Polar fractions of fatty acid methyl esters (PFA) prepared from soybean oil heated 30 h by frying whale meat (Wh) and potato (Po) were subjected to fractionation by TLC and silicagel column chromatography (CC), monitoring each fraction for its UV-absorbance (at 233 nm). Highly UV-active fractions were analyzed by GLC and GC-MS to compare with the chromatographic patterns obtained from Wh and Po and to comprehend the nature of UV-active components. The fractions with Rƒ in a range of 0.230.96 by TLC (developed with petroleum ether-diethyl ether-ethanol 50 : 50 : 2) contributed nearly 90% of the total UV-absorption of PFA and amounted 5669%(on the weight basis) of PFA. These fractions showed strong intensities of absorption at 790795 and 765770 cm-1 in the IR region. The chromatograms obtained from both samples were quite similar, indicating similar nature of components being involved in both samples. The most remarkable UV-active subfractions obtained by CC (Rƒ 0.810.96) as well as five subsequent fractions (Rƒ up to 0.73) were found to be composed of short chain esters, chiefly C11C15, having UV-active conjugated systems such as diene, enal (or enone), and formylenol. Compounds having a carbonyl or a hydroxyl group in their mid-chains were also detected in GLC fractions eluted at high temperatures. On the other hand, a subfraction with its Rƒ 0.590.67 was found to be composed of chiefly C18 compounds either in an normal chain or cyclohexyl form having the conjugated systems as mentioned above and also a keto or hydroxyl group. Other compounds found were 8-phenyloctanal, C6 and C8-esters carring in their molecules the 5-oxo-1-cyclopentenyl residue, and dioctyl phthalate.
Crystalline Glu, Lys and Cys had the antioxidative activities and inhibited the oxidative activity of ferrous chloride by the Active Oxygen Method (98°C). The inhibitory activities of the mixtures of L-amino acids containing Glu, Lys or Cys on the oxidative activity of ferrous chloride were proportional to those of L-amino acids described above. The inhibitory activities of peptides on the oxidative activity of metal salt were not proportional to those of the mixtures of the constituent L-amino acids. Polypeptides and proteins had small inhibitory activities on the oxidative activity of iron (II) chloride in comparison with Glu or Lys. Peptides and the mixtures of the constituent L-amino acids inhibited the oxidative activity of copper (II) sulfate. Iron and copper seem to adhere loosely on the surface of crystalline peptides under the conditions of Active Oxygen Method on the basis of IR spectra of the obtained metal-peptide complexes. However, the loss of the oxidative activities of metal salts was nots proportional to the amounts of metal in the metal-peptides or metal-L-amino acids complexes.
Poly (methylacrylate) (PMA) prepared by radical polymerization of methylacrylate was allowed to react with 2-aminoethanol (AE) to obtain water soluble polymer “PMA-AE”. This polymer reacts with copper (II) ion in dilute aqueous solution to form complex which has a good foaming ability. The visible spectra of this complex slightly change with pH. The best removal effect of copper was obtained by foam treatment at pH 6 where the complex exhibits its absorption maximum, More than 85% of copper was recovered selectively at the same pH when (PMA-AE) was used for the foam treatment of a model leaching solution of manganese nodules.
The creep behavior of vulcanized natural rubber under the stress has been studied in aqueous solutions of surfactants, sodium dodecyl sulfate (NaC12S), octylphenyl polyoxyethylene ether (C8PhE9) and nonyl phenyl polyoxyethylene ether (C9PhE15). The creep elongation of the rubber in the presence of a surfactant was much larger than that in air or water, and the extent of the elongation depended on the type and the concentration of surfactants. In particular, the effect of C9PhE15 on the creep elongation was remarkable. These results may be interpreted as showing that a surfactant in solution promotes the wetting of the rubber surface and, at the same time, the network structure in the crosslinked rubber is loosened by the penetration of surfactant molecules into the structure. The results for wetting of the rubber by various types of organic solvents under the stress have shown that the critical surface tension (γc) of the rubber slightly increases with the strain, suggesting an increase of the surface free energy of the rubber increasing with the strain.
A simple method for determination of oil content and fatty acid composition in small seed sample (>2mg) is presented using methyl heptadecanoate as internal standard. Using this method, the possibility f loss of oil is eliminated, and simultaneous extraction and methyl esterification of oil in a test tube can be efficiently carried out. A comporison of the present method with conventional one revealed a close agreement with regard to oil content and fatty acid composition of two majou rapeseed crops, viz., Brassica napus and Brassica campestris.
A new preparative method of bicyclic ethers from diolefinic alcohols and acidic materials was described. 1-Octen-5-one was prepared from butyric acid and vinylmagnesium chloride. Reaction of 1-octen-5-one with vinylmagnesium chloride afforded 3-hydroxyl-3-propyl-1, 6-heptadiene. 1-Propyl-8-oxa bicyclo [3, 2, 1] octane was obtained by treating 3-hydroxyl-3-propyl-1, 6-heptadiene with a mixture of formic acid and 7O% sulfuric acid.