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Volume 60 (2000)
- Issue 6 Pages 657-
- Issue 5 Pages 553-
- Issue 4 Pages 423-
- Issue 3 Pages 309-
- Issue 2 Pages 147-
- Issue 1 Pages 1-
Volume 60, Issue 4
Displaying 1-18 of 18 articles from this issue
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[in Japanese]2000 Volume 60 Issue 4 Pages 423
Published: August 28, 2000
Released on J-STAGE: September 09, 2010
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[in Japanese]2000 Volume 60 Issue 4 Pages 424-434
Published: August 28, 2000
Released on J-STAGE: September 09, 2010
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[in Japanese]2000 Volume 60 Issue 4 Pages 435-442
Published: August 28, 2000
Released on J-STAGE: September 09, 2010
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[in Japanese]2000 Volume 60 Issue 4 Pages 443-447
Published: August 28, 2000
Released on J-STAGE: September 09, 2010
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Tadashige MATSUMOTO2000 Volume 60 Issue 4 Pages 448-453
Published: August 28, 2000
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSMacroscopic examinations of morphology and attachment of the anterior part of the lateral meniscus were made on 128 knees from 64 cadavers (average age 67.9 years) . On the anterior part of the lateral meniscus, three fiber bundles were identified. Each bundle was a devious fiber bundle (a) to the transverse ligament connected to the medical meniscus, a fiber bundle (b) which transforms to the anterior cruciate ligament (ACL), and a fiber bundle (c) attached to the lateral intercondylar eminence of the tibia from behind the ACL. Bundle (a) was extremly thin, and is not considered to have a significant role in dynamically anchoring the anterior portion of the lateral meniscus. Bundles (b) and (c) were of a uniform thickness. There were 4 types of combinations of these three bundles, and all knees could be classified into one of these 4 types; Type I (38 knees, 29.7%) With three bundles (a, b and c) ; Type II (73 knees, 57.0%) the largest category, 2 bundles (b and c) ; Type III (9 knees, 7.0%) two bundles (a and c) ; Type IV (8 knees, 6.3%) a single bundle (c) . There were no cases observed of any other possible combinations such as (a) + (b), (a) only, or (b) only. Our results suggest that a method to avoid injury to these attachments during a reconstruction procedure is to perform a precise arthroscopic analysis at first to verify the fiber bundle that serves as the attachment for the lateral meniscus anterior section then purify the remnant ACL, or preserve the lateral portion of the remnant.View full abstractDownload PDF (5098K) -
—Analysis on Stages of Crisis after Breast Cancer Surgery—Fumiyo FUJINO, Yoshiharu HOSHIYAMA, Takeshi KAWAGUCHI2000 Volume 60 Issue 4 Pages 454-461
Published: August 28, 2000
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSThe authors performed a mail questionnaire on 96 out of 105 breast cancer patients one week after breast cancer mastectomy. All paticipants gave informed consent for participation. The questionnaire was composed of 16 items based on Fink's crisis model, support questionnaire, commercial TEG questionnaire for ego-state and self-esteem questionnaire (10 items of Rosenberg translated by Kan) . The following results were obtained. In terms of TEG scores, CP (Critical Parent) scores and AC (Adapted Child) scores tended to be lower as patients proceeded from the defensive retreat stage to the adaptation stage, while NP (Nurturing Parent) scores, A (Adult) scores and FC (Free Child) scores tended to become higher as they moved from the defensive retreat stage to the acknowledge and adaptation stage. FC scores of the patients in the adaptation stage were significantly higher (p<0.05) than those in the defensive retreat stage. The FC/AC ratio was significantly higher (p<0.05) as the patients moved from the defensive retreat stage to the acknowledge and then to the adaptation stage. The average self-esteem score was 29.6 and increased as the patients improved from the defensive retreat stage to the acknowledge and adaptation stage. This indicates that self-esteem affects the development of crisis, and patients with low SE need individualized care.View full abstractDownload PDF (853K) -
Katsutoshi MOTOHASHI, Tamio HAGIWARA, Tsunamasa WATANABE, Satoko YAMAS ...2000 Volume 60 Issue 4 Pages 462-470
Published: August 28, 2000
