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Volume 30, Issue 5
Displaying 1-6 of 6 articles from this issue
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Report 10: Enzymic Properties of Monoamine Oxidase in Human KidneyNoriyuki Shimizu1970 Volume 30 Issue 5 Pages 283-289
Published: May 28, 1970
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSMonoamine Oxidase (MAO) [EC. 1. 4. 3. 4. monoamine: O2 oxidoreductase (deaminating) ] in human kidney was partially purified using Emulgen 810 as detergent and substrate specificity, pS and pH optimum of the purified MAO were studied. MAO activity was measured by the oxgenelectrode technique using tyramine as substrate. Enzymic activity was expressed as Qo2, which is defined as the amount of oxygen consumption (μM) per minute per mg dry weight of the enzymic preparations. The following results were obtained;
1) MAO in human kindey was found mainly in mitochondrial fraction, but not in soluble fraction. These results were the same as in case of hog kindey, but the specific activity in homogenate of human kidney was lower than that of hog kidney.
2) Sixty % of the mitochondrial MAO activity in human kidney was solubilized when the mitochondria was treated with the final concentration of 1% Emulgen.
3) Thirty three % of MAO activity in homogenate was found in the solubilized MAO which was collected in 30% ammonium sulfate saturated solution.
Purity of the enzyme was approximately 4-fold than that of in homogenate.
Two hundred and twenty % of MAO activity in the solubilized fraction was found in the 15-60% ammonium sulfate saturated solution. This partially purified enzyme was relatively stable for 16 days when it was kept in freezer.
4) The partially purified MAO in human kidney possessed almost the same enzymic characters as in homogenate, such as pS and pH optimum.
5) The substrate specificity was studied comparing with the case of the homogenate and the purified MAO.
The purified MAO oxidized tyramine, dopamine, benzylamine, phenylethylamine, tryptamine and hexylamine with fairly good velocity. However, no oxidation was observed in case of adrenaline, nor-adrenaline and serotonin in contrast with the case of homogenate. The enzymic properties of purified MAO in human kindey were almost the same as that of hog kidney, rabbit, and bovine liver.
It is possible to conclude that the role and function of MAO in human tissues will be able to assume by that of experimental animals.View full abstractDownload PDF (736K) -
Masao TEI1970 Volume 30 Issue 5 Pages 290-305
Published: May 28, 1970
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSPhysiologically tissue mast cells play an important role to maintain human body well by storing and discharging histamine and serotonin. On the other hand, it has been said that tissue mast cells take part in chronic inflammatory disease. There has been many reports on the behaviors of tissue mast cells in various disorders.
The author studies on tissue mast cells of surgically biopsied lymph nodes in 871 cases of various disorders from Oct. 1957 to Dec. 1967 in Showa University Hospital. The results obtained were as follows;
1. The number of tissus mast cells in the so-called normal lymph nodes was 542.13± 220.05/cm2. Their distributions in normal lymph nodes have the tendency of relative abundance in the capsule and medullary sinus.
2. In cases of nonspecific lymphadenopathy, the number of tissue mast cells was 746.30±141.44/cm2. The histopathological appearance of the satellite lymph nodes was influenced greatly by the primary lesion. For example, there were a lot of tissue mast cells in the regional lymph nodes draining the chronic gastric ulcer.
3. In the tuberculous lympnadenitis there was relative increase of tissue mast cells more than in lymph nodes of nonspecific lymphadenitis.
4. On the number of tissue mast cells in the lymph nodes with cancer metastasis there were great difference according to the deree of metastasis. There were many mast cells in the lymph nodes with metastasis only in the perilymph nodal area. And then there were fewer tissue mast cells in lymph nodes without any metastasis, metastasis only in cortical sinus, in the medullary sinus and widely destroyed lymph nodes with cancer. Generally speaking on the primary lesions, there was constantly remarkable increase of tissue mast cells in the negional lymph nodes draining the cancer of breast.
5. In the metastatic lymph nodes, there was no deffinite findings that tissue mast cells were apt to increase around the cancerous lesion.
6. In cases of malignant lymphoma the nuber of tissue mast cells is 228.31±95.44/cm2, that is, there are great decrease of tissue mast cells in the destroyed lymph nodes with tumor cells whether they are primary or metastatic lesions.
7. Judging from the physiopharmacological study on tissue mast cells there are many report about so-called “defence capacity” of tissue mast cells. Upon this problem further investigations will be needed.View full abstractDownload PDF (2082K) -
Hiroshi Nakao1970 Volume 30 Issue 5 Pages 306-323
Published: May 28, 1970
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSIt has been shown that the effect of ceruloplasmin is a new one of conditions which accelates erythropoietic activity. It is presented data obtained by autoradiographic methods combined with tissue culture to show that erythropoietc activity induced by ceruloplasmin causes an increase of DNA synthesis by marrow cells as well as anemic or hypoxic status. But it is not clear whether this increase in DNA synthesis may associated with an increase in hemoglobin synthesis.View full abstractDownload PDF (1585K) -
Hidenosuke ARAKI, Yuhei Hujikawa, Kohei KUROSAWA, Michio MANABE, Tsuto ...1970 Volume 30 Issue 5 Pages 324-331
Published: May 28, 1970
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSAmong the 1, 540 cases of childbirth, asphxia neonatorum was found in 7.9%, and the severe cases (Apgar score 0-3) in 1.8%
The obstetric factors deeply concerned with the asphyxia neonatorum were as follows:
1. About the maternal conditions: over 30 years old primiparous.
2. Among the delivery conditions:
i) over two hours prolongation in the second stage of delivery
ii) weak labor
iii) use of the labor promoting agents
iv) Cesarian section
v) C. P. D.
vi) bruch extraction
vii) change of fetal position
viii) hardness of the soft delivery way
3. About the neonatal conditions:
i) immaturity
ii) imminent asphyxia of the fetus due to amniotic fluid turbidity or diminution of fetal heart beat
iii) low body weight fetus accompanied with functional insufficiency of the placenta
iv) convolution of the umbilical cordView full abstractDownload PDF (915K) -
Atsushi KURODA, Masayuki MORIYAMA, Mikio KANDA1970 Volume 30 Issue 5 Pages 332-337
Published: May 28, 1970
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSRecently two autopsy cases of chronic pancreatitis were experienced.
Case 1. 61yrs. female. Pathological diagnosis was chronic relapsing pancreatitis.
Case 2. 74yrs. male. Pathological diagnosis was esophageal cancer (second contractive portion, squamous cell carcinoma) and chronic pancreatitis with pancreatolithiasis.View full abstractDownload PDF (3655K) -
K. Suzuki, S. Matsuishi, Y. Nishida, M. Nyu, K. Shimizu, T. Kawada, Y. ...1970 Volume 30 Issue 5 Pages 338-340
Published: May 28, 1970
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSTwo intersting cases of spontaneous perforation of the sigmoid colon was reported.
In the first case, the perforation was observed through in the mesenterium. This is the interested case which performed the big tumor of feces.
The second case was the adenocarcinoma which performed at the anterior wall of the sigmoid.View full abstractDownload PDF (3460K)
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