A 43 year old male who was admitted to the Second Department of Internal Medicine of the Kyushu University Hospital because of general malaise and headache. Clinically he had moon-face, trucial obesity with buffalo-hump, cutaneous striae and edema, osteoporosis, polycythemia, eosinopenia, polyuria, albuminuria, glycosuria with hyperglycemia, hypokaliemia and hypocalcemia. Serum total cholesteral 316mg/dl and urinary 17-Ketosteroid excretion 35mg/24 hours.
Postmortem examination revealed adenocarcinoma of bilateral adrenal corteces with pulmonary metastasis, Crooke's change of pituitary basophil cells, atrophy of Langerhans' islets, thyroid, parathyroid and testes. There was also intercapillary glomerulosclerasis.

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We observed the extravital culture of the pulp of the rabbits suffered from alloxan diabetes and the effects on the extravital culture of the pulp of the normal rabbits by the additions of the glucose solutions and the serum of the rabbits suffered from alloxan diabetes.
1) The growth of the pulp of the rabbits suffered from alloxan diabetes is poorer than the normals.
2) The extravital culture of the pulp is controled by the addition of the serum of the rabbits suffered from alloxan diabetes.
3) When the serum of the rabbits suffered from alloxan diabetes is inactivited by the heat: filtered by the chamberland's filter L₃ and absorbed by the absorbents (caorin and blood coal powder), its controllable power is reduced.
4) But, the dealed serum with those methods has the stronger controllable power than the non-dealed normals.
5) The dealed normal serum has the almost same controllable power as the non-dealed normal serum.
6) There is no intimate relationship between the control and the glucose concentrations.

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Determination of conjugated chemocorticoids has developed and its clinical significance has been observed. It is reported in this paper the hydrolysis of conjugated formaldehydrogenic chemocorticoids in blood and urine with spleen β-glucuronidase which prepared by Fishman's method.
For urinary conjugated chemocorticoids, the method which investigated by Cohen is advisable, but the problem has been remained obscure in the enzymatic hydrolysis. We researched the chemical equilibrium and deviced the incubation method. That is to say, after extracting of free from with chloroform, β-glucuronidase was added to the remaining fluid which was brought to pH 5.0 with acetate buffer. Then 10ml. of chloroform was added and incubated 40 hrs. at 38°C. For the incubation time, the flask was shaken several times in order to extracting the hydrolyzed free steroids to chloroform, and β-glucuronidase was supplied three times. Under this condition the hydrolyzed chemocortiroids was twice of these obtained without adding chloroform as much.
For conjugated chemocorticoids in blood, Bongiovanni reported the method of estimation of conjugated 17-hydroxycorticosteroids, but the method of formaldehydogenie chemocorticoids has not been reported. The results obtained by our method conjugated formaldehydogenie chemocorticoids was about two times of free form.
By these methods, it is estimated formaldehydogenic chemocorticoids in the patient with heart failure and revealed that the conjugates was less in the patient with liver dysfunction than with normal liver.

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1. It was found that the initial hyperglycemia resulted from the intravenous administration of alloxan (150mg/kg) in the rabbit previously treated by the pineal extract was more intense than in the normal rabbit and, accordingly, the succeeding hypoglycemia was so much suppressed that no hypoglycemic shock was observed.
On the contrary, the rabbits previously treated by the pineal extract showed the final hyperglycemia 48 hours after the intravenous administration of alloxan and they suffered from more severe diabetes which was insensitive to the insulin than the control.
2. While the pineal extract showed a lowering effect of the fasting blood sugar level in the normal rabbit (3. report), such an action was not observed in the dithizone-diabetic rabbit.
3. The enhancing effect of the pineal extract of the adrenalin-hyperglycemia reported previously (3. report) was not observed in the dithizone-diabetic rabbit.

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1. The glycolytic action of blood in the rabbit was accelerated by an injection of bovine pineal extract, and such acceleration was recognized 3 hours after the injection, and it was more remarkable at a later time.
2. It was observed that the inorganic phosphate level in serum was lowered immediately after an injection of bovine pineal extract, and then elevated in a few days thereafter, while cerebral extract did not show such an action.
3. It was found that the serum inorganic phosphate level expected to be lowered by intravenous administration of glucose was markedly elevated a week after a simultaneous injection of pineal extract and glucose.

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Since the thesis of General Adaptation Syndrom was first presented by Selye, it has been noted that adrenal cortex plays one of the most important roles in the cases of stress. In order to investigate the adrenal cortical function in tuberculosis, tuberculous parients were examined by epinephrine test, eosinopenic response, uric acid creatinine ratio, urinary 17-ketosteroid, Robinson-Kepler-Power test, electrolyte studies, and insulin test. The results in their relation to the clinical condition were analysed.
Results were analysed by stochastic method.
The results were as follows:
1) Most serious cases of pulmonary tuberculosis showed poor eosinopenic response of below 50%: cases having fever, far advanced lesions throngh X-ray examination and complication of intestinal tuberculosis showed insufficient eosinopenic response.
2) Seeing the eosinopenic response in ACTH intravenous injection of 45 pulmonary tuberculous patients, these cases showed poor eosinopenic response of below 50%,: cases having fever, far advanced lesions and cavity through X-ray examination, TB bac. positiv in sputum, and abnomal blood sedimentation rate.
3) The results of uric-acid creatinine ratio were related with the clinical conditions, as well as eosinopenic response.
4) As for urinary 17-ketosteroid, the total quantity of excretion for 24 hours in serious TB did not exceed 5mg. Besides, Bolinger's“17-ketosteroid creatinine ratio”, and the quantity of increasen 17-ketosteroid were closely related with lesions throngh X-ray examination.
5) In the R-K-P test, two cases out of the 45 cases were of positiv reaction. Incidentally the two cases were severe cases.
6) Putting together the results of various on the functions of adrenocortex, and considering the relationship with the clinical condition of TB, the following conculusion is stated: In cases of severe TB, various function of adrenocortex are impaired badly, in a number of ways.
7) Electrolyte studies of 16 patients with pulmonary tuberculosis were done. As for value of blood plasma Na, Cl, and K, no remarkable results were obtained.
8) 16 patients with pulmonaty tuberculosis were submitted to insulin test. These cases showed poor eosinopenic responce of below 50%: cases having fever, far advanced lesions and cavity through X-ray examination, body-weightlos, and abnormal blood sedimentation rate.
9) From the viewpoint of a comparative study on the relationship between clinical prognosis of the pulmonary tuberculosis and the results of adrenal cortical function test, the author stated: it may be considered that the results of adrenocortical function test were closely related to the prognosis of pulmonary tuberculosis.

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