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Volume 47, Issue 3
Displaying 1-4 of 4 articles from this issue
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Zuiken MATSUNO1971 Volume 47 Issue 3 Pages 165-173_13,162
Published: June 20, 1971
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSPancreatic islets of rabbits administered with glycodiazine at a dose of 150 mg/kg/ day for 2 months, 6 months and 12 months respectively, were observed by light microscope and electron microscope.
Simultaneously, blood sugar, serum IRI and extracted pancreatic insulin were also observed following the term of administration.
In light microscopy, degranulation of B cells were seen as a loss of aldehyde-thionine positivity. In electron microscopy, significant decrease of beta granules and increase of mitochondria were recognized. Golgi complexes were more extensive, showed a higher incidence of dilatation of their lammelle and a larger number of premature granules.Maximal rise in serum IRI levels was observed within 3 months and then gradual decrease continued until the 9th month. Extractable insulin from pancreas decreased to about a half of the levels prior to administration.
As the result of this observation, the author concieved that B cells were stimulated to release B granules (i.e. insulin) and accordingly forced to synthesize insulin by the administration of glycodiazine. However, the vacuolization and the appearance of lysosome like body were observed in pancreatic Bcells of rabbits administered with glycodiazine during 12 months. On the basis of this result, it was suggested that the degeneration could be caused by long-term glycodiazine administration.
Furthermore, to investigate the mechanism of hormone synthesis and release from pancreatic islet cells, normal rabbits and starved rabbits were observed electronmicroscopically. This experiment was performed, because it was thought necessary, to compare the active stage of pancreas islets effected by glycodiazine on the animals with the normal and inactive stage of islets function. The data are summarized as follows.The elaboration of secretory granules by both A cells and B cells involves the activity of the rough endoplasmic reticulum and Golgi apparatus. A granule is released by emiocytosis (i.e. merocrine type). As to the release mechanism of B granule, the possibility of a diacrine type of secretion might be considered. It is suggested that zinc might be a component of B granule and that it takes part in the mechanism of synthesis and release of insulin.View full abstractDownload PDF (17746K) -
Taro TAMADA, Yoshiji TSUKUI1971 Volume 47 Issue 3 Pages 174-180,163
Published: June 20, 1971
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSA new solid phase method of radioimmunoassay for LH has been developed utilizing easily obtainable inexpensive latex particles. Latex particles were coated with r-globulin from anti-HCG rabbit serum according to the method described previously by us (J. Clin. Endocrinol. 27 : 379, 1967) and recently some anti-HCG coated latex is available commercially. For urinary LH, the linear range of this assay method is from 3.0 to 60 IU/L (2nd IRP-HMG) using a commercially available anti-HCG coated latex (Gonavislide). This degree of sensitivity is obtained after only 20hr inculation at 20°C or 3 hr at 37°C in contrast to around a week required for the double antibody method. The effect of nonspecifiic substance in urine on LH titre was eliminated after purification of antiserum by means of absorption with peduex and separation of antibody by conjugation with polymerized HCG. The coefficient of variation is less than 10% on the linear portion of standard curve. Recovery experiment revealed that extraction of LH from urine is not necessary, as far as the method described is concerned.
Assay of LH in urine from menstruating women, prepuberal girls, postmenopausal women and normal adult males yielded the results similar to those reported in the past, thus providing additional evidence for the specificity of the method.View full abstractDownload PDF (881K) -
Clinical Studies with Radioimmunoassay of Parathyroid HormoneTakuo FUJITA, Hajime ORIMO, Masahiro OHATA, Kazutoshi OKANO1971 Volume 47 Issue 3 Pages 181-188,164
Published: June 20, 1971
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSParathyroid hormone in human blood was determined by the method of radioimmunoassay using guinea pig antibody to bovine parathyroid hormone and 125I labeled highly purified bovine parathyroid hormone, with the separation of bound and free fraction by means of adsorption to dextran coated charcoal. Parathyroid hormone in normal subjects tended to decline with advancing age in both males and females. In most of the cases of primary hyperparathyroidism, parathyroid hormone in blood was increased above the normal range, and a decrease followed removal of the adenoma. Parathyroid hormone in blood was also increased in many cases of secondary hyperparathyroidism because of chronic renal failure, 2 cases of renal tubular acidosis, and 1 case of vitamin D resistant rickets. Parathyroid hormone was undetectable in blood of patients with postaperative and idiopathic hypoparathyroidism but was elevated in 2 cases of pseudohypoparathyroidism. EDTA-induced hypocalcemia caused an increase of parathyroid hormone in blood in normal subjects of various ages with and without osteoporosis, but not in patients with postoperative and indiopathic hypoparathyroidism. Radioimmunoassay of parathyroid eormone in blood thus appears to be useful in the diagnosis of various diseases of the parathyroids, although the sensitivity of the present assay method is somewhat limited because of the difference in the immunological properties between human and bovine parathyroid hormone.View full abstractDownload PDF (918K) -
1971 Volume 47 Issue 3 Pages 189-215
Published: June 20, 1971
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSDownload PDF (4118K)
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