In the experiment the rabbits were divided into the groups which were given subcutaneous injection of 2, 4, 8, 12 mg/kg of diethyl-paranitrophenyl-thiophosphate (DPT) twice a week respectively. The morphologic changes of the central nervoussystem, especially the cerebrum of those expiring after an acute, subacute, and chronic course and those surving for a long period (120-200 days) were studied.
1) In the rupture of the blood-brain barrier, dissociation of the lamina interna and externa, porosity and dilatation of argentaffin net-work, liquefaction of argentaffin fibers, liquefaction and fragmentation of lamina externa were confirmed. However, with the prolongation of surviving period, proliferation of reticular fibers in the perivasular structure or running aadially towards the glia tissue were observed. It is inferred that this proliferation of mesenchymal element is an edematous sclerosis.
2) Edemotous porosity is frequently accompanied by insudation of high molecular protein substance (PAS positive), and coincides with the ruptured lesion of the blood-brain barrier locally. It is considered that the cause of cerebral edma is classified into the edema in disturbance of the blood-brain barrier. These lesions were often found in the internal capsule, white matter above the ventricles and in the globus pallidus, reticular nucleus of thalamus, and lower portion of thalamus.
3) Edmatous environment does not always indicate an uniform mesenchymal reaction. In addition to the time factor, it was noted that the nuclei located at the cerebral basis and internal capsule were locus of mesenchymal reaction.
4) Demyelinized lesions were confirmed in the internal capsule, corpus callosum, white matter of the mesencephalon, subcortical area above the ventricles. Morphologically, they were found in the perivascular area, especially coordinating with the rupture of the perivoscular structure. Considering the metabolic mechanism of myelin, the author should like to acknowledge the vascular factor in the present experiment.
5) The movement of the glia cells in influenced by the degree of myelin sheath destruction and time factor. In the acute stage the glia cells are chiefly of the ameboid type and oligodendrogliacytes appear. In the chronic stage participation of satellite cells is conspicuous. Anisomorphic gliosis is found in the juxavascular area and isomorphic gliosis in the distant region. As scavenger cells are not found, dysfunction of glia cells may be considered.
6) Gliosis in the gray matter is not uniform and greatly influenced by the degree of destruction, time factor and participation of myelin sheath. In the present experimet morpological changes of globus pellidus and reticular nucleus of thalamus are most conspicuous.
7) Various types of nerve cell degeneration were recognized, and some specific findings were seen in certain areas. Marked edematous environement leads to a liquefactive process of acute swelling and adds vasuolar changes. On the other hand, lack of such an environement leads to cellular atrophy and frequently accompanies calcification. In other words, degenerative changes of nerve cells is influenced by the blood-brain barrier, edematous environment and time factor. Although neuronophagia is confirmed during the process of liquefaction, it is especially conspicuous in the nuclei where myelin sheath participates.
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