Silica gel thin-layer chromatography was applied to detect and isolate aliphatic alcohols, free from hydrocarbons, from small amounts of the unsaponifiable matter of lipids.
The conditions of the chromatography were established by using known alcohols, and were applied to natural alcohols and waxes obtaining the following results.
1) For the separation of the even-numbered (C
10-C
18) alcohols from added hydrocarbons, petroleum benzine : ether (4 : 1vol/vol);
n-hexane : ether (7 : 3vol/vol);
n-hexane : ether : acetic acid (7 : 3 : 0.1vol/vol); and xylene : ether (4 : 1vol/vol) were the best developing solvent systems. The alcohol mixture was secured in these systems in a single spot, free from contamination by hydrocarbons.
2) This technique allowed enough to detect 35μg of alcohols.
3) Sperm blubber alcohol in which even-numbered, odd-numbered, saturated and unsaturated alcohols were known as the component also formed a single spot of the chromatogram.
4) This method was applied successfully for the unsaponifiable matter of whale wax, sperm head oil, wool wax, and beeswax.
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