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSPutative HLA class II associated protein I (PHAPI) also known as pp32, LANP and APRIL is an acidic nuclear protein of molecular mass 32-35 kDa. PHAPI proteins and cDNAs are found from various tissues and cells of mammals; however, reported primary structures of PHAPI from different tissues are not identical although they are obtained from the same speces. To investigate the molecular diversity of PHAPI, we cloned the cDNAs encoding the PHAPI from mouse embryo cDNA libraries. Over 20 kinds of cDNA sequences were isolated from 7-17 day mouse embryo cDNA libraries. These cDNA sequences were divived into two types on the basis of the nucleotide sequences and the predicted amino acid sequences and they encoded PHAPI isoforms which deleted a various coding region such as the nuclear transport signal and phosphorylation site by the PHAPI kinase. Of these isoforms, one group was already expressed in a 7-day embryo and constitutively expressed at various stages of embryo development and in the various tissues of an adult mouse. Other groups of PHAPI isoforms were detected from an 11-day mouse embryo. Expressed isoforms of this group were different at the stage of embryo development and in the tissues, suggesting that each isotype of this group has not only structural differences but also functional differences. Although the molecular mechanisms of these PHAPI isoforms are still to be clarified, they might have important roles in the development of the embryo and physiological functions of tissues.View full abstractDownload PDF (3379K) -
Koki UMEZAWA2000 Volume 60 Issue 4 Pages 471-486
Published: August 28, 2000
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSThis study was performed in 33 ski jumpers taking part in the All Japan Ski Competion to analyze their “Satz” motions during the contest with a three-dimentional computer motion analyzing system (APAS) . These jumpers were divided into three groups: the Junior, Special, and Nordic combined jump groups. The take-off motion was measured in terms of angular velocities of three joints of the lower extremity and approach speed at “Kante”. The peak angular velocity of the knee joint was synchronized with that of the hip joint only in the Special and Nordic combined jump groups. Slight ankle dorsi-flexion was included in the “Satz” motion. The peaks of angular velocities in the hip and knee joints were higher in the Special jump group than in the Nordic combined jump group. In the Junior jump group, the hip and knee joints depicted various curves, In the motion of “Satz”, 1) the peak angular velocity of the hip joint was synchronized with that of the knee joint, 2) statistically, the greater the angular velocities of the hip and knee joints, the longer the jump, 3) the jumpers used joints of the ankle not to kick the “Kante”, but push it, 4) there was not any statistically significant difference in angular velocity of the joint among the three jump groups, and 5) statistically the greater the approach speeds, the longer the jump. In order for the motion of “Satz” to overcome vertical drag and air resistance, the power of the muscles of the back, low back, buttocks, and lower extremities are also essential.View full abstractDownload PDF (2459K) -
Eigo SASAKI, Yasuyoshi TOSA, Yoshiaki HOSAKA, Taku TSUKAGOSHI2000 Volume 60 Issue 4 Pages 487-492
Published: August 28, 2000
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSThis study was undertaken to evaluate the application of a fibrocollagenous tube as a nerve conduit on 12 Japanese white rabbits. The fascia measuring 20×30 mm was harvested from the dorsal fascia and carefully wrapped around a silicon rod 3.0 mm in diameter. Subsequently, the fascia-wrapped silicon rod was implanted into the subcutaneous pocket on the thigh. Two weeks later, the fascia-wrapped silicon rod was taken from the subcutaneous pocket. The silicon rod was removed from the material and the fascia-wrapped f ibrocollagenous tube was crosslinked by flushing with glutaraldehyde solution. Subsequently, the 3 × 10 mm fascia-wrapped tube was prepared as a nerve conduit. Using the microvasucular technique, the tube was interposed into the divided sural nerve. As the control, a 3 × 10 mm silicon tube was interporsed into the rural nerve in the other side. Three, six, and nine weeks later, regenerated nerves were observed by the light microscope. The result was that the number of regenerated nerves of the fascia-wrapped fibrocollagenous tube was almost the same as that of the silicon tube. However, it has been suggested that laminin can induce nerve regeneration, and laminin exists in the fascia. Therefore, to confirm the existence of laminin in the tube, we performed anti-laminin and anti-melosin stains. The result was that laminin and melosin existed in the tube. It was concluded that the fascia-wrapped f ibrocollagenous tube may be used clinically in the future as a nerve conduit after some improvements.View full abstractDownload PDF (4587K) -
