An attempt to quantitate the tocopherol content in the autoxidized methyl linoleate was made by the iron (III) chloride-2, 2'-bipyridine method. But the quantitation was very difficult owing to the interference of hydroperoxides. This interference was also observed in t-butyl and cumene hydroperoxides, but not in di-t-butyl and dicumene peroxides. Such hydroperoxides as autoxidized methyl linoleate, t-butyl and cumene hydroperoxides catalyzed the Emmerie-Engel color reaction by itself as if it contained tocopherols. This result is contradictory to the earlier studies that the peroxides in fats inhibited the color development in the Emmerie-Engel procedure. Tocopherols in autoxidized methyl linoleate were quantitatively determined when hydroperoxides were removed by the potassium iodide treatment.
The effects upon surface tension for the aqueous solution of sodium dodecyl sulfate (SDS) by concentration of calcium and triphosphate ions were investigated. The results were interpreted by change of surface adsorption for dodecyl sulfate salt under concentration of sodium tripolyphosphate (STP). Interfacial tension between the solution mentioned above and two sorts of oil, i. e. oleic acid and liquid paraffin, and the contact angle of the two liquid interface on polyester films were measured. Residual works of detergency for these systems were calculated from these values and the effects of STP concentration upon the STP-hard water systems were discussed. It was concluded that the detergent builder effects of STP were dependent on types of oil. For oleic acid soil, soap formation in situ as an alkaline salt emulsified the oil itself. On the other hand, for paraffin droplet soil, they assisted to increase the contact angle on the film and to remove easily from the film by rolling-up mechanism.
The high speed liquid chromatography (HSLC) was adapted for the stability test of hydrocortisone acetate (HA) in emulsion. The HSLC conditions were as follows : Model : JASCO FLC-350, Detector : UVIDEC-1, wavelength : 242 nm, Column : JASCOPACK-SV-02-500, Mobile phase : MeOH : H2O (6 : 4), Flow rate : 1 ml/min, Pressure : 70 kg/cm2 and Chart speed : 1 cm/min (at room temperature). Thymolphthalein (TF) was used as a internal standard material. The extracted HA was directly injected to the HSLC column, in which the stationary phase was supported by octadecylsilane. Six kinds of emulsions containing 1 % HA, which are absorption and hydrophilic ointment of improved, pharmacopeia and commercial grades, were exposed to air at 10°C or 40°C, and then measured their stability to the oxidation by using HSLC. The result showed that the amount of HA in W/O emulsion decreased, but in O/W emulsion did not change. Aging effect on the oxidation of six kinds of emulsions was investigated by measuring peroxide value (POV). It was found that POV of W/O emulsions increased and that of O/W emulsions were constant.
Synthesis of citronellol from isoprene in three steps was reported. The reaction steps were consisted of three parts, i.e. a) Dimerization of isoprene was carried out in the presence of tetraethoxy zirconium, diethylaluminium chloride and triphenylphosphine as a catalyst system to afford 2, 6-dimethyl-1, 3, 6-octatiene [II]. b) The triene [II] was converted into 2, 6-dimethyl-2, 6-octadiene [III] by selective 1, 4-hydrogenation of the conjugated diene moiety of [II] catalyzed by (methyl benzoate) chromiumtricarbonyl under hydrogene atmosphere. c) Citronellol [IV] was obtained from [III] by use of hydrido bis (cyclopentadienyl) zirconium chloride (hydrozirconation reaction) and successive oxidation. Especially, in this report, reaction conditions such as molar ratio of the zirconium hydride to [III], solvent and reaction temperature were discussed. Total yield of citronellol base on [II] was ca. 50%.
Soybean oil fatty acid was esterified by the use of boron trifluoride-methanol reagent varying the relative amount of the reagent against the amount of fatty acid according to the specification of AOCS Ce 2-66. The yield of esters were analysed by GC. The values obtained under the different reaction conditions were treated statistically. It was found that there are the differences between the analytical values of unsaturated fatty acid obtained by the use of different amounts of the reagent against the same amounts of fatty acid. It was also observed that significant differences between the analytical values of unsaturated fatty acid was found when fatty acid sample were treated with different lots of the reagent under the same conditions.
The effect of the distribution of polyoxyethylene chain, and the length of alkyl group on the stability of W/O emulsions made by polyoxyethylene type nonionic surfactants, liquid paraffin, and water were investigated. The stability was examined at temperature of 0 to 100°C The stable W/O emulsions were given by the surfactants having ethyleneoxide distribution without long chain adducts, and having long chain alkyl group. But it is necessary that surfactants are liquid state in emulsion system at store temperature.