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Volume 29, Issue 9
Displaying 1-6 of 6 articles from this issue
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Katsuhiko Suzuki1969 Volume 29 Issue 9 Pages 495-503
Published: September 28, 1969
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSModification of DNA synthesis has a good reason to be expected the enhancement of radiosensitivity. Studies on the influences of aminopterin in radiosensitivity were carried out in yeast cells.
1) . Radiosensitivity of the cells depended markedly on the growing phases of the cells.
2) . Lethal effect of aminopterin treatments suggested“thmidineless death”in the experiment.
3) . Addition of TMP in the culture media following irradiation did not decrease lethality, however addition of IMP and several amino-acids together with TMP, which were considered to be inhibited by aminopterin, decreased the lethality.
4) . Some apparent discrepancies in the studies were elucidated by 2-14C-thymidine labeling experiments. In which, aminopterin treatment produced synchronization in the cell groups ; G-1 arrest in the cells harvested from log phase culture and G-2 from stationary phase culture were observed.
5) . From these experiments, some of them apparently conflicted each other, in was concluded that the cells were most radiosensitive when irradiated in late G-1 and early S. And aminopterin did not seem to enhance the radiosensitivity by 10-4mol. other than additive effect. These results suggested the utility of aminopterin combined with irradiation when adequately treated.View full abstractDownload PDF (913K) -
(SITES OF THE INHIBITION ARE TMP SYNTHESIS OR dCMP SYNTHESIS)Hirotsugu Munechika1969 Volume 29 Issue 9 Pages 504-517
Published: September 28, 1969
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSExperimental studies on the dependence of radiosensitivity in relation to the growing phase, to the DNA metabolism inhibition, and to labeled TdR uptake in respect to DNA synthesis were performed in yeast cells. The sites of the inhibition were TMP synthesis or dCMP synthesis. Following results were obtained :
1) . In the inocorporation of labeled TdR into the cell, the time of its high rate was agree with the time of radiosensitive. And it was suggested that the initial indution of enzymes involved in TMP synthesis was the key to the radiosensitivity.
2) . The incorporation of labeled TdR into the cells was increased by irradiation. These results might show that there had been some questions in labeled TdR incorporation being used as adequate indicator of DNA synthesis. However, the incorporation was much less in yeast cells than in E. coli or in mammalian cells.
3) . Enhancement of irradiation with 5-FU was remarkable but not with BUdR in yeast cells.
4) . As administration of TMP, dUMP, AdR, GdR, and hydroxyurea in high concentration were concerned, these substances caused cell growth inhibition and their inhitions were slightly prevented by addition of dCMP in the medium. Among them, hydroxyurea inhibiton was prevented, though slightly, not only be addition of dCMP but also by addition of AdR or GdR. From these results, it was suggested that these substances were the inhibitors in the deoxyribosidation step from CDP to dCDP. These inhibitions did not change the radidosensi-tivity, excepting hydroxyurea, which enhanced it in pretreatment.View full abstractDownload PDF (1550K) -
Yasuo Naito1969 Volume 29 Issue 9 Pages 518-524
Published: September 28, 1969
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSBone marrow cells were counted and uptake of labeled IUdR in the cells were measured several days following total body irradiation in mice.
And surviving fractions were scored 30 days after the second dose given 0 to 7 days following first dose.
These procedures made it possible to know the recovery process at the time of the seond dose.
Time interval of one or two days did not reveal any split dose effect, however three days interval proved remarkable effect of split dose.
Assuming that the rate of surviving fraction of bone marrow cells was equal by first dose as well as as by second dose, it was able to contrast the surviving fraction of the mice to the surviving fraction of the marrow cells. Number of bone marrow cells at the time of second dose was that which survived the first dose and proliferated by the time of second dose.
It was likely that so called bone marrow death was directly correlated to the number of the bone marrow cells survived and that recovery dependent dose fractionation effect in mice lethality was also directly related to proliferation of survived bone marrow cells.View full abstractDownload PDF (672K) -
Mitsuo Odagiri, Sukehide Itoh, Haruo Arai, Koh Gotoh, Masao Takasugi, ...1969 Volume 29 Issue 9 Pages 525-530
Published: September 28, 1969
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSThe 78 years old male patient was treated under the diagnosis of hypertension and atrioventricular block. He became suddenly unconcious and expired 25 hours after the episode with all energetic medical care. The autopsy disclosed widespread sarcoidosis including cardiac involvement. The lungs, liver, cervical muscles, lymph nodes were also involved.View full abstractDownload PDF (6473K) -
Jui-Yen Tseng, Keizo Takahashi, Shiro Matsuzaki, Hiroshi Maeda, Ikuo M ...1969 Volume 29 Issue 9 Pages 531-533
Published: September 28, 1969
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSDiazepam was administered intravenously for induction in 30 cases of general anesthesia, and for sedation in 5 cases of spinal anesthesia and 5 cases of epidural anesthesia.
The following results were obtained.
1. 10 mg of Diazepam was given by intravenous injection for induction of general anesthesia. Satisfactory hypnosis and sedation developed after one minite.
2. The sedative effects of Diazepam were satisfactory in the patients in whom the effects of spinal or epidural anesthesia were good. However, in those patients in whom the effects of regional anesthesia were not enough, on effect or untoward effects such as excitement were observed.
3. Circulatory and respiratory system were not affected significantly by diazepam.
4. The amnesic effects of diazepam were marked.View full abstractDownload PDF (316K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...1969 Volume 29 Issue 9 Pages 534-541
Published: September 28, 1969
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSDownload PDF (997K)
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