All issues
Volume 67 (1991)
- Issue 12 Pages 1295-
- Issue 11 Pages 1231-
- Issue 10 Pages 1147-
- Issue 9 Pages 14-
- Issue 8 Pages 811-
- Issue 7 Pages 755-
- Issue 6 Pages 655-
- Issue 5 Pages 587-
- Issue 4 Pages 19-
- Issue 3 Pages 175-
- Issue 2 Pages 57-
- Issue 1 Pages 1-
- Issue Supplement-3 Pa･･･
- Issue Supplement-2 Pa･･･
- Issue Supplement-1 Pa･･･
Predecessor
Volume 52, Issue 2
Displaying 1-8 of 8 articles from this issue
- |<
- <
- 1
- >
- >|
-
Shigeru AONUMA, Yasuhiro KOHAMA, Shizuo NAKAJIN, Shinji YASHIKI, Hiros ...1976 Volume 52 Issue 2 Pages 93-104
Published: February 20, 1976
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSIt had been reported by authors that salivary gland hormone, parotin, was composed with subunit (parotin-subunit) which showed molecular weight of 45,000, and that parotin-subunit had rabbit serum calcium decreasing activity and the cross reactivity with rabbit anti parotin serum. In the present report, in order to study physiological chemistry of parotin-subunit, the influence of parotin-subunit on serum Ca and 45Ca levels relating to calcium metabolism, the distribution of 131I-parotin-subunit, the effect of parotin-subunit on adenyl cyclase-cyclicAMP system, the anabolic action, C-terminal amino acid sequence and sugar component of parotin-subunit were investigated. The results are summarized as follows :
1) The decrease of rabbit serum Ca after injection of parotin-subunit was related to change of Ca in stable bone, but not to inhibition of bone resorption.
2) A high concentrated localization of radioactivity of 131I-parotin-subunit was found in liver, kidney and spleen, and as much as 60% of administrated radioactivity was localized in liver at 5 min after the injection. The retention of radioactivity was found in testis, seminal vesicle, prostate, parotid gland and submaxillary gland.
3) Cyclic AMP level increased significantly in metaphysial bone, submaxillary gland and plasma after administration of parotin-subunit but in other organs with localized much radioactivities, the level did not changed. Parotin-subunit activated adenyl cyclase of particular fraction of metaphysial bone.
4) The C-terminal amino acid of parotin-subunit was Leu, and its C-terminal amino acid sequence was -Val-Ser-Ala-Thr- Leu-OH by digestion of carboxypeptidase A.
5) Parotin-subunit included 3. 3% of sugar which consisted of amino sugar and uronic acid.View full abstractDownload PDF (1184K) -
Hajime OKUMURA, Tadamitsu SUDO, Munetoshi FUJISAWA, Shuichi NAMBA, San ...1976 Volume 52 Issue 2 Pages 105-113
Published: February 20, 1976
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSA crude hCG with an activity of about 3,000 IU per mg was purified to 10,000-15,000 IU per mg of dry weight using Amberlite CG-50 chromatography combined with DEAE-Sephadex A-50 and Sephadex G-75. The alpha and beta subunits of hCG were prepared by urea-treatment of the hormone and isolated by DEAE-Sephadex A-50 chromatography. Further purification of the subunits was achieved by gel filtration on a Sephadex G-75 column.
For radioimmunoassay hCG was iodinated by the DMSO-chloramine T method. Iodination of hCG with non-radioactive iodine revealed that the addition of DMSO to the iodination mixture seemed to reduce the iodination damage to the antigenic activity of the hormone. Non-radioactive iodine substitued hCG accomplished by the DMSO-chloramine T method showed 1. 5 times more immunoreactive in the hCG radioimmunoassay than hCG iodinated by the usual chloramine T method. The radioimmunoassay of the hCG-beta subunit developed in our laboratory was satisfactory with respect to specificity ; hLH, hFSH, hTSH and hCG-alpha subunit tested were cross-reacted very poorly in our assay system. Desialylated-hCG and subunits, whose biologic potency was almost zero, exhibited also decreased immunoreactivity, about 30% of the native hormones with grossly unimpaired parallelism in their respective homologous radioimmunoassays.