Kazuhiko YOKOYAMA, Masaaki NAGATSUKA, Tsuyoshi OKUDA, Tetsuo SHIBATA, ...2000 Volume 60 Issue 4 Pages 493-500
Published: August 28, 2000
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSFourteen patients with advanced cervical cancer (FIGO stage II b ; 2, III b ; 10 and N a ; 2) received neoadjuvant chemotherapy (NAC) with intra-arterial infusion. The patients underwent radiation therapy (FIGO stage III b, n=17) and the survival rate was compared. Patients were treated with consecutive infusion of CDDP (10mg/day) and continuous infusion of 5-fluorouracil (250 mg/day) from bilateral intra iliac arteries in each course for 10 days. Complete response and partial response was achieved in 3 and 9 patients, respectively, and the total response rate reached 85.7%. Eleven patients received radical hysterectomy after NAC. NAC was effective for pelvic lymph node metastasis in all six patients. However it was not effective for para-aortic lymph nodes metastasis. Among the patients with stage III b, an 80% 3-year survival rate and a 60% 4-year survival rate were obtained. Significantly higher survival rates of NAC in 3- and 4-year olds were found in the patients who received NAC and not of the patients who received radiotherapy. Lung, bone and para-aortic lymph node metastasis was found in 3 out of 4 patients. However, 7 out of 10 patients in the radiation group alone were found to have intra-pelvic recurrence. No obvious side effect was found during NAC. NAC with intra-arterial infusion chemotherapy is effective for advanced cervical cancer. From the present study, it is suggested that additional intra-venous chemotherapy and/or radiation therapy for extra-pelvic metastasis is required to improve the survival rate for advanced cervical cancer.View full abstractDownload PDF (791K) -
Yasuyuki OKAMURA, Hisato IKEDA, Kiyoshi MATSUMOTO, Tetsuhiko TACHIKAWA2000 Volume 60 Issue 4 Pages 501-512
Published: August 28, 2000
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSAmong malignant tumors, glioblastoma is particularly known for its poor prognosis, with rapid cellular proliferation and infiltration, which progresses to obliterate the surrounding cerebral tissue. For this process of tissue destruction, proteases that are secreted by the tumor cells play a major role. Among these proteases, matrix metalloproeinases, cysteine proteinases (CP), and serine proteinases are representative enzymes responsible for such actions. For the process of tumor infiltration in glioblastoma, a specific CP has been recognized from the substrate specificity of the cerebral tissue. This CP is normally under the control of its inhibitor and a loss of its balance is the hallmark of malignant tumor cells. In glioblastoma cells, the expression of CP and malignancy are positively correlated. The present study intends to delineate the action of the inhibitor of CP on glioblastoma and explain the action mechanism involved in this process. For the glioblastoma cell strain of the study, T98G and human glioma derived glioblastoma cells (SRG4-1) were used. These cells were raised under prescribed culture conditions and treated with a CP inhibitor (CPI), ALLM, at 3 concentrations (0, 0.5, 1.0μM/ml) to observe its effects on cell proliferation suppression, cell cycle, and apoptosis. It was found that the inhibitory effect of ALLM on cell proliferation of glioblastoma varies depending on the concentration of the inhibitor. When changes in the cell cycles associated with the process of cell proliferation were examined, G2/M arrest was recognized. Although no ladder pattern was recognized in DNA fragmentation, electron microscopic examination revealed nuclear atrophy and aggregation of nuclear chromatin. Furthermore, caspase-3 activities were found to have increased significantly. In the immunohistochemical analyses, the Tunel method and annexin V method procedure suggested the existence of apoptosis. Involvement of apoptosis was suspected in the action of ALLM to inhibit the proliferation of glioblastoma cells.View full abstractDownload PDF (6717K) -
Nobuyasu MATSUDA, Yasutada ONODERA, Takao IKEUCHI, Yoshio KAI2000 Volume 60 Issue 4 Pages 513-517
Published: August 28, 2000
