treated group). Each of the other half received 0.1m1. of physiological salt solution, instead of cortisone, injected similarly (Control group).
Twenty animals in all, ten animal apiece picked out of the two groups, got ascites hepatoma AH 130 implated intraperitoneally on the seventh day after the first injection of cortisone or salt solution. Then, twenty-four hours after the final injection, all animals were killed. Twenty microcurie of 32P solution was subcutaneouly injected into each animal at six hours before the sacrifice.
After the sacrifice of the animals, thymus, lymphnodes and spleen were excised for the measurement of the nucleic acid contents and the 32P amounts incorporated into the nucleic acids. Throughout this experiment, ribonucleic acids (RNA) were determined by the color reaction of its pentose with orcine and deoxyribonucleic acids (DNA) by the reaction with diphenylamine, after the separation of RNA and DNA by the method based on Schmidt and Thannhauser procedure.
The results of the experiment are summarized as follows
(1) The implantation of ascites hepatoma carried the thymus into atrophy, in which RNA and DNA contents were decreased and the incorporation of 32P into the nucleic acids were remarkably inhibited. These changes occurred in the thymus were promoted by the simultaneous treatment with cortisone.
(2) Under the influence of the implanted tumor, untreated lymphnodes swelled in size, attended by an increase of RNA contents and a lowering of the ratio of RNA to DNA contents. In treated lymphnodes, however, these changes except for a little hypertrophy were not caused by the implantation of the tumor.
Reversely, under the influence of the implantation, the increase of DNA contents and the elevation of the ratio of RNA to DNA contents were found in treated lymphodes. This elevation of ratio, induced by cortisone, seems to have some relation to the prevention of the metastasis.
(3) The incorporation of 32P into the spleen RNA was promoted by the implantation of the tumor unrelated to the treatment. In this experiment, however, neither the treatment with cortisone nor the implantation of the tumor affected the nucleic acid contents of the spleen.View full abstractDownload PDF (153K) -
1965 Volume 41 Issue 6 Pages 687-723
Published: September 20, 1965
Released on J-STAGE: September 24, 2012
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With Special Reference to EstrogenYasushi OKAMURA, Sachiteru TANAKA1965 Volume 41 Issue 6 Pages 724-726,676
Published: September 20, 1965
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSAs the pathogenesis in the earliest stages of carcinoma of the uterine cervix, many studies have been discussed about the basal cell hyperplasia and the reserve cell hyperplasia.
The authors gained the following new concepts in the experimental carcinogenesis of cervical carcinoma in d d N strain mice :
(1) There were the prickle cell carcinoma of the uterine cervix due to the prickle cell hyperplasia.
(2) After the administration of estradiol, the prickle cell hyperplasia was found in the squamous cell areas of the uterine cervix, which was adjacent to the squamocolumnar junction.
(3) The administration of estradiol promoted the carcinogenesis in the prickle cell layer.View full abstractDownload PDF (652K) -
[in Japanese], [in Japanese], [in Japanese]1965 Volume 41 Issue 6 Pages 727-730
Published: September 20, 1965
Released on J-STAGE: September 24, 2012
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[in Japanese]1965 Volume 41 Issue 6 Pages 731-739
Published: September 20, 1965
Released on J-STAGE: September 24, 2012
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[in Japanese]1965 Volume 41 Issue 6 Pages 740-750
Published: September 20, 1965
Released on J-STAGE: September 24, 2012
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...1965 Volume 41 Issue 6 Pages 751-755
Published: September 20, 1965
Released on J-STAGE: September 24, 2012
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[in Japanese]1965 Volume 41 Issue 6 Pages 756-775
Published: September 20, 1965
Released on J-STAGE: September 24, 2012
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[in Japanese]1965 Volume 41 Issue 6 Pages 776-790
Published: September 20, 1965
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSDownload PDF (1491K) -
1965 Volume 41 Issue 6 Pages 791-795
Published: September 20, 1965
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSDownload PDF (587K)
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