The illuminant color needs to be estimated for obtaining the color of an object stably against to illuminant changes. In order to investigate the efficiency of the optimal color model, which the authors have proposed, the model was tested whether it was sufficiently applicable to the real colors observed in natural environment. Measuring and analyzing the spectral radiations of daylight in the sun and the shade and spectral reflectance of many natural objects, we applied the optimal color models to the chromaticity and luminance distribution of those object under each illuminant. The results showed that the model estimated the illuminant colors quite well even when the object colors in the scene were largely biased in the hue.