The concentrations of hCG and the subunits were determined on human sera from pregnant patients during the course of pregnancy. The hCG levels reached to the peak at the first trimester of the pregnancy, however, the hCG-beta subunits varied in their concentrations poorly throughout the pregnancy periods. The hCG-alpha levels, on the other hand, depicted two distinct peaks, at the early period and the term of pregnancy.View full abstractDownload PDF (1090K) -
ACTH Releasing Activities by Various DrugsKozo HASHIMOTO, Jiro TAKAHARA, Hidemi HOSOGI, Nobuko OFUJI, Toyoko YAS ...1976 Volume 52 Issue 2 Pages 114-124
Published: February 20, 1976
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSSeveral procedures have been reported for the assay of corticotrophine-releasing factor (CRF), each having its advantages and disadvantages. This report deals with an in vitro assay of ACTH releasing activity utilizing pituitary incubation combined with ACTH radioimmunoassay.
Rat half pituitary was preincubated in 2 ml Krebs Ringer bicarbonate buffer containing 0.2 % glucose and 0.25 % BSA (KRBG-BSA) for 1.5 hr (45 min × 2). The medium was replaced by 1 ml KRBG-BSA and incubated for 30 min. Then the medium was again replaced by 1 ml KRBG-BSA or KRBG-BSA containing test materials and incubated for another 30 min. The amount of ACTH assayed by radioimmunoassay in the 2nd 30 min incubation was compared with in the 1st 30 min incubation and expressed as percentage.
In ACTH radioimmunoassay, anti-ACTH serum was diluted to 1 : 1,500-3,000. The 125I-α 1-24ACTH-antibody system was not affected by lysine-vasopressin (LVP), argininevasopressin (AVP), rat's pituitary LH, GH and prolactin. Human 1-39ACTH was used as ACTH standard, and the dilution curve of incubation medium was paralleled with the standard curve. Repeatability of immunoassayable ACTH within-assay was 174 ± 5.0 pg/tube (CV = 2.9 %).A log dose-relationship was observed between the amounts of stalk median eminence extracts (SME ; NIAMDD) added to the incubation medium and its ACTH releasing activities. The sensitivity of this assay method was at least 0.1 SME or 10 mU of LVP and AVP.
Using this method, it found that LVP, AVP, norepinephrine (100 ng/m1-200 ng/ml) and 5-hydroxytryptophane (1 μg/ml) had ACTH releasing activities but LH-RH, TRH, glucagon, dopamine, phentolamine, propranolol, haloperidol, prostaglandin Ei and indomethacin did not affect the release of ACTH.View full abstractDownload PDF (970K) -
Hiroshi TAHIRA1976 Volume 52 Issue 2 Pages 125-137
Published: February 20, 1976
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSThe renin-angiotensin system, potassium and adrenocorticotropin (ACTH) are well known as control mechanism for aldosterone secretion. However, the precise mechanism of these factors for aldosterone secretion remain still unclear. Several interesting evidences related to the effects of Coenzyme Q on the secretion and biosynthesis of aldosterone have been demonstrated. Biochemical action of Coenzyme Q is generally accepted as a component of the electron transfer process of respiration in mitochondria. Fabre et al demonstrated that significant reduction of plasma aldosterone concentration in adrenal venous by the Coenzyme Q infusion. Weinstein et al observed that urine sodium excretion decreased after infusion of Coenzyme Q into renal artery. Kumagai et al suggested that Coenzyme Q inhibited the activity of 18-hydroxylase in the adrenal cortex.
The present study was designed to evaluate the effects of Coenzyme Q on the secretion of aldosterone.
Method : 24 cases of male mong el and beagle dogs were subdivided into 4 groups. 1st group were administered intravenous infusion of Coenzyme Q, 2nd group were oraly administered Coenzyme Q for 7 weeks, 3rd group were administered simultaneous infusion of Coenzyme Q and angiotensin II and 4th group were administered furosemide oraly under the condition of continuous Coenzyme Q administration. Then, plasma concentrations of aldosterone, 11-OHCS and angiotensin I were determined during the time course.