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSIt is recognized that well-differentiated adenocarcinoma of the prostate responds well to anti-androgen therapy. However, well-differentiated adenocarcinoma is sometimes hormone independent and poorly differentiated adnocarcinoma sometimes has good response to hormone therapy. Therefore, cell-cycle analysis of fresh tissue of prostate cancer using flow cytometry was assessed as a possible prognostic factor for prostate cancer. Cell cycle analysis was performed for 35 cases with the prostate cancer to be compared with pathological grades, clinical stages and patients' ages. The results were as follows : 1) The ratio of G0/G1 status of prostate cancer was smaller than that of the benign prostatic hypertrophy, and the ratio of G2/M was greater in reverse. 2) The DNA histogram patterns showed a diploid pattern in 21 cases (60.0%), aneuploid pattern in 10 cases (28.6%) and tetraploid pattern in 4 cases (11.4%) . All cases of the benign prostatic hypertrophy and the well-differentiated adenocarcinoma showed diploid patterns. All cases of the tetraploid pattern were poorly differentiated adnocarcinomas. The most severe cases of adenocarcinoma experienced the greatest number of occurrence of aneuploid DNA values. 3) Patients with a DNA index 1.0 were older than those with a DNA index over 1.0. It was suggested that the cell cycle of prostate cancer was different from that of benign prostatic hypertrophy. The DNA histogram of prostate cancer correlated well with the pathological grade, and the DNA index seemed to correlate with the period of initial clinical cancer. These results suggest that the determination of cell cycle analysis could be used for diagnosis of prostate cancer, especially for cell grading.View full abstractDownload PDF (509K) -
Tsuguo FUJIHARA, Masaaki NAGATSUKA, Norio SUZUKI, Nahoko SHIRATO, Taro ...2000 Volume 60 Issue 4 Pages 518-523
Published: August 28, 2000
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSInsulin-like growth f actor-I (IGF-I) plays an important role in the regulation of growth during puberty. Although IGF-I levels are known to be related to sex steroids, sex difference related to the effect of sex steroids on IGF-I is still unknown. To investigate the effect of sex steroids on IGF-I production in both sexes of immature rats, the following study was undertaken. Immature female rats (Wistar) were ovariectomized (OVX, n= 20) and male rats were orchitectomized (ORX, n=20) 14 days after birth. Estradiol (E2, 0.01 nmol/body, n=5), testosterone (T, 1 nmol/body, n=5) and dihydrotestosterone (DHT, 0.1 nmol/body, n=5) were administered daily for 4 weeks. A sham operation (n=5) was also performed as the control. On day 48, rats were sacrificed and blood samples and liver tissues were obtained. Serum IGF-I concentrations were measured by the RIA method. Northern blot analysis was used to study the IGF-I mRNA expression in the liver. Body weights were increased by OVX, while no change by ORX was observed when compared with the sham group. Estrogen administration to OVX/ORX decreased body weight. Administration of androgen increased the weight in both sexes. Serum IGF-I levels and IGF-I mRNA expression in the liver were increased by OVX, but there were no changes by ORX. Serum IGF-I levels and IGF-I mRNA expression in livers were decreased in OVX rats but were increased in ORX rats by estrogen administration. On the other hand, administration of androgen to OVX/ORX rats increased serum IGF-I levels and IGF-I mRNA expression in livers. From the results obtained above, it was demonstrated for the first time that estrogen has different effects on IGF-I production between male and female immature rats although androgen stimulates IGF-I production in both sexes.View full abstractDownload PDF (1471K) -
Fumiya OHKURA, Hiroshi FUJIKAWA, Hiroshi SAITO, Takumi YANAIHARA2000 Volume 60 Issue 4 Pages 524-531
Published: August 28, 2000
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSSince estrogen and androgen receptors are found in osteoblastic cells, a direct effect of sex steroids on cell growth and proliferation in osteoblasts is suggested. Although the existence of sex steroid converting enzymes in osteoblastic cells have been reported, estrogen and androgen function on osteoblasts is not clear yet. To determine androgen metabolism in osteoblastic cells, the following in vitro incubation study was carried out. Cell free homogenate of a rat osteoblast cell line, UMR106-01 and C26, were incubated with [4-14C] -Testosterone (T), [4-14C] -Androstenedione (A) . In UMR106-01, formation of dihydrotestosrerone (DHT), 5α -and rostanedione (5α-A) from the corresponding substrate was identified by crystallization to constant specific activity. Michaelis constant (Km) for T was estimated as 42μM. In C26, T was identified from A, and Km was estimated as 41μM. The expressions of 5α -reductase type I messenger ribonucleic acid (mRNA) in UMR106-01 and 17 β-Hydroxysteroid dehydrogenase (17 β-HSD) type I in C26 were demonstrated by a reverse transcription-polymerase chain reaction. Formation of DHT from T and 5α -androstanedione from A and were found in UMR106-01, and T formation from A was found in C26. These results demonstrating the enzyme activity of 5α -reductase and 17 β -HSD suggested the capacity of osteoblasts to convert androgen to active forms. In addition, these results indicate that local metabolism of androgens in osteoblastic cells may play an important role on bone metabolism.View full abstractDownload PDF (2012K) -
Masahiro SEKIYA, Hiroshi CHIBA, Masaaki NAGATSUKA, Kiyofumi KAWAI, Tak ...2000 Volume 60 Issue 4 Pages 532-539
Published: August 28, 2000
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSPlasma levels of dehydroepiandrosterone (DHA), dehydroepiandrosterone sulfate (DHA-S) androstenedione (Δ 4A), androsterone (5 α -A) and androstanediol (5 α -Ad) levels were measured by specific radioimmunoassay in pubertal girls to elucidate the physiological role of these hormones on female sexual development. One hundred twenty girls between ages 6 and 17 were selected for this study. Blood samples were collected into tubes containing 1W/V % EDTA and immediately centrifuged. Steroids in plasma were extracted and separated with a Sephadex LH-20 column. RIA was applied using a specific antibody for each steroid. Plasma DHA levels increased from age 8 followed by a significant increase between ages 10 and 11. DHA-S levels in plasma started to increase earlier than DHA. Plasma Δ 4A levels before menarche showed no significant change until age 10, and gradually increased after menarche. Plasma 5 α -A levels started to increase from age 9 and reached maximum levels at age 17. On the other hand, plasma 5 α -Ad levels before menarche were higher than after menarche. There was a significant decrease in 5 α -Ad levels between ages 11 and 12. Changes of plasma androgen levels in puberty were studied. This is the first report demonstrating the changes of 5 α -Ad levels in pubertal girls. A significant decrease of 5 α -Ad suggested that this steroid plays an important role in the initiation of menarche.View full abstractDownload PDF (749K) -
Tadashige MATSUMOTO2000 Volume 60 Issue 4 Pages 540-543
Published: August 28, 2000
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSWe treated 2 young case of lumbar spondilolysis. These cases were diagnotice by X-ray, CT, MRI and bone scintigraphy. The first case was 14-year old. This case was none-union type, so spondylolysis coud not treated by casting or brase. The second case was 16-yea old. Bone scintigraphy suggest that casting methord was of f ecitve. 6weeks of cast was done. And after that we planning 3 months of brace. L4 spondylolysis was recoverd. But L5 spondylolysis was not treated well. Two types of spondylolysis was needed to treated other methord. Bone scintigraphy is very usef ull to diagnosis two types.View full abstractDownload PDF (1784K) -
Masahiko YAMAGUCHI, Masaoh KUZUME, Tadashi MATSUMOTO, Akihiko MATSUMIY ...2000 Volume 60 Issue 4 Pages 544-548
Published: August 28, 2000
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSA 35-year old woman underwent splenectomy due to a large splenic cyst which was identified by ultrasonographic screening. The splenic cyst was 7.5 cm long in diameter and its contents showed high levels of CA19-9, LDH, and total cholesterol. The cystic wall consisted of fiberous connective tissue with hyaline degeneration and calcification, and foamy macrophages as a consequence of cholesterol phagocytosis had accumulated with inflammatory cell infiltration in the wall, mimicking chronic inflammation in the atherosclerotic lesion. The lesion was diagnosed as a calcified true cyst of the spleen based on the findings of epithelial cells in rare areas of the cystic wall. It is suggested that accumulation of foamy macrophages as a consequence of cholesterol phagocytosis with inflammatory cell infiltration may cause epithelial cell degeneration and necrosis, and develop calcification in a true splenic cyst showing a high level of cholesterol.View full abstractDownload PDF (4071K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...2000 Volume 60 Issue 4 Pages 549-551
Published: August 28, 2000
Released on J-STAGE: September 09, 2010
JOURNAL FREE ACCESSDownload PDF (373K)
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