Silica gel thin-layer chromatography was applied to detect and isolate aliphatic alcohols, free from hydrocarbons, from small amounts of the unsaponifiable matter of lipids. The conditions of the chromatography were established by using known alcohols, and were applied to natural alcohols and waxes obtaining the following results. 1) For the separation of the even-numbered (C10-C18) alcohols from added hydrocarbons, petroleum benzine : ether (4 : 1vol/vol); n-hexane : ether (7 : 3vol/vol); n-hexane : ether : acetic acid (7 : 3 : 0.1vol/vol); and xylene : ether (4 : 1vol/vol) were the best developing solvent systems. The alcohol mixture was secured in these systems in a single spot, free from contamination by hydrocarbons. 2) This technique allowed enough to detect 35μg of alcohols. 3) Sperm blubber alcohol in which even-numbered, odd-numbered, saturated and unsaturated alcohols were known as the component also formed a single spot of the chromatogram. 4) This method was applied successfully for the unsaponifiable matter of whale wax, sperm head oil, wool wax, and beeswax.
Solubility measurement in organic solvents was applied on stigmasterol, non-stigmasterol and these mixtures. These results are given in tables and figures. Separation of stigmasterol from the sterols mixtures was found difficult by inoculation method. From these data, stigmasterol and non-stigmasterol showed a behavior like a solid solution in crystalline form. However, this behavior could not be confirmed by X-ray powder diffraction method.
Cellosolve acetate, methyl acetate, ethyl acetate, n-butyl acetate and iso-amyl acetate were investigated for alkali hydrolysis at 20 and 30°C, and for acid hydrolysis at 70°C. More positivity of the ester combination resulted higher reaction velocity in case of saponification reaction. In the case of acid catalized hydrolysis the velocity of reaction was very low, and cellosolve acetate did not react so higher than the methyl acetate. Cellosolve acetate had higher velocity of hydrolysis than other alkyl acetate in hydrophility.
It has been known that aqueous solutions of polymeric surfactants behave peculiarly as compared with ordinary monomeric surfactants. However, the phenomena is still imcompletely explored. Authors contemplated study for clarifying the relationship between molecular weights of polymeric surfactants derived from nonylphenol-formaldehyde condensares and their properties in aqueous solutions. In this paper, the reaction of formaldehyde with nonylphenol in ethanol solution was investigated. As a result, the order of the reaction was considered to be secondary to the cocnentration of formaldehyde, and then the activation energy of 22.4kcal was calculated. Nobolac type resins of various molecular weights were easily prepared by homogenious reactions using n-butanol as solvent. Resins were then polyoxyethylated. Polymeric surfactants thus obtained differed from monomeric ones in many respects and excellent emulsification of xylol was attained by using polymeric surfactants, especially for nonylphenol-formaldehyde condensate having mean molecular weight around 1, 000.