Results and Discussions : Plasma aldosterone concentration was significantly increased after intravenous infusion of angiotensin II and decreased 45 minutes after the beginning of infusion. However, the concentration still remained higher than control level. By the simultaneous infusion of Coenzyme Q with angiotensin II, the decreased concentration again increased significantly. It seems that above mentioned results suggest possibility that Coenzyme Q potentiate the action of angiotensin II on aldosterone secretion. By the intravenous infusion of Coenzyme Q, plasma aldosterone concentration increased significantly and concentrations of plasma 11-OHCS and angiotensin I did not affected. This result suggests that Coenzyme Q may stimulate aldosterone secretion from adrenal cortex without increase of ACTH and renin-angiotensin. 4 hours after the oral administration of Coenzyme Q, plasma aldosterone concentration was increased significantly. Na/K in 24 hours' excreta was decreased by Coenzyme Q administration. The decrease of Na/K in excreta may be reflection of the increase of aldosterone secretion. Although, plasma aldosterone concentration increased for the short duration by the Coenzyme Q, it decreased gradually and returned to the control level after 7 days under the condition of continuous oral administration. By the oral administration of furosemide under prolonged Coenzyme Q administration, plasma aldosterone concentration increased significantly and remained higher than that of control. From above results, it may be expressed that prolonged administration of Coenzyme Q did not inhibit biosynthesis of aldosterone and that Coenzyme Q may stimulate biosynthesis of aldosterone.View full abstractDownload PDF (1370K) -
Shinichi YOZAI1976 Volume 52 Issue 2 Pages 138-148
Published: February 20, 1976
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSIt is well known that majority of patients with collagen diseases are women and that collagen diseases take turn for the worse or the better when the secretion of sex hormones changes greatly at menarche, pregnancy, delivery or menopause. These facts suggest that sex hormones are involved in the pathophysiology of collagen diseases.
In the present study, the responses of plasma luteinizing hormone (LH) and follicle stimulating hormone (FSH) levels following the intravenous injection of 100 pg luteinizing hormone-releasing hormone (LH-RH) were investigated in 34 patients with systemic lupus erythematosus (SLE) and in 15 patients with rheumatoid arthritis (RA).
The results obtained were as follows :
1) The magnitude of plasma LH response to LH-RH in 29 mature female patients with SLE was significantly greater than that in normal subjects.
2) On the other hand, the magnitude of plasma FSH response to LH-RH in patients with SLE was comparable to that in control subjects.
3) In patients with RA who have normal menstrual cycles, the magnitude of increase in plasma LH and FSH levels after the injection of LH-RH was the almost same as that in normal subjects. Increased responses in plasma LH and FSH levels to LH-RH were observed in 7 patients with RA who were 3 menopausal females and 4 aged males.
These findings suggest that the secretion of LH, but not FSH, in response to LH-RH might augment in patients with SLE. On the other hand, in RA patients the function of the pituitary-gonadal axis might maintain within normal limits.
For that reason, I guess the following possibilities :
1) In patients with SLE the pathological changes of the disease reached to the ovary and ovarial function was slightly suppressed and then a hypersecretion of LH was observed, 2) the hypothalamus was attacked with the disease and then an unknown mechanism caused the hypersecretion of LH.View full abstractDownload PDF (1101K) -
Nagao HEKI, Michio NOTO, Hiroyuki HOSOJIMA, Seiji TAKAHASHI, Takeshi M ...1976 Volume 52 Issue 2 Pages 149-157
Published: February 20, 1976
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSThe first succesful analysis of iodine compounds in serum and urine by mass fragmentography using GC-MS combined system was performed. The equipment used was Shimadzu LKB 9000 GC-MS (MID-PM). The TMSi derivatives of the compounds were analyzed by GC-MS system equipped with a 3 ft × 3 mm column packed with 1 % OV-1 and the temperature was programed from 200 ° to 320°C at 10 °C/min. The mass spectrum showed molecular ions at m/e 523,649,741,867 and 993 which correspond to the TMSi derivatives of MIT, DIT, T2, T3 and T4 respectively. The base peak at m/e 218 was applied to establish the precise quantitative evaluation of T4 and related compounds by mass fragmentography. The following results were obtained :
1) The minimum detectable limits of the compounds injected into the column were ca. 10pg for MIT, DIT, 20pg for T2, 50pg for T3, and 500pg for T4 respectively.
