Stated in the following are the findings of the varying stainings and the examinations of PAS and metachromasia applied on the collagenous, argentaffine and elastic fibers of the skin of 30 human fetuses during the period from the 2nd to the 9 th month of embryonation
1. Argentaffine fibers appear in the epidermo-dermal junction as early as in the 2nd month of embryonation. This author wishes to support the opinion to say that the generation of argentaffine fibers is influenced by the contact of the ectoderm and the mesenchymal tissue. The reticular fine argentaffine fibers which appear in the upper part of the dermal papillae, however, are considered to develop by different mechanisms.
2. Prior to the appearance of fibrous element in the dermis, PAS reaction positive granules appear in mesenchymal cells and the granules diminish in number following the appearance of fibers, in the dermis suggesting that mesenchymal cells are playing an important role in the fiber formation of human skin.
3. Findings of the present study confirmed the opinion to say that collagenous fibers are metamorphosed from the argentaffine fibers appearing in the lower layer of the dermis.
4. Elastic fibers begin to appear from the 7th month of embryonation.
5. Metachromasy was found to appear in the upper layer of the dermis from the 5th month of embryonation.

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Presently, the pathogenesis of nephritis is considered to be the allergic changes produced by antigen-antibody reaction. Various kinds of nephritis have been reported up to present such as Masugi's nephritis, γ-globulin nephritis, the nephritis caused by repeated administration of hemolytic streptococcus etc.
Based on the findings that fibrinolysis appears in the blood of the patients of both acute and chronic nephritis, an investigation was made, whether nephritis does develop in the animals administered of the fibrinolysin or not.
After injection of this substance into the renal artery of rabbits, Fujisawo and Tsuruta recognized the development of nephritis by the use of blue globulin substance, which had a strong filrinolytic activity, while white globulin substance which had no activity gave no findings suggestive of the development of nephritis. Since this globulin substance isolated from canine plasma is a heterogeneous protein to rabbits, similar experiments were repeated on the male dog from which fibninolysine was obtained. Results obtained were as follows :
1. Blue globulin substance which possessed a strong fibrinolytic activity (potency 32 units) was isolated from a dog and injected into the renal artery of the same dog. Clinically, findings suggestive of nephritis such as the appearance of protein, erythrocyte, leucocyte, cast, renal epithelium were recognized in the urine. Histopathologically, findings suggestive of acute or subacute diffusive glomerulitis such as the swelling of the glomerulus, adhesion, collapse, destruction, colliquation, nuclear increase, appearance of hyaline substance, and appearance of protein-like substance in Bowman's capsule and in renal tubule were recognized.
In the dogs administered of blue globulin substance, moreover, there was a general tendency to show the decrease in 17 ketosteroid, chemocorticoid, blood and urinary vitamin C contents and urinary Na and K contents. Blood Na and K level showed, however, no remarkable changes.
2. On the other hand, when white globulin substance (potency below 4 units) without having fibrolytic activity was administered in the same manner on the dog from which it was isolated, the urine was free from protein, erythrocyte, casts and there was no findings suggestive of nephritis clinically as well as histopathologically.
3. Repeated administration of vitamin C prior to the injection of blue globulin substance inhibited the development of nephritis.
4. Cortisone treatment in a dose of 10 mg/kg before the administration of blue globulin substance, however, failed to prevent the development of nephritis showing the appearance of protein in the urine

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Stated in the following are the findings of the in vivo distribution of theophylline (TP) and theobromine (TB) in the chick embryos after an injection of 1.0 mg or 2.0 mg of the former or 2.0 mg of the latter into the developing eggs.
Allantoic fluid showed an evident increase following the administration of TP, while it shhwed a decrease in the case of TB.
Both substances are recovered in a high concentration from the allantoic fluid as well as from the amniotic fluid. The total quantity of TP and TB in the allantoic fluid, however, was always larger than that of the amniotic fluid.
After administration, both substances are distributed in the brain, liver, heart and kidney in a relatively high concentration, especially, TB concentration in the kidney is remarkably high.
Both TP and TB are easily excreted into the allantoic fluid.

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As the result of animal experiments, it was clarified that mesotartaric acid has some antiglycosuria activity and that sodium mesotartrate does not have this activity.

