Nonylphenol-formaldehyde condensates whose molecular weights ranged from 527 to 793 were prepared by the condensation of p-nonylphenol with formaldehyde and were allowed to react with ethylene oxide to yield oligomeric surface active agents. Then f ormyl, nitro or amino group was introduced into these products according to the procedure reported in the previous paper. The surface tensions of aqueous solutions of each derivatives were determined and it was found that the surface tensions lowered to about 3235 dyn/cm. Their antimicrobial activities against gram-positive and gramnegative bacilli and fungi were evaluated in terms of minimum inhibitory concentration by a dilution method. Formyl derivatives had the inhibitory activity against gram-negative bacilli and fungi, on the other hand dinitro and diamino derivatives against gram-positive bacilli and fungi. But these oligomeric surface active agents were less active than the monomeric ones, which had the corresponding oxyethylene chain length and the same functional group. No difference in antimicrobial activity was observed in these oligomeric surface active agents which had different molecular weight.
The solubilization of ethanol, acetone and methyl acetate in single and mixed states by dodecylammonium propionate (DAP) in toluene was studied by the aid of GLC. The effect of addition of imidazole on the solubilization of ethanol and acetone by DAP was also studied. The following results were obtained : (1) The amount of ethanol solubilized by DAP increases by the presence of acetone or methyl acetate. (2) The amount of acetone and methyl acetate solubilized by DAP increases by the presence of ethanol. (3) The amount of ethanol and acetone solubilized by DAP increases by addition of imidazole.