2) The sensitivity was of the order of ng or pg which enables quantitation with 1 ml of human serum and urine sample.
3) West's method was very convenient for extracting the compounds from biological fluids, and procedure can be carried out easily in a short time. The recovery rates were ca. 16. 0% for MIT, 26. 6% for DIT, 61. 5% for T2, 71. 6% for T3, and 85. 1 % for T4 respectively.
4) The ability to simultaneously analyze various iodoaminoacids is sure to be effectively utilized in studies to elucidate the relative importance of these hormones and their metabolic changes in various physiological and pathological states of human beings.View full abstractDownload PDF (811K) -
Norimichi KONNO1976 Volume 52 Issue 2 Pages 158-168
Published: February 20, 1976
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSThe interval between the onsets of the QRS complex and of the brachial Korotokoff sounds at the diastolic pressure was termed QKd and it was already known that QKd was shortend in hyperthyroidism and prolongad in hypothyroidism distinctly.
In the present study, simultaneous measurements of QKd, serum thyroxine (T4) and triiodothyronine (T3) concentrations, free T4 index (FT4I) and free Ta index (FT3I) were undertaken and attempts were made to examine the possible correlation of QKd to these parameters of thyroid function in sera.
The QKd values of 24 euthyroid subjects had a normal range of 190 to 230 msec., 10 patients with hypertyroidism had Qkd values ranging from 145 to 180 msec. and 9 hypothyroid patients had QKd values ranging from 230 to 305 msec.
Plots of QKd agaist serum T4 and Ta concentrations denoted statistically significant inverse relation in the T4 and T3 concentrations approximately less than 20 μg/dl and 400 ng/dl respectively (r=-0.59 and -0.62 respectively) and above these concentrations of T4 and T3, QKd was nearly constant at the level of 165 msec irrepective of T4 and T3 concentrations. The statistically significant inverse relations were also observed between QKd and FT4I and FT3I in the FT4I and FTaI values less than 18 and 280 respectively (r= -0.80 and -0.74 respectively) and above these values of FT4I and FT3I, QKd was remained constant.
In 18 hyperthyroid subjects receiving antithyroid medication, QKd, FT4I and FT3I were measured. QKd values from the patients whose FT4I and/or FT3I were above normal were significantly shorter than the QKd from the patients with normal FT4I and FT3I (183 ± 9. 8 msec vs 211 ± 19 msec, p < 0. 01).
In 16 patients with primary hypothyroidism receiving L-thyroxine for replacement therapy more than 6 months of period, QKd, FT4I and FT3I were measured and TRHtest was performed. FT4I and FT3I in these patients were all in normal range and 8 out of 16 patients had normal respnse to TRH-test and others were of no response to TRH-test. No difference in QKd values was obtained between patients with normal response and with no response to TRH-test.
These results indicate that QKd is more closely related to serum free T4 and free T3 concentrations and the extent of prolongation of QKd in hypothyroidism reflects the decreased serum thyroid hormone concentrations, whereas the shortend QKd in hyperthyroidism does not parallel the increased serum thyroid hormone concentrations. QKd is useful index not only for the rapid assessment of thyroid function as proposed by previous investigaters, but also for the rapid evaluation of efficacy of antithyroid drugs administered in hyperthyroidism.View full abstractDownload PDF (1009K) -
Masanobu SATOH1976 Volume 52 Issue 2 Pages 169-171
Published: February 20, 1976
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSAn electron microscopic observation was performed on the follicular capillary of thyroid of Hashimoto's disease. Material was the thyroid obtained from a 41-years-old female showing typical features of this disease both clinically and histologically. Some remarkable findings were the presence of multi-layered basement membrane like structure, and the accumulation of dense minute granular materials concerning with long processes of lymphcyte in the widened pericapillary space. From the abovementioned findings, it was postulated that there was abnormal transport on follicular basement membrane-pericapillary space-endothelial basement membrane system.View full abstractDownload PDF (1740K)
- |<
- <
- 1
- >
- >|