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1. No significant difference was recognized in the serum Ca content between normal people and the patients of alopecia circumscripta and disseminata.
2. Serum dialyzable Ca content of the patients of alopecia circumscripta and disseminata was lower than that of the normal people.
3. Serum total Mg content and dialyzable Mg content of alopecia patients were higher than the respective finding of the normal people.
4. No significant difference was recognized in the serum inorganic phosphate content between normal people and the patients of alopecia circumscripta and disseminata.

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Stated in the following are the findings of general pharmacological activities of β-mercapto-ethylamine·HCl (MET), S, β-aminoethylisothiuronium. Br·HBr (AET), reduced glutathione (GSH) and l-cysteine (CySH) .
On subcutaneous injection, the LD₅₀ of MEA was found to be 274.47 mg/kg. and that of AET was 647.9 mg/kg, the former showing higher toxicity than the latter. The toxicity of MEA and AET was evidently higher than that of GSH and CySH.
MEA, CySH and GSH cause the elevation of the blood pressure and the dilatation of the coronary vessel of rabbit, while AET shows no such effects.
MEA and AET show similar pharmacological activities, namely, temporary inhibition of the respiration and the heart pulse in situ of rabbit by an intravenous injection in a dose of 5-10 mg/ kg, and the enhancement of the motility of isolated intestine. They show the contraction of the ear vessel of rabbit and, when a large dose was administered, they enhance the contraction of the isolated heart of toad.
Pharmacological activities of CySH and GSH are mostly the same. Namely, they elevate the blood pressure, accelerate respiration and dilate the coronary vessel of rabbit. In a lower concentration, they cause the contraction of the ear vessel, while they cause its dilatation when the concentration is high. They, moreover, show remarkable inhibition of the motility of isolated intestine in a concentration of 10^-3 though such influence is absent in a concentration of 10^-4.

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[in Japanese]

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Tendency of the motility is the same in either case of nicotine loading made on fertilized eggs before incubation or on the 5th day after starting incubation. Appearance of deformity is recognized on the eggs loaded with nicotine more than 1.0 mg. When viewed from the stand-point of the amount of deprotenized nitrogen in allantoic fluid, the influence of nicotine loading can evidently be recognized from 1/10 of the above mentioned dosage influencing the development of chick embryos.
Appearance of nicotine hydrops is not recognized on the eggs of the 9th day incubation, but it appears most remarkably on the eggs of the 12th day incubation, gradually decreasing thereafter.

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1. γ-aminocrotonic acid injected intravenously into rabbits is immediately excreted into the urine from the blood. More than 60% of the injected acid, however, is not demonstrable. In addition, an increase in the amount of glycine which is considered to be the decomposition product of this acid by oxidation is not recognized.
2. Upon loading embryonating eggs with γ-aminocrotonic acid, the amount of glycine excretion into the allantoic fluid is increased.
3. Loading of γ-aminocrotonic acid gives an evident disturbance on the liver of chick embryos.

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A curve for the determination of Caeruloplasmin value was first prepared by the use of crystalline caeruloplasmin and its value was determined on the sera of various diseases.
1. Serum caeruloplasmin values of normal healthy individuals were 15-19 mg/dl.
2. Patients of anemia, malignant tumor, infectious diseases, rheumatic arthritis, pleurisy and pregnant women showed higher levels of serum caeruloplasmin, while those of Wilson's disease and chronic nephritis without accompanying infections and anemia showed lower levels.
3. Caeruloplasmin is said to occupy 90-95% of serum copper, but anemic serum did not show a definite ratio of serum caeruloplasmin and serum copper. In the majority of such cases, serum copper level is high in relation to the respective caeruloplasmin level.

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[in Japanese]

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Studies were made on the daily rate curves of the intraocular pressure drawn for 18 normal rabbits. White rabbits weighing 2-2.5 kg were used and daily rate curves were drawn by the measurement of intraocular pressure 8 times per day at 3, 6, 9, 12 O'clock in the afternoon and at 3, 6, 9, 12 O'clock in the morning.
As the result of above studies, it was concluded that the daily rate of the changes observed on the intraocular pressure of normal rabbits lies within the range of 3-6 mm Hg.
As for the type of intraocular pressure curve, 6 eyes were bimodal, 10 eyes ascending, 7 eyes were descending and 10 eyes were constant. Namely, four different types were recognized on the intraocular pressure curves of normal rabbits, but the frequency of their appearance was not in agreement with the findings of human eyes reported by Langley et al.

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