While a few reports on the component fatty acids of the baleen whale oils by gas-liquid chromatography (GLC) have been published, no detailed paper including the minor constituents on the antarctic whale oil has been found yet. The purpose is minute examination of the component fatty acid of the antarctic sei whale blubber oil by GLC using a hydrogen flame ionization detector. Two samples of the oil were converted to methyl esters by alkali-catalyzed methanolysis, and the resulting methyl esters were separated by preliminary urea complex fractionation and by subsequent silicic acid column chromatography or thin layer chromatography on silica gel impregnated with silver nitrate. Each fraction was then analyzed by GLC on a polyester column before and after hydrogenation, as previously reported. As a result, qualitative evidence was confirmed for the presence of more than 45 fatty acids ranging from 12 to 24 carbon atoms in chain length. Included were the esters of the following : Normal saturated acids (12, 13, 14, 15, 16, 17, 18, 19, 20, 21 and 22 C-atoms), branched-chain fatty acids (14, 15, 16, 17 and 18 C-atoms), monoenoic acids (12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23 and 24 C-atoms), dienoic acids (14, 16, 18 and 20 C-atoms), trienoic acids (16, 18, 20 and 22 C-atoms), polyenoic acids (16 : 4, 17 : 4, 18 : 4, 20 : 4, 22 : 4, 19 : 5, 20 : 5, 21 : 5, 22 : 5, 22 : 6 and 24 : 6), and a large number of unknown minute components. As above mentioned, it is noteworthy that polyunsaturated odd-numbered and branched-chain fatty acids are present in the constituents of sei whale oil.
In the previous paper, the yield of glycerine was found to be decreased by increasing the concentration of sodium hydroxide in hydrolysis of epichlorohydrin. In this paper, the velocity of element reactions in the hydrolysis of epichlorohydrin was investigated and was found that the ring opening of glycidol has major effect on glycerine synthesis. The hydrolysis of glycidol is the first order reaction with concentrated sodium carbonate, but is not so with a concentrated sodium hydroxide, and which facts are considered due to that there exists such a side reaction as polymerisation of glycidol in case of the latter. Viewed from this connection, it can be concluded that the decrease in the yield of glycerine reported in the previous paper depends on the side reaction occuring in the course of glycidol hydrolysis.
In atherosclerosis, the most popular type of arterosclerosis, the deposit of cholesterol or cholesterol esters in the artery were present, but the cause of the deposit is still obscure. The lipids in the plasma are present as lipo-proteins. Generally, this combination of lipids and proteins is not strong. However, lipids, in which oxidized unsaturated fatty acids are present, and proteins react to form the lipid-protein complexes, with the strong combinning forces between the both undecomposed by the solvent extraction. If oxidized lipids form the complex with the proteins of the artery, it may be presumed that cholesterol and fatty acid cholesterol esters may be deposited around the complex. It was previously shown by the authors that trans-trans conjugated diene hydroperoxides are present in the lipids of atherosclerotic breast aorta. As the cause of atherosclerosis outbreak, the author presents a hypothesis that, “the oxidized fats form the complex with the proteins in aorta vessel, and then the lipids, such as cholesterol or cholesterol esters, are deposited around the complex in the artery” (written as (A) in the undergoing). It was previously shown by the authors' study that oxidized lipids are concentrated in the phospholipids of atherosclerosic aorta lipids, that they contain trans-traps conjugated diene hydroperoxides, that the lipid-protein complexes are present in atherosclerosic abdominal aorta, and that oxidized lipids containing traps isomers are present in the lipids combining with the aorta proteins. These facts are evidence of (A). Athero sclerosis broke out in the aorta of the rabbit by feeding it with the yolk of egg or with the brain of cattle. This can be explained by (A). It was reported that the deposit of lipids in artery has relation to lipo proteins in plasma. This can be understood by (A). Though Japanese take small amounts of fat, many are suffering from atherosclerosis. This cause can be explained by (A). Atherosclerosis also breakes out by taking a large amount of fat or cholesterol, by removing thyroid gland, and by feeding the food absence vitamin B6. These causes can be also understood by (A). By dosing vitamin G, pantothenic acid, or a large amount of proteins, atherosclerosis outbreak is restrained. This can be explained by (A). Thus the hypothesis (A) may be valuable to afford general understanding of atherosclerosis outbreak.
Several organo-phosphorus derivatives were synthesized from fatty acids and alcohols and their rust-preventive properties were examined by three corrosion tests, such as salt immersion test, out-door exposure test and humidity cabinet test. As a result, the derivatives having free phosphonic or phosphonous acid group were hardly soluble in base oil, but when used together with sorbitanmonooleate, they dissolved in base oil and showed good rust-preventive properties. Especially, 9 (10) phosphonostearic acid, 9 (10) phosphonostearyl alcohol, 9 (10) - (hydroxyphosphinyl) stearic acid and 9 (10) - (hydroxyphosphinyl) stearyl alcohol were surpassing.
Previous analytical studies of menthol steroisomers by means of thermoconductive gas chromatography demonstrated the difficulties in separation. The present paper deals with analysis of menthol streoisomers by capillary gas chromatography with flame ionisation detector, utilizing P.E.G. 4000, Reoplex 400, Lac-2 R-446, Hyprose SP 80 and D.O.S as the capillary substrate. The experimental conditions are as follows : a coated capillary coloumn (dia. 0.2mm, length 30-50m) prepared by the authors, coloumn temp. 120-130°C and split temp. 250°C. The coloumns of P.E.G. 4000, Reoplex 400 and Lac-2R-446 afford significantly better resolution than Hyprose SP 80 and D.O.S. as shown in Table-2.
Colouring of polyoxyethylene alkylamine during the production process was studied with RNH2, RN (CH3) 2, RN (C2H4OH) 2 and ROH each of which was reacted with ethylene oxide containing various amount of acetaldehyde. In the case using RNH2, the colouring of the adduct was depressed till 2 mol addition. Tert-amino group accelerated colouring of the adduct. It seems that the colouring of polyoxyethylene alkylamine is not due to formation of azomethine group and main cause of the colouring is another reaction of acetaldehyde such as polymerization and it's decomposition etc.